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Showing: 9751 - 9800 of 167210 results

  1. pLV Tmx1-V5

    Plasmid
    #175138
    Purpose
    Lentiviral expression of V5-tagged mouse Tmx1
    Depositor
    Larry Gerace
    Insert
    Tmx1 (Tmx1 Mouse)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    175138-plasmid-map-sequence-id-352052

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  2. pcDNA3.1(-)-flag-UBR2

    Plasmid
    #50573
    Purpose
    Expresses FLAG-tagged mouse UBR2 in mammalian cells.
    Depositor
    Alexander Varshavsky
    Insert
    UBR2 (Ubr2 Mouse)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 2, 2014
    Availability
    Academic Institutions and Nonprofits only

  4. pTCF7 HLA-B*07:02

    Plasmid
    #180446
    Purpose
    E. coli expression of soluble MHC protein
    Depositor
    John Altman
    Insert
    HLA-B*07:02
    Tags
    BSP41
    Expression
    Bacterial
    Mutation
    Replacement of transmembrane domain with enzymati…
    Available Since
    March 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    180446-plasmid-map-sequence-id-355789

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  6. Lenti-EF1alpha-dCas9-KRAB_Hygro

    Plasmid
    #192663
    Purpose
    3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance marker
    Depositor
    Jeffrey Miner
    Insert
    dCas9-KRAB
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, D839A, H840A and N863A in Cas9
    Promoter
    EF1a
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192663-plasmid-map-sequence-id-379915

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  9. AbVec2.0-mIghg3

    Plasmid
    #127160
    Purpose
    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG3 isotype
    Depositor
    Hedda Wardemann
    Insert
    Ighg3 (Ighg3 Mouse)
    Expression
    Mammalian
    Promoter
    hCMV IE1 gene promoter
    Available Since
    Nov. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    127160-plasmid-map-sequence-id-256904

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  10. Banshee Runx1FL mCherry

    Plasmid
    #80157
    Purpose
    retroviral expression of Runx1Fl and mCherry
    Depositor
    Ellen Rothenberg
    Insert
    Runx1 (Runx1 Mouse)
    Use
    Retroviral
    Tags
    HA and IRES-mCherry
    Expression
    Mammalian
    Available Since
    July 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    80157-plasmid-map-sequence-id-153318

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  11. CROPSeq-PP7-p65-HSF

    Plasmid
    #196719
    Purpose
    CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).
    Depositor
    Bernhard Schmierer
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Available Since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196719-plasmid-map-sequence-id-388066

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  13. pBac[3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40]

    Plasmid
    #86453
    Purpose
    UAS responder construct for misexpression in Tribolium; UAS drives tGFP expression; (Transformation plasmid; Red eye marker)
    Depositor
    Gregor Bucher
    Insert
    3xP3-DsRed; UAS-Tc'hsp_p-tGFP-SV40
    Expression
    Insect
    Available Since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    86453-plasmid-map-sequence-id-171157

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  14. mCherry-SiT-N-15

    Plasmid
    #55133
    Purpose
    Localization: Golgi TGN, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    SiT (ST6GAL1 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    aa 1-45 of SiT
    Promoter
    CMV
    Available Since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  15. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid
    #174893
    Purpose
    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette
    Depositor
    Simon Elsaesser
    Insert
    mCherry and EGFP
    Expression
    Mammalian
    Promoter
    EF1
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174893-plasmid-map-sequence-id-344146

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  16. pTRE3G-VP64-dCas9-VP64-ZsG

    Plasmid
    #192668
    Purpose
    Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    VP64-dCas9-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    TRE3G
    Available Since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192668-plasmid-map-sequence-id-379892

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  17. nuc-mCherry-pTRIP

    Plasmid
    #163520
    Purpose
    Used to construct lentivirus to express nucleus-targeted mCherry in mammalian cells
    Depositor
    Thomas Weber
    Insert
    nucleus targeted mCherry
    Use
    Lentiviral
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163520-plasmid-map-sequence-id-321569

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  18. pCMV-tomm20-ECFP-RspANFAST

    Plasmid
    #216348
    Purpose
    targets ECFP-RspANFAST to mitochondrial membrane
    Depositor
    Alison Tebo
    Insert
    tomm20-ECFP-RspANFAST
    Tags
    tomm20
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216348-plasmid-map-sequence-id-427395

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  19. TMC1-TwinStrepII

    Plasmid
    #83267
    Purpose
    Expression of tagged protein in mammalian cells
    Depositor
    Peter Barr-Gillespie
    Insert
    TMC1 (Tmc1 Mouse)
    Tags
    Twin Strep Tag II
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    83267-plasmid-map-sequence-id-162234

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  20. Syn-FLEX-MCS-WPRE-SV40 - GCH8

    Plasmid
    #230818
    Purpose
    AAV cloning vector for CRE-dependent expression under the Syn promoter.
    Depositor
    Kimberly Ritola
    Type
    Empty backbone
    Use
    AAV and Cre/Lox; Cloning vector
    Available Since
    Jan. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    230818-plasmid-map-sequence-id-457133

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  21. 4X3G

    Plasmid
    #64663
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SIAH1 (SIAH1 Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    April 27, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. mEmerald-Tensin1-C-14

    Plasmid
    #54274
    Purpose
    Localization: Focal Adhesions, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Tensin1 (TNS1 Human)
    Tags
    mEmerald
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  24. pCMV6-XL4 FLAG-SFRP2-Fc

    Plasmid
    #115772
    Purpose
    Expression of the extracellular domain of SFRP2 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    SFRP2 (SFRP2 Human)
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115772-plasmid-map-sequence-id-223829

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  25. pFastBacMam2_N_His8_eGFP_TEV

