4,180 results

1. pAC152-dual-dCas9VP64-sgExpression

   Plasmid

   #48238

   Purpose

   Dual expression construct expressing both dCas9VP64 and sgRNA from separate promoters

   Depositor

   Rudolf Jaenisch

   Article

   Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

   Insert

   dCas9
   

   Use

   CRISPR

   Tags

   HA Tag, HA-Tag, and VP64

   Expression

   Mammalian

   Mutation

   D10A;H840A

   Available Since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-myc-Atg5

   Plasmid

   #24922

   Depositor

   Toren Finkel

   Article

   Lee et al Proc Natl Acad Sci U S A. 2008 Mar 4. 10

   Insert

   Atg5 (ATG5 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   June 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMXs-Hu-L-Myc

   Plasmid

   #26022

   Depositor

   Shinya Yamanaka

   Article

   Nakagawa et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   L-Myc (MYCL Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBabe N-Ras 61K

   Plasmid

   #12543

   Depositor

   Channing Der

   Article

   Khosravi-Far et al Mol Cell Biol. 1996 Jul . 16(7):3923-33.

   Insert

   NRas 61K (NRAS Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   61K constitutively activated.

   Available Since

   Sept. 8, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR4-SNAPf (w878-1)

   Plasmid

   #29652

   Depositor

   Eric Campeau

   Type

   Empty backbone

   Use

   Entry vector

   Tags

   SNAPf

   Available Since

   May 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHsp70-Cas9

   Plasmid

   #45945

   Purpose

   The codon-optimized Cas9 nuclease under the control of the Drosophila hsp70 promoter used in Gratz, et al. (2013). Plasmid is low copy number.

   Depositor

   Kate O'Connor-Giles, Melissa Harrison, Jill Wildonger

   Article

   Gratz et al Genetics. 2013 May 24.

   Insert

   Codon optimized Cas9
   

   Use

   CRISPR

   Tags

   3x FLAG

   Mutation

   Silent change from C to A at bp 834 of insert

   Available Since

   July 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. p3868 HA-E6-AP

   Plasmid

   #8648

   Depositor

   Peter Howley

   Article

   Talis et al J Biol Chem 1998 Mar 13;273(11):6439-45.

   Insert

   E6-AP (UBE3A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-T7-hOsa2

   Plasmid

   #17987

   Depositor

   Naoko Tanese

   Article

   Inoue et al J Biol Chem. 2002 Nov 1. 277(44):41674-8

   Insert

   Osa2 (ARID1B Human)
   

   Tags

   T7 epitope

   Expression

   Mammalian

   Available Since

   Aug. 19, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. p1324 HPV-16 E7

   Plasmid

   #8643

   Depositor

   Peter Howley

   Article

   Münger et al J Virol. 1989 Oct . 63(10):4417-21.

   Insert

   HPV-16 E7 (E7 Human papillomavirus)
   

   Expression

   Mammalian

   Available Since

   June 16, 2005

   Availability

   Academic Institutions and Nonprofits only

10. pLOVE-Klf4

    Plasmid

    #15950

    Depositor

    Miguel Ramalho-Santos

    Article

    Blelloch et al Cell Stem Cell. 2007 Sep 13;1(3):245-7.

    Insert

    Kruppel-like factor 4 (Klf4 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    The recombination reaction required to clone &quo…

    Available Since

    Nov. 7, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHJ320

    Plasmid

    #20671

    Depositor

    Stephen Shaw

    Article

    Hao et al J Cell Biol. 2009 Feb 9. 184(3):451-62.

    Insert

    Moesin (MSN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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12. LeGO-iT2

    Plasmid

    #27343

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, IRES-tdTomato
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFP(C3)-Vinculin

    Plasmid

    #30312

    Depositor

    Klaus Hahn

    Insert

    Vinculin (Vcl Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    F633S, V828E, L936P in mouse viniculin (NM_009502…

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. pRK5-myc-RhoA-wt

    Plasmid

    #12962

    Depositor

    Gary Bokoch

    Insert

    RhoA (RHOA Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pH3U3-mcs

    Plasmid

    #12609

    Depositor

    Scot Wolfe

    Article

    Meng et al Nat Biotechnol. 2005 Aug . 23(8):988-94.

    Insert

    Multiple cloning site in His3 Ura3 reporter plasmid
    

    Expression

    Bacterial

    Available Since

    Sept. 26, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV-FLEX-rev-ChR2(H134R)-mCherry

    Plasmid

    #18916

    Purpose

    Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

    Depositor

    Scott Sternson

    Article

    Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    mCherry

    Mutation

    H134R

    Promoter

    CAG

    Available Since

    Jan. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. B23(NPM)

    Plasmid

    #34553

    Depositor

    Mary Dasso

    Article

    Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

    Insert

    B23/nucleophosmin (NPM1 Human)
    

    Tags

    DsRed

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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18. pmTurquoise2-NES

    Plasmid

    #36206

    Purpose

    In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

    Insert

    NES
    

    Tags

    mTurquoise2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 15, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Syn-Chronos-GFP

    Plasmid

    #59170

    Purpose

    AAV-mediated expression of Chronos-GFP under the Syn promoter

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Has Service

    AAV Retrograde, AAV1, and AAV5

    Insert

    Chronos-GFP
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    Syn

    Available Since

    Aug. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. tdTomato-Lifeact-7

    Plasmid

    #54528

    Purpose

    Localization: Actin, Excitation: 554, Emission: 581

    Depositor

    Michael Davidson

    Article

    Fiolka et al Proc Natl Acad Sci U S A. 2012 Apr 3;109

    Type

    Empty backbone

    Tags

    tdTomato-Lifeact

    Expression

    Mammalian

    Available Since

    June 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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