We narrowed to 9,202 results for: sacs

1. iRFP713-FRB-Mss4-Kina

   Plasmid

   #202744

   Purpose

   expresses recruitable fluorescent protein-tagged enzyme

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   iRFP713:MTOR(2021-2113):GGSA2:S. cerevisiae Mss4 (377-756) (MSS4 Budding Yeast)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. 907L

   Plasmid

   #160237

   Purpose

   FlightN-ttgR-VP16-Sv40-attB

   Depositor

   Omar Akbari

   Article

   Gamez et al Insect Mol Biol. 2021 Oct;30(5):461-471.

   Inserts

   TtgR repressor
   Flightin promoter
   

   Use

   Tags

   VP16

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYIAL31

   Plasmid

   #60807

   Purpose

   used to make mutations in yeast POL1

   Depositor

   Thomas Kunkel, Youri Pavlov

   Article

   Pavlov et al Genetics. 2001 Sep;159(1):47-64.

   Insert

   Pol1 (POL1 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N-terminal part only

   Promoter

   Available since

   Feb. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJCSUP35(1-122)

   Plasmid

   #1091

   Depositor

   Susan Lindquist

   Article

   Glover et al Cell 1997 May 30;89(5):811-9.

   Insert

   SUP35 (SUP35 Budding Yeast)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   amino acids 1 - 122 of SUP35

   Promoter

   Available since

   Jan. 21, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFB-Flag-MyoD

   Plasmid

   #25994

   Depositor

   Jeffrey Dilworth

   Article

   Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

   Insert

   MyoD (Myod1 Mouse)
   

   Use

   Baculovirus transfer vector

   Tags

   Flag

   Expression

   Mutation

   Promoter

   Available since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pFA6a-link-GFPγ-SpHis5

   Plasmid

   #60781

   Purpose

   Yeast C-terminal tagging vector, which creates gene fusions with GFPγ using SpHis5 selection

   Depositor

   Linda Huang

   Article

   Slubowski et al Yeast. 2015 Jan 22. doi: 10.1002/yea.306

   Insert

   GFPγ
   

   Use

   Yeast integrating

   Tags

   Expression

   Mutation

   T65C S72A V163A I167T L231H

   Promoter

   Available since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. ptagBFP-LacI-NLS-VP16

   Plasmid

   #103837

   Purpose

   Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   tagBFP-LacI-NLS-VP16
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV2

   Available since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pUDP003

   Plasmid

   #101164

   Purpose

   E. coli/S. cerevisiae amdS shuttle vector allowing cloning of ribozyme flanked g-RNA for Cas9 editing (HH-gRNA-HDV)

   Depositor

   Jean-Marc Daran

   Article

   de Vries et al Microb Cell Fact. 2017 Dec 5;16(1):222.

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   ScTDH3

   Available since

   Dec. 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-pEF1a-rtTAc

   Plasmid

   #172121

   Purpose

   Constitutive expression of InteinC-rtTAC

   Depositor

   Chun Xu

   Article

   Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

   Insert

   InteinC-rtTAc
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. HcKan_T

    Plasmid

    #65316

    Purpose

    receiving vector of terminators for the construction of standard terminator parts in YeastFab work

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. HcKan_O

    Plasmid

    #65315

    Purpose

    receiving vector of ORFs for the construction of standard ORF parts in YeastFab work

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. HcKan_P

    Plasmid

    #65314

    Purpose

    receiving vector of promoters for the construction of standard promoter parts in YeastFab work

    Depositor

    Junbiao Dai

    Article

    Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

    Insert

    RFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. HsCERK

    Plasmid

    #118600

    Purpose

    Expresses ceramide kinase, which catalyzes the phosphorylation of ceramide to form ceramide 1-phosphate

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Homo sapiens ceramide kinase
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Feb. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLIK 3XFLAG-MKK4-EE-NLS hygro

    Plasmid

    #87779

    Purpose

    Inducible expression of constitutively active mutant MKK4 with a C-terminal NLS sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK4
    

    Use

    Lentiviral

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 31, 2017

    Availability

    Academic Institutions and Nonprofits only

15. Sc NybbR eRF3∆165N (WT)

    Plasmid

    #175419

    Purpose

    Encodes His-MBP-TEV-ybbR-WT eRF3∆165N

    Depositor

    Joseph Puglisi

    Article

    Lawson et al Science. 2021 Aug 20;373(6557):876-882.

