We narrowed to 7,520 results for: Ski;

1. SiC-V2-Cas9G7

   Plasmid

   #133043

   Purpose

   Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

   Depositor

   Sriram Neelamegham

   Article

   Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

   Insert

   Tet repressor
   

   Use

   Lentiviral

   Tags

   Cerulean

   Available Since

   Nov. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. SNAP-OMP25

   Plasmid

   #141149

   Purpose

   Fluorescent labeling of the outer mitochondrial membrane (OMM) in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

   Insert

   OMP25 (Synj2bp Rat, Budding Yeast)
   

   Tags

   SNAP tag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDF0229 DisCas7-11 bacterial expression vector with golden gate site for guides

   Plasmid

   #172506

   Purpose

   Encodes DisCas7-11 protein alone with a golden gate site for spacer cloning

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Ozcan et al Nature. 2021 Sep 6. pii: 10.1038/s41586-

   Insert

   DisCas7-11 bacterial expression vector with golden gate site for guides
   

   Expression

   Bacterial

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA5/FRT_CMV_NES-Caprola_04-mEGFP

   Plasmid

   #194682

   Purpose

   CMV driven expression of the calcium recorder Caprola_04 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_04-mEGFP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTagRFP658-N1

   Plasmid

   #178974

   Purpose

   Expresses TagRFP658 in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

   Insert

   TagRFP658
   

   Tags

   no

   Expression

   Mammalian

   Mutation

   NO

   Promoter

   CMV

   Available Since

   Aug. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3 Ash2L full

   Plasmid

   #15548

   Depositor

   Kai Ge

   Article

   Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

   Insert

   Ash2L (ASH2L Human)
   

   Expression

   Mammalian

   Mutation

   R622H (Please see depositor comments below)

   Available Since

   Aug. 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA5/FRT_CMV_NES-Caprola_05-mEGFP

   Plasmid

   #194683

   Purpose

   CMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFP

   Depositor

   Kai Johnsson

   Article

   Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

   Insert

   Caprola_05-mEGFP
   

   Tags

   mEGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-R-iLACCO1

   Plasmid

   #208025

   Purpose

   Biosensor for intracellular L-lactate

   Depositor

   Robert Campbell

   Article

   Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

   Insert

   R-iLACCO1
   

   Expression

   Mammalian

   Available Since

   Oct. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLYS6-METTL17-FLAG

   Plasmid

   #220875

   Purpose

   Expressing METTL17-FLAG

   Depositor

   Vamsi Mootha

   Article

   Ast et al Mol Cell. 2024 Jan 18;84(2):359-374.e8.

   Insert

   METTL17 (METTL17 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCEFL EGFP-TEADi

    Plasmid

    #140144

    Purpose

    TEAD inhibitor (TEADi): GFP-tagged inhibitor of the interaction of YAP1 and TAZ with TEAD transcription factors with nuclear localization signal.

    Depositor

    Ramiro Iglesias-Bartolome

    Article

    Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

    Insert

    EGFP TEADi
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    April 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. FLAG-p54

    Plasmid

    #35379

    Depositor

    Benjamin Blencowe

    Article

    Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

    Insert

    p54 (NONO Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    A464V

    Promoter

    CMV

    Available Since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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12. SNAP-Sec61B

    Plasmid

    #141152

    Purpose

    Fluorescent labeling of ER membrane in mammalian cells

    Depositor

    Gia Voeltz

    Article

    Abrisch et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    SNAP tag

    Expression

    Mammalian

    Mutation

    L79M on Sec61B

    Promoter

    CMV

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEND-320_Cacnes_ori

    Plasmid

    #225602

    Purpose

    Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a low-copy pBR322 E. coli ori

    Depositor

    Marc Güell

    Article

    Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Feb. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSLIK-Rbfox2-rABE-Flag

    Plasmid

    #191384

    Purpose

    Doxycycline inducible expression of Rbfox2-rABE

    Depositor

    Stephen Floor

    Article

    Lin et al Nat Methods. 2023 Dec;20(12):1887-1899.

    Insert

    Rbfox2-rABE (RBFOX2 Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Promoter

    tet-inducible promoter

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAIP hIL2 co

    Plasmid

    #90513

    Purpose

    Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    IL2 (IL2 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Adeno EA

    Plasmid

    #64071

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 19 of murine Alk and intron 14 of Eml4

    Depositor

    Andrea Ventura

    Article

    Maddalo et al Nature. 2014 Dec 18;516(7531):423-7. doi

    Insert

    U6_sgRNA(Alk)_U6_sgRNA(Eml4)_CBh_FLAG-Cas9
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Promoter

    U6 and CBh

    Available Since

    July 23, 2015

    Availability

    Academic Institutions and Nonprofits only

17. pInducer20 Cyclin E1

    Plasmid

    #109348

    Purpose

    virus packaged, doxycyline inducible expression of Cyclin E1

    Depositor

    Jean Cook

    Article

    Matson et al Elife. 2017 Nov 17;6. doi: 10.7554/eLife

    Insert

    Cyclin E1 (CCNE1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA pDEST40 Cas9-POLD3

    Plasmid

    #183194

    Purpose

    Expression clone

    Depositor

    Emma Haapaniemi

    Article

    Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

    Insert

    POLD3
    

    Use

    CRISPR

    Tags

    Cas9WT

    Expression

    Mammalian

    Mutation

    n/a

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-hysn-flex-dsRed-shscramble

    Plasmid

    #71383

    Purpose

    Expresses scramble shRNA Cre-dependently by flex switch

    Depositor

    William Wisden

    Article

    Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

    Insert

    human synapsin promoter-flex switch-dsRed-shscramble
    

    Use

    AAV, Cre/Lox, and RNAi

    Expression

    Mammalian

    Promoter

    human synapsin

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

    Plasmid

    #169333

    Purpose

    Expression of HaloTag9 at the mitochondrial surface of mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    TOMM20-HaloTag9 (TOMM20 Human)
    

    Tags

    HaloTag9

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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