4,180 results

1. pcDNA3-Casp8 C360A

   Plasmid

   #11818

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

   Insert

   Caspase 8 catalytic mutant (CASP8 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C360A

   Available Since

   May 12, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-E2F4

   Plasmid

   #10914

   Depositor

   Bob Weinberg

   Article

   Sardet et al Proc Natl Acad Sci U S A. 1995 Mar 14. 9

   Insert

   E2F4 (E2F4 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

3. pWPT-PDX1

   Plasmid

   #12256

   Depositor

   Didier Trono

   Article

   Ritz-Laser et al Diabetologia. 2003 Jun . 46(6):810-21.

   Insert

   Pdx1 (PDX1 Human)
   

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBiFC-CrN173

   Plasmid

   #22016

   Depositor

   Chang-Deng Hu

   Article

   Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

   Insert

   CrN173
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   N/A

   Available Since

   Sept. 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pU6-(BbsI)_CBh-Cas9-T2A-BFP-P2A-Ad4E1B

   Plasmid

   #64218

   Purpose

   Expression vector for sgRNA and for Expression of Cas9 linked to BFP via T2A linked to Ad4 E1B via P2A

   Depositor

   Ralf Kuehn

   Article

   Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

   Inserts

   Cas9
   sgRNA cassette
   

   Use

   CRISPR

   Tags

   3xFLAG, NLS, and T2A-BFP-P2A-E1B

   Expression

   Mammalian

   Promoter

   CBh and U6

   Available Since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCherry-mNrp1

   Plasmid

   #21934

   Depositor

   Guido Serini

   Article

   Valdembri et al PLoS Biol. 2009 Jan 27. 7(1):e25.

   Insert

   neuropilin 1 (Nrp1 Mouse)
   

   Tags

   Cherry and His

   Expression

   Mammalian

   Available Since

   Oct. 13, 2009

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3.1 myc TSC1

   Plasmid

   #12133

   Depositor

   Cheryl Walker

   Article

   Cai et al J Cell Biol. 2006 Apr 24. 173(2):279-89.

   Insert

   TSC1 (TSC1 Human)
   

   Tags

   his and myc

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. YFP-tagged FRB (YR)

   Plasmid

   #20148

   Depositor

   Tobias Meyer

   Article

   Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

   Insert

   YFP-FRB (YR)
   

   Expression

   Mammalian

   Available Since

   March 31, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-TET-MKOS

   Plasmid

   #20959

   Purpose

   piggyBac vector with tet inducible cMyc, KLF4, Oct4, Sox2 for creation of induced pluripotent stem cells

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   cMyc, KLF4, Oct4, Sox2 (Myc, Klf4, Pou5f1, Sox2 Mouse)
   

   Use

   piggybac

   Tags

   IRES-bGeo

   Expression

   Mammalian

   Available Since

   April 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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10. FUW-tetO-hKLF4

    Plasmid

    #20725

    Depositor

    Rudolf Jaenisch

    Article

    Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

    Insert

    KLF4 (KLF4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. 1066 pBabe puroL PTEN

    Plasmid

    #10785

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Nov. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMJ825

    Plasmid

    #39315

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Mutation

    Aspartate 10 to Alanine (D10A)

    Promoter

    T7

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. FUW-SOKM

    Plasmid

    #20325

    Purpose

    Lentiviral plasmid expressing mouse Sox2, Oct4, Klf4 and cMyc for iPS cell generation

    Depositor

    Rudolf Jaenisch

    Article

    Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

    Insert

    Sox2-P2A-Oct4-T2A-Klf4-E2A-cMyc (Pou5f1, Sox2, Klf4, Myc Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-ABE7.9

    Plasmid

    #102918

    Purpose

    expresses ABE7.9 in mammalian cells

    Depositor

    David Liu

    Article

    Gaudelli et al Nature. 2017 Nov 23;551(7681):464-471. d

    Insert

    ABE7.9
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV-pgk-Cre

    Plasmid

    #24593

    Depositor

    Patrick Aebischer

    Has Service

    AAV Retrograde

    Insert

    Cre recombinase (codon optimized)
    

    Use

    AAV and Cre/Lox; Adeno-associated

    Promoter

    pgk

    Available Since

    May 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2+hSpCas9

    Plasmid

    #51815

    Purpose

    Encode hSpCas9 for in vitro transcription with Sp6

    Depositor

    Masato Kinoshita

    Article

    Ansai et al Biol Open. 2014 Apr 11;3(5):362-71. doi:

    Insert

    humanized S.pyogenes Cas9
    

    Use

    CRISPR; Medaka, zebrafish

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    SP6

    Available Since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. EGFR (del3) L747-E749del, A750P

    Plasmid

    #11015

    Purpose

    Retroviral construct for expressing human EGFR with L747-E749 deletion and A750P mutation in human cells

    Depositor

    Matthew Meyerson

    Article

    Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

    Insert

    EGFR (del3) (EGFR Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    L747-E749 deleted, A750P

    Available Since

    Dec. 6, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pU6-(BbsI)_CBh-Cas9-T2A-mcherry-P2A-Ad4E4orf6

    Plasmid

    #64222

    Purpose

    Expression vector for sgRNA and for Expression of Cas9 linked via T2A to mCherry linked to the Ad4 E4orf6 gene via P2A

    Depositor

    Ralf Kuehn

    Article

    Chu et al Nat Biotechnol. 2015 Mar 24. doi: 10.103

    Inserts

    Cas9
    sgRNA cassette
    

    Use

    CRISPR

    Tags

    3xFLAG, NLS, and T2A-mCherry-P2A-E4orf6

    Expression

    Mammalian

    Promoter

    CBh and U6

    Available Since

    May 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. APEX2 - Actin in pEGFP

    Plasmid

    #66172

    Purpose

    APEX2 fused to the N-terminus of human beta actin

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    APEX2 - human beta Actin (ACTB Human, Synthetic)
    

    Tags

    Flag epitope tag (DYKDDDDK)

    Expression

    Mammalian

    Mutation

    APEX2 contains 4 mutations relative to wt soybean…

    Promoter

    CMV

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCFJ356 - MCS(cxTi10816, IV)

    Plasmid

    #34871

    Depositor

    Erik Jorgensen

    Article

    Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

    Insert

    cxTi10816 targeting
    

    Use

    Worm targeting

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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