We narrowed to 19,954 results for: Kin

1. pCS2 GFP-GSK3mut-MAPK

   Plasmid

   #29690

   Purpose

   GFP reporter protein fused to three mutated GSK3 phosphorylation sites

   Depositor

   Edward De Robertis

   Article

   Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

   Insert

   GFP glycogen synthase kinase 3 recognition site mutated primed by MAPK
   

   Use

   Tags

   Flag and Flag-EGFP

   Expression

   Mammalian

   Mutation

   recognition sites only, and E3 ligase site, GSK3 …

   Promoter

   SP6

   Available Since

   Jan. 6, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. 9X NFAT-apha-MHC-Luc

   Plasmid

   #51941

   Purpose

   9 copies of NFAT sites in minimal alphaMHC-pGL-3

   Depositor

   Jeffery Molkentin

   Article

   Wilkins et al Circ Res. 2004 Jan 9;94(1):110-8. Epub 2

   Inserts

   9x NFAT binding site
   alpha-MHC
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   Promoter

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28_human-pro-IL18

   Plasmid

   #183390

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA5 FRT/TO Plk4 ∆24-mCherry

   Plasmid

   #80271

   Purpose

   mammalian expression plasmid for Plk4 with multi-phospho degron deleted

   Depositor

   Andrew Holland

   Article

   Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

   Insert

   Plk4 (PLK4 Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deletion of multi-phospho degron (amino acids 282…

   Promoter

   CMV

   Available Since

   Aug. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a_Pro-IL-1α

   Plasmid

   #214341

   Purpose

   Protein overexpression in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL1A (IL1A Human)
   

   Use

   Tags

   His-TEV and Myc

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCellFree_G03 CIRBP

   Plasmid

   #67055

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   CIRBP (CIRBP Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Expression

   Mutation

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pFastBac1 Flag BRG1

   Plasmid

   #1957

   Depositor

   Robert Kingston

   Article

   Phelan et al Mol Cell 1999 Feb;3(2):247-53.

   Insert

   BRG1 (SMARCA4 Human)
   

   Use

   Tags

   flag

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 18, 2006

   Availability

   Academic Institutions and Nonprofits only

8. GFP-PIPK1 gamma 87

   Plasmid

   #22300

   Depositor

   Pietro De Camilli

   Article

   Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

   Insert

   human phosphatidylinositol phosphate kinase type 1 gamma 87 (PIP5K1C Human)
   

   Use

   Tags

   GFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 10, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONR221_SLC22A2

   Plasmid

   #131964

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Mocking et al Int J Mol Sci. 2022 Jan 21;23(3):1203. d

   Insert

   SLC22A2 (SLC22A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 21, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pcDNA3 MEKK1 KD

    Plasmid

    #12180

    Depositor

    Gary Johnson

    Article

    Widmann et al Mol Cell Biol. 1998 Apr . 18(4):2416-29.

    Insert

    MEKK1 (Map3k1 Mouse)
    

    Use

    Tags

    His and Xpress

    Expression

    Mammalian

    Mutation

    Kinase dead: K1253M; V1467L

    Promoter

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pCIG2-Flag-mNuak1 WT

    Plasmid

    #168507

    Purpose

    For expression of Nuak1 (AMPK-related kinase also called ARK5/Omphk1)

    Depositor

    Franck Polleux

    Article

    Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

    Insert

    Nuak1 (Nuak1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-human-pro-IL-18

    Plasmid

    #214317

    Purpose

    Expression of gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myc

    Expression

    Mutation

    Promoter

    MESV

    Available Since

    Feb. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBABE HSF Flag wt

    Plasmid

    #1948

    Depositor

    Robert Kingston

    Article

    Sullivan et al Mol Cell Biol 2001 Sep;21(17):5826-37.

    Insert

    Heat Shock Factor 1 (HSF1 Human)
    

    Use

    Retroviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 23, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR-PKAKTRClover

    Plasmid

    #59146

    Purpose

    ORF encoding PKA KTR mClover flanked by gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    PKA Kinase Translocation Reporter (PRKACA Mouse, Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 15, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLHCX-Flag-mPKM2

    Plasmid

    #42512

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-FKBP:mCherry-Rab7a

    Plasmid

    #72903

    Purpose

    Late endosome-targeted mCherry-tagged recruiter construct

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Rab7a (RAB7A Human)
    

    Use

    Lentiviral

    Tags

    FKBP, mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 1127-DP NTP wild type

    Plasmid

    #32398

    Depositor

    Kathleen Green

    Article

    Kowalczyk et al J Cell Biol. 1997 Nov 3;139(3):773-84.

    Insert

    Desmoplakin (DSP Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Contains only the first 584 aa

    Promoter

    CMV

    Available Since

    Nov. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

18. mEos2-Mito-7

    Plasmid

    #57401

    Purpose

    Localization: Mitochondria, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Mito (COX8A Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pHR-FKBP:mCherry-Rab11a

    Plasmid

    #72902

    Purpose

    Recycling endosome-targeted mCherry-tagged recruiter construct

    Depositor

    Ivan Yudushkin

    Article

    Ebner et al J Cell Biol. 2017 Jan 31. pii: jcb.20161

    Insert

    Rab11a (RAB11A Human)
    

    Use

    Lentiviral

    Tags

    FKBP and mCherry

    Expression

    Mammalian

    Mutation

    fragment a.a. 2-216

    Promoter

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRelA'

    Plasmid

    #175595

    Purpose

    Tet inducible, KAN Resistant, non labelled synthesis domain of the E. coli relA gene on a low copy backbone. Used to induce controllable synthesis of ppGpp in E. coli.

    Depositor

    Greg Bokinsky

    Article

    Buke et al Curr Biol. 2022 Feb 28;32(4):870-877.e5.

    Insert

    Catalytic domain of E.coli relA gene.
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Only the catalytic domain of the enzyme is used (…

    Promoter

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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