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pcDNA3.1_pCMV-nCas-PmCDA1-ugi pH1-gRNA(HPRT)

Plasmid

#79620

Purpose

Expresses nCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells

Depositor

Akihiko Kondo

Publication

Nishida et al Science. 2016 Aug 4. pii: aaf8729.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SpCas9

Tags

PmCDA1 and UGI

Expression

Mammalian

Mutation

D10A for nickase Cas9

Promoter

pCMV

Available since

July 27, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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pMSCV-Zeo

Plasmid

#75088

Purpose

retrovirus-mediated gene transfer into mammalian cells

Depositor

David Mu

Publication

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pYPQ152

Plasmid

#69303

Purpose

Gateway entry vector with pco-dCas9-VP64

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pco-dCas9-VP64 (Plant codon-optimized)

Use

CRISPR

Mutation

VP64 activator fusion; NLS fusion

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDD284

Plasmid

#66825

Purpose

TagRFP-T^SEC^3xFlag vector with ccdB markers for cloning homology arms

Depositor

Bob Goldstein

Publication

Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

Insert

TagRFP-T-C1^SEC^3xFlag

Use

CRISPR and Cre/Lox

Tags

3xFlag and C. elegans codon-optimized TagRFP-T

Expression

Worm

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles
pRRL-MHCK7-GCaMP6

Plasmid

#65042

Purpose

Lentiviral vector expressing the calcium indicator GCaMP6 driven by the muscle-specific promoter MHCK7

Depositor

Nenad Bursac

Publication

Madden et al eLife. 2015 Jan 9;4:e04885. doi: 10.7554

Insert

GCaMP6

Use

Lentiviral

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

MHCK7

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pSIN4-EF1a-ETV2-IRES-Puro

Plasmid

#61061

Purpose

lentiviral vector for ETV2

Depositor

Igor Slukvin

Publication

Elcheva et al Nat Commun. 2014 Jul 14;5:4372. doi: 10.

Insert

ETV2 (ETV2 Human)

Use

Lentiviral

Promoter

EF1alpha

Available since

March 27, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pSBbi-Neo

Plasmid

#60525

Purpose

SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: neomycin resistance gene

Depositor

Eric Kowarz

Publication

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

EF1a/RPBSA

Available since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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phDLX2-N174

Plasmid

#60860

Purpose

2nd generation lentiviral transfer plasmid. Expresses DLX2 under the EF1a promoter

Depositor

Andrew Yoo

Publication

Victor et al Neuron. 2014 Oct 22;84(2):311-23. doi: 1

Insert

DLX2 (DLX2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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mEmerald-CD81-10

Plasmid

#54031

Purpose

Localization: Tetraspanin/Membrane, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Publication

He et al PLoS Pathog. 2013;9(10):e1003701. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

CD81 (CD81 Human)

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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pICH47742::2x35S-5’UTR-hCas9(STOP)-NOST

Plasmid

#49771

Purpose

Level 1 hCas9 module

Depositor

Sophien Kamoun

Publication

Belhaj et al Plant Methods. 2013 Oct 11;9(1):39.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

35Sp::hCas9

Use

CRISPR

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

26 Articles

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paavCAG-Jx-rev-post-mGRASP-2A-dTomato

Plasmid

#34913

Purpose

JxON-post

Depositor

Jinhyun Kim

Publication

Kim et al Nat Methods. 2011 Dec 4;9(1):96-102. doi

Insert

postsynaptic mGRASP

Use

AAV and Cre/Lox

Promoter

CAG

Available since

Nov. 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pMito-iRFP670

Plasmid

#45462

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

iRFP670

Tags

human cytochrome C oxidase subunit VIII

Expression

Mammalian

Mutation

T2A, S45T, V116I, D124E, M170L, K180M, D202L, I20…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEGFP-TRIM28

Plasmid

#45568

Depositor

Fanxiu Zhu

Publication

Liang et al J Immunol. 2011 Nov 1;187(9):4754-63. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRIM28 (TRIM28 Human)

Tags

EGFP

Expression

Mammalian

Mutation

G34S mutation in EGFP

Promoter

CMV

Available since

May 29, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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human EB1 fused to monomeric RFP

Plasmid

#39323

Depositor

Tim Mitchison, Jennifer Tirnauer

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

EB1 (MAPRE1 Human)
mRFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pBB550

Plasmid

#27396

Depositor

Bernd Bukau

Publication

de Marco et al BMC Biotechnol. 2007 Jun 12;7:32. doi: 1

Insert

dnaK, dnaJ, GroESL

Expression

Bacterial

Available since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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beta-arrestin1 mYFP

Plasmid

#36916

Depositor

Robert Lefkowitz

Publication

Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

beta-arrestin1 (Arrb1 Rat)

Tags

mYFP

Expression

Mammalian

Promoter

CMV

Available since

June 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pET28a-SpyTagMBP

Plasmid

#35050

Depositor

Mark Howarth

Publication

Zakeri et al Proc Natl Acad Sci U S A. 2012 Mar 20;10

Insert

SpyTagMBP

Tags

His6

Expression

Bacterial

Mutation

Simple peptide tag added to the N-terminus of MBP…

Promoter

T7 promoter

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLenti-PGK-ER-KRAS(G12V)

Plasmid

#35635

Depositor

Daniel Haber

Publication

Singh et al Cell. 2012 Feb 17;148(4):639-50.

Insert

K-Ras4B(G12V) (KRAS Human)

Use

Lentiviral

Tags

ER

Expression

Mammalian

Mutation

Glycine 12 to Valine

Promoter

PGK

Available since

April 18, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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B23(NPM)

Plasmid

#34553

Depositor

Mary Dasso

Publication

Yun et al J Cell Biol. 2008 Nov 17;183(4):589-95.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

B23/nucleophosmin (NPM1 Human)

Tags

DsRed

Expression

Mammalian

Promoter

CMV

Available since

Dec. 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pcDNA-SADB19P

Plasmid

#32631

Depositor

Edward Callaway

Publication

Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SADB19P

Expression

Mammalian

Promoter

CMV

Available since

Oct. 26, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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