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MSCV Myc IRES GFP

Plasmid

#18770

Depositor

Scott Lowe

Publication

Hemann et al Nature. 2005 Aug 11. 436(7052):807-11.

Insert

Myc (Myc Mouse)

Use

Retroviral

Tags

IRES GFP

Expression

Mammalian

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles
pMevT

Plasmid

#17815

Depositor

Jay Keasling

Publication

Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGEM-Oct4

Plasmid

#16352

Depositor

James Thomson

Publication

Yu et al Science. 2007 Dec 21. 318(5858):1917-20.

Insert

Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1) (POU5F1 Human)

Expression

Bacterial

Available since

Jan. 4, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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LV-Lac

Plasmid

#12108

Depositor

Inder Verma

Publication

Pfeifer et al Proc Natl Acad Sci U S A. 2001 Sep 25. 9

Insert

LacZ

Use

Lentiviral

Expression

Mammalian

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pcDNA3-HA-14-3-3 beta

Plasmid

#13270

Depositor

Michael Yaffe

Insert

14-3-3 beta (YWHAB Human)

Tags

HA

Expression

Mammalian

Available since

Oct. 7, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pEGFP-NMHC II-C

Plasmid

#10843

Depositor

Robert Adelstein

Publication

Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

NMHC II-C (Myh14 Mouse)

Tags

EGFP

Expression

Mammalian

Available since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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His6-TEV-CRBN-midi

Plasmid

#215330

Purpose

CRBN midi construct for use in crystallography and biophysical studies enabling ligand and degrader design

Depositor

Alessio Ciulli

Publication

Kroupova et al Nat Commun. 2024 Oct 15;15(1):8885. doi:

Insert

CRBN (CRBN Human)

Tags

6xHis and TEV

Expression

Bacterial

Mutation

contains amino acids 41-187 followed by a Gly-Ser…

Promoter

lacI

Available since

March 26, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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P4-PE

Plasmid

#211375

Purpose

Packaging plasmid for v3b PE-eVLP production

Depositor

David Liu

Publication

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

P4-PE

Expression

Mammalian

Available since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-GAL9

Plasmid

#166689

Purpose

For creating a stable mCherry-GAL9 cell line

Depositor

Alan Sabirsh

Publication

Munson et al Commun Biol. 2021 Feb 16;4(1):211. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

LGALS9 (LGALS9 Human)

Use

Synthetic Biology

Tags

mCherry

Expression

Mammalian

Promoter

EF1a

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pET-pA-Hia5

Plasmid

#174372

Purpose

SEE DEPOSITOR COMMENTS BELOW. Plasmid for IPTG-inducible expression and purification of protein A-Hia5-6xHis (pA-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)

Depositor

Aaron Straight

Publication

Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi

Insert

protein A - hia5

Tags

6xHis and Protein A

Expression

Bacterial

Available since

Sept. 6, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRT043-CLYBL-DDdcas9-VPH

Plasmid

#158091

Purpose

Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

Depositor

Martin Kampmann

Publication

Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CLYBL-CAG-DDdCas9VPH-T2A-GFP

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLX_EFS-Cre_ppt-del

Plasmid

#162073

Purpose

Compact Cre expression vector with modified PPT designed to generate high-titer non-integrating lentivirus when packaged with PsPax2-D64V (Addgene #63586)

Depositor

Robert Manguso

Publication

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Insert

Cre

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EFS (part of insert)

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pmCherry-2xFYVE

Plasmid

#140050

Purpose

Expresses mCherry-tagged HRS 2xFYVE in mammalian cells

Depositor

Harald Stenmark

Publication

Sneeggen et al Nat Commun. 2019 Jun 28;10(1):2850. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

tandem HRS FYVE domain (Hgs Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

two FYVE domains (amino acids 147-223), linked by…

Promoter

CMV

Available since

May 3, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pDONR223 SARS-CoV-2 NSP16

Plasmid

#141269

Purpose

Gateway-compatible Entry vector, with insert of NSP16 gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1

Depositor

Fritz Roth

Publication

Kim et al G3 (Bethesda). 2020 Aug 6. pii: g3.120.4

Insert

NSP16 (ORF1ab SARS-CoV-2 isolate Wuhan-Hu-1)

Use

Gateway Cloning

Mutation

Many synonymous changes due to codon optimization

Available since

April 1, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

6 Articles

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HP17_KO7

Plasmid

#120346

Purpose

Helper plasmid for production of phagemid ssDNA, based on M13KO7

Depositor

Hendrik Dietz

Publication

Praetorius et al Nature. 2017 Dec 6;552(7683):84-87. doi:

Insert

Coding part of the M13KO7 helper phage genome

Expression

Bacterial

Promoter

native phage promoters

Available since

Aug. 30, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pET-32a(+)-NRG1B1

Plasmid

#120287

Purpose

Produce human recombinant NRG1 in DE3 cells

Depositor

Paul Burridge

Publication

Kuo et al Stem Cell Reports. 2020 Jan 9. pii: S221

Insert

NRG1-beta 1

Tags

6x His and S-Tag

Expression

Bacterial

Mutation

Codon optimized for E. Coli production

Promoter

T7 promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pFA6a-TurboID-3MYC-natMX6 (pFB1434)

Plasmid

#126050

Purpose

For C-terminal tagging with TurboID in yeast including nourseothricin-resistant marker

Depositor

François Bachand

Publication

Larochelle et al J Cell Sci. 2019 May 7. pii: jcs.232249.

Insert

TurboID

Use

Pcr-based c-terminal tagging

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pXL010-Wnt dual (GFP-Fire) reporter

Plasmid

#40588

Depositor

Sean Palecek

Publication

Azarin et al Biomaterials. 2012 Mar;33(7):2041-9. Epu

Insert

Wnt reporter sequences

Use

Lentiviral

Expression

Mammalian

Promoter

Wnt promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pKM464 (deletions)

Plasmid

#108322

Purpose

Orbit integrating plasmid to delete target gene.

Depositor

Kenan Murphy

Publication

Murphy et al MBio. 2018 Dec 11;9(6). pii: mBio.01467-

Insert

Bxb1 attB site

Use

Orbit integration plasmid for creating knockouts …

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3-ERK-SPARK

Plasmid

#106921

Purpose

Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

Depositor

Xiaokun Shu

Publication

Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

ERK-SPARK

Expression

Mammalian

Available since

March 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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