We narrowed to 13,642 results for: NTS

1. pDECKO_UCA1

   Plasmid

   #72628

   Purpose

   Expresses two gRNAs targeting the UCA1 promoter

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Aparicio-Prat et al BMC Genomics. 2015 Oct 23;16(1):846. doi

   Insert

   gRNAs toward UCA1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (gRNA1) and H1 (gRNA2)

   Available since

   June 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDGE497

   Plasmid

   #153249

   Purpose

   M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX4T2-Snf1-cat

   Plasmid

   #52683

   Purpose

   expression of the Snf1 kinase domain in bacteria

   Depositor

   Benjamin Turk

   Article

   Lee et al Mol Cell Biol. 2012 Nov;32(22):4705-17.

   Insert

   SNF1 (SNF1 Budding Yeast)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   encodes kinase domain only, aa 41-315

   Promoter

   tac

   Available since

   April 14, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pAAVS1 Insulator ObLiGaRe/EPB71

   Plasmid

   #90018

   Purpose

   Donor vector for ObLiGaRe/ZFN mediated targeting to AAVS1 locus

   Depositor

   Eric-Paul Bennett

   Article

   Pinto et al Nucleic Acids Res. 2017 May 4. doi: 10.1

   Insert

   MCS flanked by insulator and inverted ZFN binding sites
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Cdc42 T17N sp64T

   Plasmid

   #16672

   Depositor

   De-Li Shi

   Article

   Djiane et al Development. 2000 Jul . 127(14):3091-100

   Insert

   Cdc42 T17N (CDC42 Human)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   T17N. Dominant negative.

   Promoter

   Available since

   April 28, 2008

   Availability

   Academic Institutions and Nonprofits only

6. prNFL-L269P-EGFP

   Plasmid

   #132601

   Purpose

   Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E L269P mutation

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   Nefl (Nefl Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   806T>C, 807G>C; Leu269Pro

   Promoter

   CMV

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. prNFL-P441L-EGFP

   Plasmid

   #132604

   Purpose

   Expresses EGFP-tagged rat NFL containing the Charcot-Marie-Tooth disease type 2E P441L mutation

   Depositor

   Anthony Brown

   Article

   Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

   Insert

   Nefl (Nefl Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   1322C>T, 1323A>G; Pro441Leu

   Promoter

   CMV

   Available since

   Nov. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-SLC30A10-N43H

   Plasmid

   #82344

   Purpose

   Expresses FLAG-tagged SLC30A10-N43H in mammalian cells

   Depositor

   Somshuvra Mukhopadhyay

   Article

   Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

   Insert

   SLC30A10 (SLC30A10 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N43H

   Promoter

   CMV

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pTH353-SUP45wt

   Plasmid

   #29371

   Depositor

   Tobias von der Haar

   Article

   Merritt et al Nucleic Acids Res. 2010 Sep . 38(16):547

   Insert

   SUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Wild type gene including genomic sequence 1432 bp…

   Promoter

   Available since

   May 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FLAG-SLC30A10-D248A

    Plasmid

    #82346

    Purpose

    Expresses FLAG-tagged SLC30A10-D248A in mammalian cells

    Depositor

    Somshuvra Mukhopadhyay

    Article

    Zogzas et al J Biol Chem. 2016 Jul 29;291(31):15940-5

    Insert

    SLC30A10 (SLC30A10 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D248A

    Promoter

    CMV

    Available since

    Sept. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. KHBD00409

    Plasmid

    #34517

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG5249 (Blimp-1 Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pAtCas9-dead_2

    Plasmid

    #91166

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_D10A+H840A (dead), Plant Selection: 2x35S:npt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_D10A+H840A (dead)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A, H840A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. FusionRed-PMP-N-10

    Plasmid

    #56131

    Purpose

    Localization: Peroxisomes, Excitation: 580, Emission: 608

    Depositor

    Michael Davidson

    Article

    Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

    Insert

    PMP (PXMP2 Human)
    

    Use

    Tags

    FusionRed

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

14. pTaCas9-H840A_6

    Plasmid

    #91173

    Purpose

    Cas9 only expression, Cas9 Type: TaCas9_H840A (nickase), Plant Selection: PvUbi2:bar

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    TaCas9_H840A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A

    Promoter

    Available since

    July 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. MDH1-PGK-FLAG-TAT-86-A

    Plasmid

    #35977

    Depositor

    Susana Valente

    Article

    Mediouni et al Curr HIV Res. 2015;13(1):64-79.

