We narrowed to 14,039 results for: TIM

1. pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

   Plasmid

   #138984

   Purpose

   IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

   Depositor

   Jeff Hammerbacher

   Article

   Gottschalk et al bioRxiv 2021.11.30.470597

   Insert

   TCRB (Tcrb Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAdSh.PGK.Cas9

   Plasmid

   #58253

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoter

   Depositor

   Manuel Goncalves

   Article

   Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

   Insert

   Human codon-optimized S. pyogenes Cas9
   

   Use

   Adenoviral and CRISPR

   Expression

   Mammalian

   Available Since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-ABEmax-NRCH

   Plasmid

   #136923

   Purpose

   Expresses ABEmax-NRCH in mammalian cells

   Depositor

   David Liu

   Article

   Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

   Insert

   ABEmax-NRCH
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   see manuscript

   Promoter

   CMV

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV sMTase (eGFP)

   Plasmid

   #89930

   Purpose

   Expresses sMTase system for targeted DNA methylation in mammalian cells. Contains an eGFP marker expressed off separate promoter.

   Depositor

   Carl Novina

   Article

   Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

   Insert

   dCas9-MC-IRES-MN
   

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   deactivated Cas9

   Promoter

   CMV

   Available Since

   Sept. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-p54

   Plasmid

   #35379

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

   Insert

   p54 (NONO Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   A464V

   Promoter

   CMV

   Available Since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28:LanIII

   Plasmid

   #225075

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIII)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Dec. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28:LanIII-43

   Plasmid

   #225077

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanIII-43)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28:LanII

   Plasmid

   #225066

   Purpose

   Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

   Insert

   LanA (LanII)
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDH-TfR-mApple

   Plasmid

   #179384

   Purpose

   Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

   Depositor

   Angus Johnston

   Article

   Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

   Insert

   Transferrin receptor 1 - mApple (TFRC Synthetic, Human)
   

   Use

   Lentiviral

   Tags

   mApple

   Expression

   Mammalian

   Promoter

   EF1

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCDNA5/FRT/TO_CEP41-HaloTag7_T2A_EGFP

    Plasmid

    #169327

    Purpose

    Expression of CEP41-HaloTag7 in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    CEP41-HaloTag7 (CEP41 Human)
    

    Tags

    HaloTag7

    Expression

    Mammalian

    Available Since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3 p53 S15A

    Plasmid

    #69004

    Purpose

    mammalian expression of human p53 S15A mutant.

    Depositor

    David Meek

    Article

    Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

    Insert

    p53 (TP53 Human)
    

    Expression

    Mammalian

    Mutation

    S15A

    Promoter

    CMV

    Available Since

    Oct. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti CAG-FLAG-dCas9-VPR

    Plasmid

    #114197

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA 3.1 AnxA6

    Plasmid

    #20407

    Depositor

    Carlos Enrich

    Article

    Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

    Insert

    AnxA6 (Anxa6 Rat)
    

    Expression

    Mammalian

    Available Since

    May 1, 2009

    Availability

    Academic Institutions and Nonprofits only

14. Intron-Tagging-pX330-Cas9-Blast

    Plasmid

    #159741

    Purpose

    Expresses Cas9-2A-Blast and a sgRNA excising EGFP flanked by a splice acceptor and a splice donor from the Intron-Tagging-EGFP-Donor plasmid.

    Depositor

    Stefan Kubicek

    Article

    Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

    Insert

    donor plasmid targeting sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAluYa5-neo-TET

    Plasmid

    #51283

    Purpose

    expresses Alu, a human SINE, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassette

    Depositor

    Astrid Roy-Engel

    Article

    Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

    Insert

    AluYa5
    

    Tags

    neoTET cassette with a tetrahymena self-splicing …

    Expression

    Mammalian

    Promoter

    upstream pol III enhancer sequence of the 7SL gen…

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pet28a-His6-Halo-TEV-Nrf2

    Plasmid

    #62455

    Purpose

    Halo-His6 tagged Nrf2 for E.coli expression

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Nrf2 (NFE2L2 Human)
    

    Tags

    His6-Halo-TEV

    Expression

    Bacterial

    Available Since

    April 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEF1a-hMLH1NTD-NLS

    Plasmid

    #174826

    Purpose

    Mammalian expression of human MLH1NTD-NLS (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    human MLH1_NTD-NLS (codon optimized)
    

    Tags

    SV40 bpNLS

    Expression

    Mammalian

    Mutation

    truncation of residues 1-335

    Promoter

    EF1a

    Available Since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

    Plasmid

    #62458

    Purpose

    expresses his-tagged C151S human Keap1 protein

    Depositor

    Yimon Aye

    Article

    Parvez et al J Am Chem Soc. 2014 Dec 31.

    Insert

    Keap1 (KEAP1 Human)
    

    Tags

    His-Halo-Tev

    Expression

    Mammalian

    Mutation

    Changed Cysteine 151 of Keap1 to serine

    Promoter

    CMV

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. h-Plekhm1_EGFP

    Plasmid

    #73836

    Purpose

    Express human Plekhm1 in mammalian cells

    Depositor

    Paul Odgren

    Article

    Van Wesenbeeck et al J Clin Invest. 2007 Apr;117(4):919-30.

    Insert

    Plekhm1 (PLEKHM1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. Dominant negative Hsp90 beta

    Plasmid

    #22480

    Depositor

    William Sessa

    Article

    Miao et al Arterioscler Thromb Vasc Biol. 2008 Jan

    Insert

    Dominant negative Hsp90 (HSP90AB1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Dec. 8, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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