We narrowed to 44,096 results for: cha

1. NHA-Delta 12b

   Plasmid

   #36934

   Depositor

   Edward Chan

   Article

   Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

   Insert

   TNRC6A Delta 12b (TNRC6A Human)
   

   Use

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   aa 1045-1219 only

   Promoter

   Available Since

   May 30, 2012

   Availability

   Academic Institutions and Nonprofits only

2. NHA-Delta 12a

   Plasmid

   #36933

   Depositor

   Edward Chan

   Article

   Yao et al Nucleic Acids Res. 2011 Apr;39(7):2534-4

   Insert

   TNRC6A Delta 12a (TNRC6A Human)
   

   Use

   Tags

   NHA

   Expression

   Mammalian

   Mutation

   aa 896-1045 only

   Promoter

   Available Since

   May 30, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pGSTag BimL(AAA)

   Plasmid

   #25063

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   BimL (AAA) (BCL2L11 Human)
   

   Use

   Tags

   Gst

   Expression

   Bacterial

   Mutation

   Changed serine 44 to alanine Changed threonine 5…

   Promoter

   Available Since

   Aug. 13, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pCDNA3 T7 BimL (AAA)

   Plasmid

   #24244

   Depositor

   Roger Davis

   Article

   Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

   Insert

   Bim L(AAA) (BCL2L11 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   changed Serine 44 to Alanine changed Threonine 5…

   Promoter

   Available Since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

5. HOXB4-FLAG-pSP65

   Plasmid

   #20990

   Depositor

   Corey Largman

   Article

   Chang et al Genes Dev. 1995 Mar 15. 9(6):663-74.

   Insert

   HOXB4 (HOXB4 Human)
   

   Use

   In vitro trans/trans

   Tags

   Expression

   Mutation

   Missing first 14 amino acids

   Promoter

   Available Since

   Nov. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

6. GFP-D1a_W1-2(aa270-346)

   Plasmid

   #21524

   Depositor

   Edward Chan

   Article

   Lian et al RNA. 2009 May . 15(5):804-13.

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contain aa270-346

   Promoter

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

7. GFP-D1a_W2-3(aa318-399)

   Plasmid

   #21525

   Depositor

   Edward Chan

   Article

   Lian et al RNA. 2009 May . 15(5):804-13.

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contain aa318-399

   Promoter

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

8. GFP-D1a_W3-4(aa340-439)

   Plasmid

   #21526

   Depositor

   Edward Chan

   Article

   Lian et al RNA. 2009 May . 15(5):804-13.

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contain aa340-439

   Promoter

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

9. GFP-D1a_W4-5(aa409-495)

   Plasmid

   #21527

   Depositor

   Edward Chan

   Article

   Lian et al RNA. 2009 May . 15(5):804-13.

   Insert

   TNRC6A (TNRC6A Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   contain aa409-495

   Promoter

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

10. AF0837(128-241)

    Plasmid

    #19093

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Cell division protein pelota (DOM34 Archaeoglobus fulgidus)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

11. AF0837(242-344)

    Plasmid

    #19094

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Cell division protein pelota (DOM34 Archaeoglobus fulgidus)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

12. Dom34 E23K/F218A

    Plasmid

    #19106

    Depositor

    SeWon Suh

    Article

    Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

    Insert

    Protein DOM34 (DOM34 Budding Yeast)
    

    Use

    Tags

    C tag

    Expression

    Bacterial

    Mutation

    E23K/F218A

    Promoter

    Available Since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3 PARL-FLAG-CT S65A

    Plasmid

    #13618

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    PARL (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Serine 65 to Alanine

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3 PARL-FLAG-CT T69A

    Plasmid

    #13619

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Thr 69 to Ala

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3 PARL-FLAG-CT S65D

    Plasmid

    #13621

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 65 to Asp

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pcDNA3 PARL-FLAG-CT T69D

    Plasmid

    #13622

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Thr 69 to Asp

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA3 PARL-FLAG-CT S70D

    Plasmid

    #13623

    Depositor

    Luca Pellegrini

    Article

    Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

    Insert

    Presenilin-associated rhomboid-like (PARL, PARL Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed Ser 70 to Asp

    Promoter

    Available Since

    Jan. 12, 2007

    Availability

    Academic Institutions and Nonprofits only

18. SNX26-PX (56-172)

    Plasmid

    #119105

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX26-PX (56-172)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX26-PX (56-172)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. SNX28-PX (1-124)

    Plasmid

    #119107

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX28-PX (1-124)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX28-PX (1-124)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. SNX34-PX (1-130)

    Plasmid

    #119113

    Purpose

    Bacterial expression of human phox homology (PX) domain, SNX34-PX (1-130)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    SNX34-PX (1-130)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. IRAS-PX (13-124)

    Plasmid

    #119118

    Purpose

    Bacterial expression of human phox homology (PX) domain, IRAS-PX (13-124)

    Depositor

    Brett Collins

    Article

    Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

    Insert

    IRAS-PX (13-124)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pF(UG) hSyn NS34a-msGFP-'S-mito

    Plasmid

    #158768

    Purpose

    Lentiviral expression of self-cleaving membrane targeting peptide containing the OMP25 C-terminal targeting sequence, the P6P4 NS5A/5B cleavage site, msGFP and the NS3/4A protease.

    Depositor

    Edwin Chapman

    Article

    Vevea et al eLife. 2020 Jun 9;9. pii: 56469. doi: 10

    Insert

    NS3/4a protease and msGFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Flexible linkers between the protease and msGFP a…

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pFSW Syt1-C2B V304A,I367A IRES GFP

    Plasmid

    #133821

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    V304A, I367A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFSW Syt1-C2B D363,365N IRES GFP

    Plasmid

    #133822

    Purpose

    Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D363,365N

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pFSW Syt1-C2A K189-192A IRES GFP

    Plasmid

    #133823

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    K189-192A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pFSW Syt1-C2A D230,232N IRES GFP

    Plasmid

    #133824

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    D230,232N

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFSW Syt1-C2A M173,F234A IRES GFP

    Plasmid

    #133825

    Purpose

    Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

    Depositor

    Edwin Chapman

    Article

    Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

    Insert

    Syt1 (Syt1 Rat)
    

    Use

    Lentiviral

    Tags

    IRES-GFP

    Expression

    Mutation

    M173, F234A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pF(UG) hSyn SYP-deltaTMD SYT7alpha

    Plasmid

    #179718

    Purpose

    Lentiviral expression of synaptic vesicle-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag

    Plasmid

    #179719

    Purpose

    Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pF(UG) hSyn PM-SYT7alpha deltaTMD-HaloTag

    Plasmid

    #179721

    Purpose

    Lentiviral expression of plasma membrane-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD

    Plasmid

    #179722

    Purpose

    Lentiviral expression of lysosome-targeted mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pF(UG) hSyn Lamp1-SYT7alpha deltaTMD-HaloTag

    Plasmid

    #179723

    Purpose

    Lentiviral expression of lysosome-targeted Halo tagged mouse Syt7

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    SYT7 (Syt7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pF(UG) U6-SYT7 sgRNA 777 HaloTag

    Plasmid

    #179724

    Purpose

    U6-driven SYT7 gRNA and HaloTag lentiviral vector for CRISPR/HITI

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    sgRNA and HaloTag
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pF(UG) CamKII sf iGluSnFR S72A

    Plasmid

    #179726

    Purpose

    Lentiviral expression of iGluSnFR biosensor for monitoring synaptic vesicle exocytosis and glutamate release

    Depositor

    Edwin Chapman

    Article

    Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

    Insert

    iGluSnFR
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    S72A

    Promoter

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pPGK-SB13

    Plasmid

    #236078

    Purpose

    SB13 transposase, for use with Sleeping Beauty transponson plasmids

    Depositor

    Masashi Narita

    Article

    Chan et al Nature. 2024 Sep;633(8030):678-685. doi:

    Insert

    SB13 transposase
    

    Use

    Sleeping beauty (sb) transposase plasmid

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. AAV-GfaABC1D-ERCreER-4x6T

    Plasmid

    #196411

    Purpose

    Astrocytic expression of ERT2-Cre-ERT2 recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Insert

    ERT2-Cre-ERT2
    

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pETDuet1_6xHis-TEV-Lambda-Protein-Phosphatase

    Plasmid

    #223747

    Purpose

    Expression of recombinant protein for purification

    Depositor

    Sascha Martens

    Article

    Adriaenssens et al Nat Cell Biol. 2025 Jul 25. doi: 10.1038

    Insert

    Lambda-PPase
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pAAV-Syn_CapChR2_mScarlet

    Plasmid

    #188039

    Purpose

    Expresses CapChR2-mScarlet in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-mScarlet
    

    Use

    AAV

    Tags

    mScarlet

    Expression

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    hSyn

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCOLA-Gent-EM7-Erv1p-PDI

    Plasmid

    #202486

    Purpose

    Expresses sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) constitutively

    Depositor

    Michael Lynch

    Article

    Hennigan et al Metab Eng. 2024 Sep;85:116-130. doi: 10.

    Insert

    sulfhydryl oxidase (Erv1p) and protein disulfide bond isomerase (PDI) (ERV1 Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    EM7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p-eGFP-beta 2 CP

    Plasmid

    #13298

    Depositor

    John Cooper

    Article

    Schafer et al J Cell Biol. 1998 Dec 28. 143(7):1919-30

    Insert

    capping protein beta 2 subunit (Capzb Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pLL5.0 E-cadherin shRNA/Ecad-tdTomato

    Plasmid

    #101278

    Purpose

    Lentiviral expression of shRNA targeting Ecad and Ecad-tdtomato

    Depositor

    Alpha Yap

    Article

    Wu et al Nat Cell Biol. 2014 Feb;16(2):167-78. do

    Insert

    E-cadherin
    

    Use

    Lentiviral

    Tags

    tdtomato

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. AAV-GfaABC1D-Cre-4x6T

    Plasmid

    #196410

    Purpose

    Astrocytic expression of Cre recombinase in AAV; astrocyte specificity enhanced with 4x6T miRNA targeting cassette

    Depositor

    Stanley Thomas Carmichael

    Article

    Gleichman et al Nat Commun. 2023 Nov 16;14(1):7426. doi:

    Has Service

    AAV5

    Insert

    Cre
    

    Use

    AAV; Astrocyte-selective

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and GfaABC1D

    Available Since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pCMVTNT-T7-KPNA2

    Plasmid

    #26678

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 2 (KPNA2 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pCMVTNT-T7-KPNA3

    Plasmid

    #26679

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 3 (KPNA3 Human)
    

    Use

    Tags

    T7 (MASMTGGQQMG)

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 24, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pEHIShqprt

    Plasmid

    #92166

    Purpose

    Plasmid pEHIShqprt expressing the full-length of human quinolinate phosphoribosyltransferase (hQPRTase) was constrcucted by inserting BspHI/BamHI-fragment into NcoI/BamHI digested pEHISTEV vector

    Depositor

    Huanting Liu, James Naismith

    Article

    Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

    Insert

    Quinolinate Phosphoribosyltransferase (QPRT Human)
    

    Use

    Tags

    TEV protease -cleavable N-terminal 6His

    Expression

    Bacterial

    Mutation

    changed Asp 2 to Asn to create BspHI site

    Promoter

    T7

    Available Since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pCMVTNT-T7-KPNA1

    Plasmid

    #26677

    Depositor

    Bryce Paschal

    Article

    Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

    Insert

    Karyopherin Alpha 1 (KPNA1 Human)
    

    Use

    Tags

    T7

    Expression

    Mammalian

    Mutation

    KPNA1 sequence contains a reported SNP at S73N

    Promoter

    Available Since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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47. pRG/III-hs-MBP-CB2-HF

    Plasmid

    #121682

    Purpose

    Bacterial expression of cannabinoid receptor CB2 fusion protein

    Depositor

    Richard Henderson, Reinhard Grisshammer

    Article

    Calandra et al Biotechnology Letters

    Insert

    peripheral cannabinoid receptor (CNR2 Human)
    

    Use

    Tags

    His6 Flag tag

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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48. pBBR15.2-2H12.D11opt-scarRef

    Plasmid

    #221150

    Purpose

    cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Inserts

    cdGreen2.1
    mScarlet-I
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    N-terminus changed: starts with MSKKYGEAVIKE ...

    Promoter

    Constitutive and None (same as cdGreen2.1)

    Available Since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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49. pHJ12: CadC-VHH-Caffeine (No linker)

    Plasmid

    #108244

    Purpose

    Membrane bound transcription activator CadC fusing VHH-Caffeine. Upon ligand binding, CadC-VHH-Caffeine can be dimerized and activate down stream reporter gene sfGFP expression

    Depositor

    Jerome Bonnet

    Article

    Chang et al ACS Synth Biol. 2017 Oct 30. doi: 10.102

    Insert

    Membrane bound transcription activator CadC fusing with VHH-Caffeine antibody without external linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    pLacO1

    Available Since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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50. pAAV-Syn_CapChR2_eYFP

    Plasmid

    #188035

    Purpose

    Expresses CapChR2-eYFP in mammalian neurons

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR2-eYFP
    

    Use

    AAV

    Tags

    eYFP

    Expression

    Mutation

    S43D, L112C, T139C and N238E mutations in CoChR

    Promoter

    hSyn

    Available Since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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