    Insert

    transactivating protein (tat Human Immunodeficiency Virus)
    

    Use

    RNAi and Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    From the "A" HIV clade

    Promoter

    Available since

    April 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGWB422

    Plasmid

    #74816

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with GST (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGWB428

    Plasmid

    #74822

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with TAP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    July 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDGE660

    Plasmid

    #153258

    Purpose

    M4E shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. KHBD00671

    Plasmid

    #39722

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG6312 (Rfx Fly)
    

    Use

    Gateway donr vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Aug. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pYPQ133D2.0

    Plasmid

    #99894

    Purpose

    Golden Gate entry vector to express the 3rd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYPQ131D2.0

    Plasmid

    #100044

    Purpose

    Golden Gate entry vector to express the 1st gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under OsU3 promoter

    Depositor

    Yiping Qi

    Article

    Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    OsU3

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pAtCas9-H840A_1

    Plasmid

    #91162

    Purpose

    Cas9 only expression, Cas9 Type: AtCas9_H840A (nickase), Plant Selection: 2x35S:hpt II

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Insert

    AtCas9_H840A (nickase)
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A

    Promoter

    Available since

    Sept. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pT7-AsCas12a_crRNA-site3 (RTW549)

    Plasmid

    #160138

    Purpose

    T7 promoter expression plasmid for in vitro transcription of AsCas12a crRNA with spacer #3

    Depositor

    Benjamin Kleinstiver, Keith Joung

    Article

    Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

    Insert

    AsCas12a crRNA with spacer #3 (spacer=CTGATGGTCCATGTCTGTTACTC)
    

    Use

    In vitro transcription of sgrna from t7 promoter

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pYPQ292 (AaCas12b)-D570A-TV

    Plasmid

    #136380

    Purpose

    CRISPRa Gateway entry clone for catalytically dead AaCas12b (D570A) fused with TV activation system

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b (D570A)-TV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D570A

    Promoter

    Available since

    Feb. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pENTR1A-3xFlag-S242R-MEFV

    Plasmid

    #167020

    Purpose

    Gateway entry plasmid with 3xFlag-pyrin (S242R/PAAND variant)

    Depositor

    Thomas Henry

    Article

    Chirita et al Methods Mol Biol. 2022;2523:179-195. doi

    Insert

    MEFV (MEFV Human)
    

    Use

    Gateway entry vector

    Tags

    3xFlag

    Expression

    Mutation

    serine 242 in arginine (S242R)

    Promoter

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA5/FRT/TO - RBM28 E5 WT minigene

    Plasmid

    #169269

    Purpose

    Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

    Depositor

    Susan Baserga

    Article

    Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

    Insert

    RBM28 E5 WT minigene (RBM28 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pJAG98-mTurquoise2-FHA2-AURKB substrate-YPet

    Plasmid

    #83286

    Purpose

    untargeted Aurora B FRET sensor with mTurquoise2 and YPet FRET pair

    Depositor

    Daniel Needleman

    Article

    Yoo et al Elife. 2018 Jul 25;7. pii: 36392. doi: 1

    Insert

    mTurquoise2-FHA2-AURKB substrate-YPet
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. KHBD00340

    Plasmid

    #34463

    Depositor

    Bart Deplancke

    Article

    Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

    Insert

    CG8573 (su(Hw) Fly)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

29. pAT9993-Hypa-ABE

    Plasmid

    #163001

    Purpose

    plasmid expressing Hypa-ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    Hypa-ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAT9676-ABE

    Plasmid

    #162997

    Purpose

    plasmid expressing ABE

    Depositor

    Ervin Welker

    Article

    Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

    Insert

    ABE
    

    Use

    CRISPR

    Tags

    3xFLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a

    Available since

    Sept. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge