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We narrowed to 16,979 results for: She

Showing: 9651 - 9700 of 16979 results

  2. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

    Plasmid
    #117140
    Purpose
    Express gRNA vs Dmap1
    Depositor
    Kosuke Yusa
    Insert
    gDmap1 v1 (Dmap1 Synthetic)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    Deleted BbsI site within WPRE, modified BFP
    Promoter
    human U6 promoter, mouse PGK promoter
    Available since
    April 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    117140-plasmid-map-sequence-id-233768

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  3. pTH825-CEN-minRLuc/slowstaCFLuc

    Plasmid
    #115370
    Purpose
    Expresses translation elongation-controlled Renilla luciferase and translation initiation-controlled firefly luciferase
    Depositor
    Tobias von der Haar
    Inserts
    Gcn4 5'-UTR + Firefly Luciferase
    Renilla luciferase (codon minimised)
    Use
    Tags
    Expression
    Yeast
    Mutation
    All codons have been changed for the least favour…
    Promoter
    ADH1 and TDH3
    Available since
    March 21, 2019
    Availability
    Academic Institutions and Nonprofits only
    115370-plasmid-map-sequence-id-238823

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  4. pCSD_FKBP-C-SpCas9MT3-574-1368-NLS-3XHA-NLS-TALETS3

    Plasmid
    #107301
    Purpose
    Expresses C terminal (574-1368 aa) R1335K mutant SpCas9 fused to FKBP and TALE-VEGFA-TS3 in mammalian cells
    Depositor
    Scot Wolfe
    Insert
    Truncated SpCas9 (aa 574-1368) fused to FKBP and TALE
    Use
    CRISPR
    Tags
    FKBP, SV40NLS, 3xHA, c-Myc NLS, and TALE_VEGFA-TS…
    Expression
    Mammalian
    Mutation
    Truncated SpCas9 (aa 574-1368) with R1335K mutati…
    Promoter
    CMV IE94
    Available since
    Nov. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107301-plasmid-map-sequence-id-204785

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  6. pBABE.puro/TO/Flag/HuR.S221D

    Plasmid
    #110347
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG tag
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    S221D, P237S
    Promoter
    gag
    Available since
    Aug. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110347-plasmid-map-sequence-id-218849

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  8. pBABE.puro/TO/Flag/HuR.S221D

    Plasmid
    #110355
    Purpose
    Doxycycline inducible mammalian retroviral expression of HuR (S221D) with FLAG and mKO2 tags
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAV like RNA binding protein 1 (ELAVL1 Human)
    Use
    Retroviral
    Tags
    FLAG and mKO2
    Expression
    Mammalian
    Mutation
    S221D, P237S
    Promoter
    gag
    Available since
    June 22, 2018
    Availability
    Academic Institutions and Nonprofits only
    110355-plasmid-map-sequence-id-213699

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  15. pcDNA3.1 Flag YFP hNKCC1 A720C C723S C724V (NT534)

    Plasmid
    #50899
    Purpose
    Expresses human NKCC1 A720C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    A720C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50899-plasmid-map-sequence-id-76372

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  16. pcDNA3.1 Flag YFP hNKCC1 L718C C723S C724V (NT532)

    Plasmid
    #50897
    Purpose
    Expresses human NKCC1 L718C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L718C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50897-plasmid-map-sequence-id-76370

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  17. pcDNA3.1 Flag YFP hNKCC1 L722C C723S C724V (NT536)

    Plasmid
    #50901
    Purpose
    Expresses human NKCC1 L722C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L722C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50901-plasmid-map-sequence-id-76374

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  18. pcDNA3.1 Flag YFP hNKCC1 S716C C723S C724V (NT530)

    Plasmid
    #50895
    Purpose
    Expresses human NKCC1 S716C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    S716C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50895-plasmid-map-sequence-id-76368

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  19. pcDNA3.1 Flag YFP hNKCC1 I721C C723S C724V (NT535)

    Plasmid
    #50900
    Purpose
    Expresses human NKCC1 I721C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I721C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50900-plasmid-map-sequence-id-76373

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  20. pcDNA3.1 Flag YFP hNKCC1 C723S C724V W732C (NT546)

    Plasmid
    #50908
    Purpose
    Expresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V W732C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50908-plasmid-map-sequence-id-76381

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  21. pcDNA3.1 Flag YFP hNKCC1 C723S C724V I725C (NT538)

    Plasmid
    #50903
    Purpose
    Expresses human NKCC1 C723S C724V I725C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C722S C723V I724C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50903-plasmid-map-sequence-id-76376