    Insert

    eRF3 (SUP35 Budding Yeast)
    

    Use

    Tags

    His-MBP-TEV and ybbR

    Expression

    Bacterial

    Mutation

    ∆165N

    Promoter

    T7

    Available since

    Oct. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS_FLPER(T)

    Plasmid

    #31131

    Depositor

    Gerhart Ryffel

    Article

    Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

    Insert

    FLP-ER ligand binding domain (ESR1 Budding Yeast, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    full length FLP linked to ligand binding domain o…

    Promoter

    Available since

    Oct. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYES2.1-cerMET5

    Plasmid

    #107437

    Purpose

    Overexpression of MET fusion protein in S. cerevisiae

    Depositor

    Clare O'Connor

    Article

    Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

    Insert

    MET5 (MET5 Budding Yeast)
    

    Use

    Tags

    V5-His

    Expression

    Yeast

    Mutation

    V81I, K341E, P597L, Y605C, E741G, D932G, N1217S, …

    Promoter

    GAL1

    Available since

    Aug. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. EC0_2_VP64

    Plasmid

    #163714

    Purpose

    Plasmid encoding VP64 - SV40 NLS as a Type 4a part to be used in the Dueber YTK system (MoClo kit)

    Depositor

    Yvonne Nygård

    Article

    Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

    Insert

    VP64 + SV40 NLS
    

    Use

    Synthetic Biology; Moclo vector level 0 (type 4a)

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRS303 Bt δCOP

    Plasmid

    #73871

    Purpose

    Bos taurus Arcn1

    Depositor

    Blanche Schwappach

    Article

    Arakel et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    Ret2 Promoter- COPD
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Ret2 Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Plas-CRISPR_reporter

    Plasmid

    #157658

    Purpose

    CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

    Depositor

    Frank Albert

    Article

    Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

    Insert

    dCAS9-VP64, mCherry, KanMX
    

    Use

    Insert storage (replicative in e. coli)

    Tags

    Expression

    Mutation

    N28D in mCherry- please see depositor comments be…

    Promoter

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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21. p alpha-actinin:GAL4/UAS:TdTomato

    Plasmid

    #161712

    Purpose

    Express TdTomato through amplification of GAL4/UAS system

    Depositor

    Hollis Cline

    Article

    Hiramoto et al Proc Natl Acad Sci U S A. 2014 Nov 25;11

    Insert

    TdTomato, GAL4
    

    Use

    Xenopus laevis expression

    Tags

    TdTomato

    Expression

    Mammalian

    Mutation

    Promoter

    actin-GAL4, UAS

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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22. pILGFP3A2 (2)

    Plasmid

    #185852

    Purpose

    Testing the TEF1 promoter appended with 3 tetracycline riboswitch using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1>3*TcRb>yEGFP>TPGK1-TURA3
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDYSCKO

    Plasmid

    #120263

    Purpose

    Empty backbone for cloning any gRNA to be used with the canavanine co-selection system

    Depositor

    Christian Landry

    Article

    Despres et al G3 (Bethesda). 2018 Oct 3;8(10):3163-317

    Insert

    DYSCKO cassette
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    SNR52

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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24. pHB4

    Plasmid

    #53462

    Purpose

    This plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.

    Depositor

    Elizabeth Conibear

    Article

    Burston et al J Cell Biol. 2009 Jun 15. 185(6):1097-11

    Insert

    yeGFP-Snc1-Suc2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ADH

    Available since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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25. pGL3_4xUAS_CP-candidate_luc+

    Plasmid

    #125154

    Purpose

    STAP-seq luciferase validation vector, which harbors an 4x UAS array upstream of the CP position (candidates are inserted at CP position) for the recruitement of GAL4-DBD tagged proteins

    Depositor

    Alexander Stark

    Article

    Haberle et al Nature. 2019 Jun;570(7759):122-126. doi:

    Insert

    4 x upstream activating sequence (UAS)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AB.pCCL.sin.cPPT.U6.miR-182-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46596

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-182 Decoy
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

27. AAV_A2S-8

    Plasmid

    #188654

    Purpose

    AAV vector expressing SaAID-2S and gRNA

    Depositor

    Keiji Nishida

    Article

    Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

    Inserts

    AID2S--nSaCas9-UGI
    Sa-sgRNA
    

    Use

    AAV and CRISPR

    Tags

    UGI

    Expression

    Mammalian

    Mutation

    D10A for SaCas9

    Promoter

    Scp1 and U6

    Available since

    Oct. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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28. pRS426-TUB1-internal6xHis

    Plasmid

    #60390

    Purpose

    Expression of yeast alpha tubulin (TUB1) - internal 6xHis using a GAL promoter

    Depositor

    Ron Vale

    Article

    Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

    Insert

    TUB1 (TUB1 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    internal 6xHis (see supplement figure 4)

    Promoter

    GAL

    Available since

    Jan. 26, 2015

    Availability

    Academic Institutions and Nonprofits only

29. AB.pCCL.sin.cPPT.GFP.miR-617.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85921

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-617 Sensor (MIR617 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLX302-PTP-SL-V5 puro

    Plasmid

    #87774

    Purpose

    Expresses V5 tagged human PTP-SL

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    PTP-SL (PTPRR Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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