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PHAGE-P CMVt N-HA hE6-AP I (G20V)

Plasmid

#119308

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (G20V) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

G20V

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (C21Y)

Plasmid

#119304

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (C21Y) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

C21Y

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 55-376)

Plasmid

#119313

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 55-376 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 55-376

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (R39H)

Plasmid

#119309

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (R39H) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

G20V

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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IH401: pMVP (L5-L4) CMV promoter

Plasmid

#121726

Purpose

pMVP L5-L4 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Gateway Cloning and Synthetic Biology

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KA1063_pPB-hCMV*1-cHA-IRESH2BmKO2

Plasmid

#124169

Purpose

piggyBac-based Dox-inducible expression

Depositor

Hans Schöler

Article

Adachi et al Cell Stem Cell. 2018 Aug 2;23(2):266-275

Type

Empty backbone

Expression

Mammalian

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh1750_S2F-IMCg_doxy-CMV_mChe-Cdc42E7

Plasmid

#118614

Purpose

expression of mChe-tagged cdc42E7 under doxy-inducible CMV promoter

Depositor

Marina Chekulaeva

Article

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

tetON CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (AA 55-852)

Plasmid

#119314

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I AA 55-852 in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

Only AA 55-852

Promoter

CMV

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD52-bglpA

Plasmid

#89704

Purpose

Human CD52 expression plasmid with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CD52-bglpA (CD52 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD5cmyc2-bglpA

Plasmid

#110645

Purpose

Expression plasmid for c-Myc-tag embedded into human CD52 with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CDcmyc2-bglpA

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP

Plasmid

#121509

Purpose

Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9).

Depositor

Guangping Gao

Article

Wang et al Nat Biotechnol. 2018 Oct;36(9):839-842.

Insert

sgFah.intron9

Use

AAV

Expression

Mammalian

Promoter

U6

Available Since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-myc-Sema4A RNAiR

Plasmid

#115172

Purpose

expresses RNAi-resistant Sema4A with N-terminal myc tag

Depositor

Suzanne Paradis

Article

McDermott et al Mol Cell Neurosci. 2018 Jul 4;92:50-66.

Insert

Sema4A (Sema4a Mouse)

Tags

myc

Expression

Mammalian

Mutation

Silent mutation between amino acids in ORF (401-4…

Promoter

CMV

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-TO-Casp-rastvTEV-teTVMV2

Plasmid

#116098

Purpose

Expresses a combination of proteases and their reporter

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Casp-rastvTEV-teTVMV2

Expression

Mammalian

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-TO-Cer-HO1-FlpIn

Plasmid

#116037

Purpose

Supporting plasmid for testing protease circuits

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cer-HO1

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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PB-CMV-Cit-tehc-DHFR

Plasmid

#116058

Purpose

Reporter plasmid for measuring protease activity

Depositor

Michael Elowitz

Article

Gao et al Science. 2018 Sep 21;361(6408):1252-1258

Insert

Cit-tehc-DHFR

Expression

Mammalian

Available Since

Oct. 11, 2018

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (delta554_557/PDZ)

Plasmid

#112803

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Jurkiewicz et al Biochim Biophys Acta. 2017 May;1864(5):7

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 554_557 deleted/PDZ

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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p3 FLAG_CMV14/OCTN2 (delta14_22/delta447_454)

Plasmid

#112802

Purpose

Expression of OCTN2 mutant

Depositor

Katarzyna Nalęcz

Article

Czeredys et al PLoS One. 2013 Dec 13;8(12):e82105. doi:

Insert

OCTN2/SLC22A5 (Slc22a5 Rat)

Tags

3 x Flag

Expression

Mammalian

Mutation

amino acids 14_22 and 447_454 deleted

Available Since

Aug. 23, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/SP S245X with 5'UTR

Plasmid

#92370

Purpose

expression of 245X spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

S245X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/M87 N184X

Plasmid

#92367

Purpose

expression of M87 N184X spastin in mammalian cells

Depositor

Peter Baas

Article

Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

Insert

spastin (SPAST Human)

Expression

Mammalian

Mutation

1-460 bp deleted, N184X

Promoter

CMV

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag/C448Y with 5'UTR

Plasmid

#89318

Purpose

expression of C448Y spastin M1 and M87 in mammalian cells

Depositor

Peter Baas

Article

Solowska et al J Neurosci. 2014 Jan 29;34(5):1856-67. d

Insert

spastin (SPAST Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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