We narrowed to 17,568 results for: bon;

1. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

   Plasmid

   #132560

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop replaced by com aptamer
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-SLC35B2-sgRNA

   Plasmid

   #154860

   Purpose

   Lentiviral expression of Cas9 and sgRNA targeting SLC35B2

   Depositor

   Yihong Ye

   Article

   Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

   Insert

   SLC35B2 sgRNA (SLC35B2 Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAV11K

   Plasmid

   #63184

   Purpose

   pRS416 backbone, URA3 replaced by KanMX, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for transcription unit (TU) assembly using yGG.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Sept. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSicoR-Ef1a-mCh-Puro-GFPi

   Plasmid

   #31848

   Depositor

   Bruce Conklin

   Article

   Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

   Insert

   GFP RNAi
   

   Use

   Cre/Lox, Lentiviral, and RNAi

   Expression

   Mammalian

   Promoter

   U6 for shRNA; Ef1alpha for mCherry-puromycin

   Available Since

   Jan. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR8/GW/TOPO-SRF

   Plasmid

   #98618

   Purpose

   Gateway entry TOPO TA cloning vector for SRF

   Depositor

   Richard Harvey

   Article

   Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

   Insert

   SRF (Srf Mouse)
   

   Use

   Topo ta cloning vector

   Available Since

   July 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAFMW-Nbr-D435A/E437A

   Plasmid

   #40829

   Purpose

   Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

   Depositor

   Phillip Zamore

   Article

   Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

   Insert

   Nbr D435A/E437A (Nbr Fly)
   

   Tags

   3xFLAG and 6xMyc

   Expression

   Insect

   Mutation

   D435A/E437A, part of the DEDD motif; mutations p…

   Promoter

   Actin5C

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

7. pAV10.K5

   Plasmid

   #63210

   Purpose

   pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into YKL162C-A (NotI or BciVI).

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAV10.K4

   Plasmid

   #63212

   Purpose

   pCACS backbone (Amp), TRP1, contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. Integration into chrIXR: 387328-388330.

   Depositor

   Jef Boeke

   Article

   Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.

   Type

   Empty backbone

   Use

   Synthetic Biology

   Expression

   Bacterial and Yeast

   Available Since

   Feb. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCfB2903(XI-2 MarkerFree)

   Plasmid

   #73275

   Purpose

   EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-2 (Chr XI: 91575..92913)

   Depositor

   Irina Borodina

   Article

   Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Expression

   Yeast

   Available Since

   Nov. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMY-MCS-P2A-BlastR-DYK

    Plasmid

    #163357

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated Blasticidin selection marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCML 961

    Plasmid

    #98848

    Purpose

    pTriFC_aidB. Expresses protein-NYFP and 2MS2BD-5' UTR mStrawberry fusions in E. coli for Dual-fluorescence TriFC assay (ampR).

    Depositor

    Lydia Contreras

    Article

    Sowa et al Nucleic Acids Res. 2017 Feb 28;45(4):167

    Inserts

    5' UTR + first 100 nucleotides of coding sequence of the aidB gene
    csrA
    

    Expression

    Bacterial

    Promoter

    pLacO

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcrF2

    Plasmid

    #89234

    Purpose

    Plasmid contains the gene for anti-CRISPR protein AcrF2 (gene 30 from bacteriophage D3112), with N-terminal 6his tag and TEV protease cleavage site

    Depositor

    Blake Wiedenheft

    Article

    Chowdhury et al Cell. 2017 Mar 23;169(1):47-57.e11. doi:

    Insert

    AcrF2
    

    Tags

    6xHis-TEV

    Available Since

    April 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEA02

    Plasmid

    #172193

    Purpose

    Suicide vector carrying the site attL of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.

    Depositor

    Jean-Luc Pernodet

    Article

    Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

    Insert

    attL
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEA03

    Plasmid

    #172194

    Purpose

    Suicide vector carrying the site attR of the SSR system of pSAM2. This plasmid is integrated into the genome of Actinobacteria by HR when carrying a DNA fragment identical to a region of the genome.

    Depositor

    Jean-Luc Pernodet

    Article

    Santos et al Microorganisms. 2022 Apr 16;10(4). pii:

    Insert

    attR
    

    Expression

    Bacterial

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCfB3039(XII-2 MarkerFree)

    Plasmid

    #73279

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-2 (Chr XII: 808805..809939)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Yeast

    Available Since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. JP705: pMVP/SB/Neo-DEST

    Plasmid

    #121866

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Neo selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMP71-mtGFP-llo-lama4-alg8

    Plasmid

    #174605

    Purpose

    expresses palmytoylated GFP with a C terminal fusion of the LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 and Lama4

    Depositor

    Stanley Riddell

    Article

    Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

    Insert

    palmytoylGFP w/C-term fusion LLO190 and minigenes of 2 neoantigens from the T3 tumor in genes Alg8 Lama4
    

    Expression

    Mammalian

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Frt-hspB10

    Plasmid

    #63101

    Purpose

    Expression of HSPB10 (small HSP) in mammalian cells

    Depositor

    Harm Kampinga

    Article

    Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

    Insert

    hspb10 (ODF1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMY-MCS-P2A-CD90.1

    Plasmid

    #163354

    Purpose

    Retroviral backbone for in-frame cloning of a gene of interest and a P2A separated CD90.1 surface marker

    Depositor

    Teunis Geijtenbeek, Louis Ates

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. KK001: pMVP/SB/Hygro-DEST

    Plasmid

    #121867

    Purpose

    pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Sleeping beauty transposon, pmvp gateway destinat…

    Expression

    Mammalian

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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