    Plasmid
    #178806
    Purpose
    Baculovirus gene transfer into Mammalian cells to product recombinant proteins
    Depositor
    Kazuhiro Abe
    Type
    Empty backbone
    Tags
    His8, eGFP, TEV
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Jan. 12, 2022
    Availability
    Academic Institutions and Nonprofits only
    178806-plasmid-map-sequence-id-351928

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  29. R777-E137 Hs.MTOR

    Plasmid
    #70421
    Purpose
    Gateway ORF clone of human MTOR [NM_004958.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    MTOR (MTOR Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70421-plasmid-map-sequence-id-125870

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  30. pExp-MsyB

    Plasmid
    #112570
    Purpose
    Used for expression of protein in E. coli as TEV cleavable N-terminual 8His and MsyB (acidic protein MsyB from E. coli) fusions with optional C-terminal StrepII-tag
    Depositor
    Marko Hyvönen
    Type
    Empty backbone
    Tags
    8His and Strep tag
    Expression
    Bacterial
    Available Since
    Feb. 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    112570-plasmid-map-sequence-id-255699

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  31. pJ251-GERC

    Plasmid
    #47441
    Purpose
    Expresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.
    Depositor
    George Church
    Insert
    Gene Expression Reporter Construct
    Expression
    Bacterial
    Available Since
    Aug. 27, 2013
    Availability
    Academic Institutions and Nonprofits only
    47441-plasmid-map-sequence-id-67511

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  32. pGEX-2T-RhoA-wt

    Plasmid
    #12959
    Depositor
    Gary Bokoch
    Insert
    RhoA (RHOA Human)
    Tags
    GST
    Expression
    Bacterial
    Available Since
    March 2, 2007
    Availability
    Academic Institutions and Nonprofits only

  33. pCDH-CB-FLPe-P2A-tdTomato

    Plasmid
    #72259
    Purpose
    Express FLPe and tdTomato under CB promoter
    Depositor
    Kazuhiro Oka
    Insert
    FLPe-P2A-tdTomato
    Use
    Lentiviral
    Expression
    Mammalian
    Mutation
    T230I in tdTomato (please see depositor's co…
    Promoter
    CB promoter and chicken beta-actin + CMV enhancer
    Available Since
    Jan. 25, 2016
    Availability
    Academic Institutions and Nonprofits only
    72259-plasmid-map-sequence-id-131837

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  34. pCMVHA-E2F5

    Plasmid
    #24213
    Purpose
    Mammalian expression of human E2F5 with HA tag
    Depositor
    Kristian Helin
    Insert
    E2F5 (E2F5 Human)
    Tags
    HA
    Expression
    Mammalian
    Available Since
    Dec. 15, 2010
    Availability
    Academic Institutions and Nonprofits only
    24213-plasmid-map-sequence-id-245679

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  35. pJK647

    Plasmid
    #71701
    Purpose
    Produces E. coli thioredoxin reductase (EcTrxB)
    Depositor
    T. J. Kappock
    Insert
    thioredoxin reductase
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Jan. 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71701-plasmid-map-sequence-id-131729

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  38. tet-pLKO.puro_shLuc

    Plasmid
    #110422
    Purpose
    shLuc control, silence Luc gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    Luciferase
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110422-plasmid-map-sequence-id-213315

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  39. pB6-Hipp11-DTA-Capecchi-for

    Plasmid
    #126649
    Purpose
    B6J Hipp11 targeting vector homology arms, Capecchi configuration, DTA, I-CeuI, forward cloning sites, low copy number
    Depositor
    Mario Capecchi
    Type
    Empty backbone
    Use
    Mouse Targeting
    Available Since
    June 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    126649-plasmid-map-sequence-id-247127

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  40. pcDNA3-Flag-Siah2 Ring Mutant

    Plasmid
    #122042
    Purpose
    express Siah2 RM
    Depositor
    Ze'ev Ronai
    Insert
    Siah2 RM (Siah2 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Ring mutant deletion H98, L99A, V100G, C101A
    Available Since
    June 10, 2019
    Availability
    Academic Institutions and Nonprofits only
    122042-plasmid-map-sequence-id-242454

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  42. PCDH-BCAT2

    Plasmid
    #196204
    Purpose
    overexpress BCAT2
    Depositor
    Jianjun Chen
    Insert
    BCAT2 (BCAT2 Human)
    Use
    Lentiviral
    Mutation
    NO
    Available Since
    Feb. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196204-plasmid-map-sequence-id-383812

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  43. pCAG-N2cL

    Plasmid
    #100812
    Purpose
    express rabies virus CVS N2c strain polymerase gene (N2cL)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain polymerase gene
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    100812-plasmid-map-sequence-id-189505

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  44. pCAG-N2cP

    Plasmid
    #100808
    Purpose
    express rabies virus CVS N2c strain phosphoprotein gene (N2cP)
    Depositor
    Ian Wickersham
    Insert
    rabies virus CVS N2c strain phosphoprotein gene
    Expression
    Mammalian
    Promoter
    CAG
    Available Since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    100808-plasmid-map-sequence-id-189510

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  45. R777-E173 Hs.PIK3R2

    Plasmid
    #70457
    Purpose
    Gateway ORF clone of human PIK3R2 [NM_005027.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    PIK3R2 (PIK3R2 Human)
    Use
    Gateway entry clone
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70457-plasmid-map-sequence-id-125906

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  50. IDG_SCN2B_OE_1

    Plasmid
    #161624
    Purpose
    Overexpresses SCN2B
    Depositor
    Mike McManus
    Insert
    SCN2B (SCN2B Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    161624-plasmid-map-sequence-id-334519

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Showing: 9751 - 9800 of 167210 results