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p2T-CMV-BE4max-BlastR

Plasmid

#152991

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

BE4-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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TB:7xSMAD_RE:MiniP

Plasmid

#133879

Purpose

7 copies the SMAD DNA binding motif

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Curr Protoc Mol Biol. 2020 Jun;131(1):e1

Insert

7 copies the SMAD DNA binding motif

Use

Luciferase and Synthetic Biology

Expression

Mammalian

Available Since

June 17, 2020

Availability

Academic Institutions and Nonprofits only

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pFlare5GdP

Plasmid

#90250

Purpose

Minigene reporter for alternative splicing analysis

Depositor

Douglas Black

Article

Zheng et al Genome Res. 2013 Jun;23(6):998-1007. doi

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSB161 - pL2_pSB90_2x35S::GUS::tMAS

Plasmid

#123197

Purpose

binary plant vector for transient expression of GUS (with introns)

Depositor

Erin Cram, Carolyn Lee-Parsons

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

2x35S::GUS::tMAS

Expression

Plant

Available Since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-Z22

Plasmid

#120065

Purpose

Template for C-terminal PCR tagging of mammalian genes (Zeocin selection) with SNAP

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

SNAP

Use

Pcr template

Tags

SNAP

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-P15

Plasmid

#120026

Purpose

Template for C-terminal PCR tagging of mammalian genes (Puromycin selection) with miRFP670

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

miRFP670

Use

Pcr template

Tags

miRFP670

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pT2K-CAGGS-U6-sgRNA-M5-U6-sgRNA-M7-U6-sgRNA-M9-IRES-CFP

Plasmid

#114729

Purpose

3 sgRNAs targeting a sequence upstream of the initiator ATG of the cellular Myc gene

Depositor

Martine Roussel

Article

Vo et al Sci Rep. 2018 Jun 7;8(1):8733. doi: 10.1

Insert

sgRNA M5, sgRNA M7, sgRNA M9

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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Kohinoor-Clathrin

Plasmid

#67773

Purpose

Clathrin-targetted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor

Tags

Clathrin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
Kohinoor-Vimentin/pcDNA3

Plasmid

#67772

Purpose

Vimentin-targeted Kohinoor for mammalian cell expression

Depositor

Takeharu Nagai

Article

Tiwari et al Nat Methods. 2015 Jun;12(6):515-8. doi:

Insert

Kohinoor-Vimentin

Tags

Vimentin

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2015

Availability

Academic Institutions and Nonprofits only
pTRE2-Hyg-omMx1

Plasmid

#52420

Purpose

Inducible expression (Tet-Off system) of the rainbow trout antiviral Mx1 protein

Depositor

Bertrand Collet

Article

Lester et al J Virol Methods. 2012 Jun;182(1-2):1-8.

Insert

Oncorhynchus mykiss interferon-induced GTP-binding protein Mx1 (mx1)

Expression

Mammalian

Promoter

TRE2

Available Since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV10-Flag-Fbxl3

Plasmid

#48751

Purpose

Expression of FLAG-tagged Fbxl3

Depositor

Joseph Takahashi

Article

Siepka et al Cell. 2007 Jun 1;129(5):1011-23. Epub 20

Insert

Fbxl3 (Fbxl3 Mouse)

Tags

3XFLAG

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
p_sc-eVIP

Plasmid

#168174

Purpose

Expresses barcoded coding genetic variants in mammalian cells

Depositor

Jesse Boehm, James T. Neal, Aviv Regev

Article

Ursu et al Nat Biotechnol. 2022 Jun;40(6):896-905.

Insert

TP53 (TP53 Human)

Use

Lentiviral

Tags

N/A

Expression

Mammalian

Mutation

Wildtype

Promoter

EF-1a

Available Since

Jan. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pFRLuc-CoV2-WT

Plasmid

#177614

Purpose

Dual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting

Depositor

Junjie Guo

Article

Sun et al Proc Natl Acad Sci U S A. 2021 Jun 29;11

Insert

SARS-CoV-2 FSE

Use

Luciferase

Expression

Mammalian

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

Plasmid

#170320

Purpose

Expresses HA and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Mutation

None

Available Since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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SPACE-VRQR (pRZ5137)

Plasmid

#140245

Purpose

CMV promoter expression plasmid for TadA*(V82G)-nCas9_VRQR-pmCDA1(R187W)-UGI-UGI-P2A-EGFP

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

SPACE_VRQR

Expression

Mammalian

Mutation

V82G in TadA*, VRQR mutations in SpCas9, D10A in …

Promoter

CMV

Available Since

June 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-ACBEmax

Plasmid

#139106

Purpose

Mammalian ACBEmax expression vector (pSI-632)

Depositor

Nozomu Yachie

Article

Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS

Use

CRISPR

Expression

Mammalian

Promoter

CMV promoter

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAOJ15

Plasmid

#91567

Purpose

Broad host range allelic exchange suicide vector for bacteria - Tse2 toxin driven by anhydrotetracycline inducible promoter

Depositor

Harry Mobley

Article

Armbruster et al PLoS Pathog. 2017 Jun 14;13(6):e1006434.

Type

Empty backbone

Expression

Bacterial

Promoter

Tetracycline promoter

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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p3VEA3-3A

Plasmid

#234797

Purpose

Mammalian expression of bovine arrestin-3 (I385A,V386A,F387A)

Depositor

Vsevolod Gurevich

Article

Breitman et al J Biol Chem. 2012 Jun 1;287(23):19653-64

Insert

Arrestin-3 (ARR3 Bovine)

Tags

Venus

Expression

Mammalian

Mutation

I385A,V386A,F387A

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Kmt5cFL

Plasmid

#235581

Purpose

Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Kmt5c fused with scFV to programme H4K20me3

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Kmt5c (Kmt5c Mouse)

Use

CRISPR

Available Since

April 29, 2025

Availability

Academic Institutions and Nonprofits only

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SPDK3902(TRV-RNA2-pPEBV-MCS-AtGly-tRNA)

Plasmid

#149278

Purpose

Modified Tobacco rattle virus RNA2 vector that could be used for expression of sgRNAs

Depositor

Savithramma Dinesh-Kumar

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

Modified TRV RNA2 with PEBV promoter and AtGly-tRNA mobile RNA sequence

Use

T-dna vector

Mutation

T2318C (DNA) in PEBV coat protein sequence

Available Since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

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