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  22. pcDNA3.1 Flag YFP hNKCC1 C723S C724V V726C (NT539)

    Plasmid
    #50904
    Purpose
    Expresses human NKCC1 C723S C724V V726C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S C724V V726C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50904-plasmid-map-sequence-id-76377

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  23. pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)

    Plasmid
    #50898
    Purpose
    Expresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    G719C C723S C724V in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50898-plasmid-map-sequence-id-76371

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  24. pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)

    Plasmid
    #49062
    Purpose
    Expresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and mVenus
    Expression
    Mammalian
    Mutation
    N-terminal deletion of amino acids 13-222 in hNKC…
    Promoter
    CMV
    Available since
    Nov. 1, 2013
    Availability
    Academic Institutions and Nonprofits only
    49062-plasmid-map-sequence-id-72184

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  27. BDH2

    Plasmid
    #39151
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    BDH2 (BDH2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  28. MAGI1

    Plasmid
    #38882
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGI1 (MAGI1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C890S; codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  29. MAGI1

    Plasmid
    #38883
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGI1 (MAGI1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C1189S; codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  30. MAGI1

    Plasmid
    #38901
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAGI1 (MAGI1 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    C677S, C709S; codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  31. DMTF1

    Plasmid
    #36895
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DMTF1 (DMTF1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Myb-like 1 domain, residues 220-274
    Promoter
    Available since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. HaloTag-beta2-adrenergic receptor

    Plasmid
    #66994
    Purpose
    Beta-2-adrenergic receptor tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    HaloTag-beta2-adrenergic receptor (ADRB2 Human)
    Use
    Tags
    Human Growth hormone Signal Sequence-HaloTag
    Expression
    Mammalian
    Mutation
    The signal sequence from human growth hormone was…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    66994-plasmid-map-sequence-id-115414

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  37. OA984-HA

    Plasmid
    #120362
    Purpose
    Expresses a his-tagged single chain antibody 1C19 that targets all four DENV serotypes and expresses red fluorescent protein reporter
    Depositor
    Omar Akbari
    Article
    Buchman et al PLoS Pathog. 2020 Jan 16;16(1):e1008103.
    Insert
    1C19 his-tagged single chain antibody
    Use
    Unspecified
    Tags
    Expression
    Mutation
    Promoter
    AAEL010782 carboxypeptidase A (CPA) gene Promoter
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    120362-plasmid-map-sequence-id-234087

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  39. pRVL-3

    Plasmid
    #104581
    Purpose
    Contains mouse IgG2c CH1/mutated CH2/CH3 domains, for cloning of antibody VH domains using SapI restriction enzyme. Abolished Fc-mediated immune effector functions
    Depositor
    Daniel Christ
    Article
    Vazquez-Lombardi et al Methods Mol Biol. 2018;1827:313-334. doi
    Insert
    Mouse IgG2c CH1/CH2/CH3 (mutated, no effector functions)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L234A/L235E/G237A and E318A/K320A/K322A
    Promoter
    CMV
    Available since
    Jan. 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    104581-plasmid-map-sequence-id-201392

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  43. mCherry-NbALFA

    Plasmid
    #159987
    Purpose
    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mCherry-NbALFA
    Use
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mCherry and NbALFA
    Promoter
    Available since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159987-plasmid-map-sequence-id-314745

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  45. pUC57-mitoTPNOX

    Plasmid
    #87854
    Purpose
    This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.
    Depositor
    Vamsi Mootha
    Article
    Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/
    Insert
    mitoTPNOX
    Use
    Tags
    C-terminal FLAG tag with a linker and mitochondri…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    87854-plasmid-map-sequence-id-175127

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  46. pCMV-T7-ABE8e-nSpCas9-VRQR-P2A-EGFP (KAC1309)

    Plasmid
    #185913
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9-VRQR(D10A/D1135V/G1218R/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Insert
    human codon optimized ABE8e-nSpCas9-VRQR-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9-VRQR=D10A/D1135V/G1218R/R1335Q/T1337R
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    185913-plasmid-map-sequence-id-367365

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  48. pTipi2.1_Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa

    Plasmid
    #221356
    Purpose
    Mammalian expression of Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa; to be used with Anti-Strep [IPI-C23.21] heavy chain (Plasmid 221355) to make the antibody.
    Depositor
    Institute for Protein Innovation (IPI)
    Insert
    Anti-Strep [IPI-C23.21] Mouse/Rabbit LC kappa
    Use
    Affinity Reagent/ Antibody
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 1, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    221356-plasmid-map-sequence-id-436814

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Showing: 9651 - 9700 of 16979 results