We narrowed to 11,205 results for: SHA

1. pGFP-Ub-VPS13D_dC

   Plasmid

   #176466

   Purpose

   express VPS13D lacking C-terminal 407 aa in mammalian cells

   Depositor

   Richard Youle

   Article

   Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

   Insert

   VPS13D (VPS13D Human)
   

   Use

   Tags

   GFP-Ub

   Expression

   Mammalian

   Mutation

   C-terminal 407 aa is deleted.

   Promoter

   CMV

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. kank2_pJet1.2

   Plasmid

   #121980

   Purpose

   for generating kank2 riboprobes for WISH in zebrafish

   Depositor

   GuangJun Zhang

   Article

   Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

   Insert

   kank2 (LOC571549 Zebrafish)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-myc-mCE(K294A)

   Plasmid

   #82461

   Purpose

   Expression of myc epitope and catalytic inactive form of murine mRNA Capping Enzyme (K294A) in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

   Insert

   mCE (K294A) (Rngtt Mouse)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Lysine 294 changed to Alanine

   Promoter

   CMV

   Available since

   Sept. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUAST-HDAC4 3A

   Plasmid

   #52978

   Purpose

   insect expression of HDAC4 3A constitutive active mutant

   Depositor

   Marc Montminy, Biao Wang

   Article

   Wang et al Cell. 2011 May 13;145(4):596-606. doi: 1

   Insert

   HDAC4 (HDAC4 Fly)
   

   Use

   Tags

   FLAG

   Expression

   Insect

   Mutation

   S239,573,748A constitutively active*

   Promoter

   Hsp70

   Available since

   July 29, 2014

   Availability

   Academic Institutions and Nonprofits only

5. Clover-ZO1-C-14

   Plasmid

   #56375

   Purpose

   Localization: Tight Junctions, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   ZO1 (TJP1 Human)
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

6. PDZK1_PDZ_1

   Plasmid

   #103927

   Purpose

   Protein expression and purification of PDZK1 PDZ1 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDZK1 (PDZK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SLC9A3R2_PDZ_2

   Plasmid

   #103926

   Purpose

   Protein expression and purification of NHERF2 PDZ2 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   SLC9A3R2 (NHERF2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. PDZK1_PDZ_4

   Plasmid

   #103945

   Purpose

   Protein expression and purification of PDZK1 PDZ4 domain

   Depositor

   Sachdev Sidhu

   Article

   Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

   Insert

   PDZK1 (PDZK1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLN481 (FuGW-G8p-IL12-1Pe)

   Plasmid

   #105191

   Purpose

   Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of IL12 transcripts containing miR1-Bs(Pe)

   Depositor

   Timothy Lu

   Article

   Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

   Insert

   G8p-IL12-1Pe
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKS-DTA miR-106b~25

    Plasmid

    #19030

    Depositor

    Tyler Jacks

    Article

    Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

    Insert

    microRNA 106a-25 (Mir25 Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. SMT205

    Plasmid

    #134817

    Purpose

    SMT201 with mutated RBS for DHFR, in vivo assays

    Depositor

    Tanja Kortemme

    Article

    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1

    Inserts

    Dihydrofolate reductase
    Thymidylate synthase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Silent mutations to eliminate internal restrictio…

    Promoter

    T7 and TET

    Available since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDB368

    Plasmid

    #110276

    Purpose

    gHH_44 with a N-terminal 10-His DsbC TEV-peptide fusion

    Depositor

    Christopher Bahl

    Article

    Buchko et al Protein Sci. 2018 Aug 26. doi: 10.1002/p

    Insert

    gHH_44
    

    Use

    Tags

    10-His, Tev recognition peptide, and mature DsbC …

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEJS430-pCSDest2-AcrIIC1Boe

    Plasmid

    #85678

    Purpose

    Mammalian expression of Type II-C anti-CRISPR protein AcrIIC1Boe from Brackiella oedipodis

    Depositor

    Erik Sontheimer

    Article

    Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

    Insert

    Type II-C anti-CRISPR AcrIIC1Boe
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTPTP alpha (S204A)

    Plasmid

    #17694

    Depositor

    Xinmin Zheng

    Article

    Zheng et al J Biol Chem. 2002 Jun 14. 277(24):21922-

    Insert

    PTPalpha S204A (PTPRA Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S204A

    Promoter

    Available since

    April 24, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA-h-mmPtch1-B-HA

    Plasmid

    #120902

    Purpose

    Expresses Ptch1-B for function assays

    Depositor

    Philip Beachy

    Article

    Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

    Insert

    ptch1 (Ptch1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deleted amino acids 620-643, truncated at 1291

    Promoter

    CMV

    Available since

    Jan. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. TJP2_PDZ_3

    Plasmid

    #103949

    Purpose

    Protein expression and purification of X104 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP2 (TJP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TJP2_PDZ_2

    Plasmid

    #103955

    Purpose

    Protein expression and purification of X104 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    TJP2 (TJP2 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-EF1a-FLEX-T-RG

    Plasmid

    #102368

    Purpose

    Express cre dependent TVA and SADB19 RG (biscistronic) as an AAV

    Depositor

    Troy Margrie

    Article

    Velez-Fort et al Neuron. 2018 Apr 4;98(1):179-191.e6. doi

    Insert

    TVA E2A-RG
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pKP4010

    Plasmid

    #113336

    Purpose

    Beta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoter

    Depositor

    Suzanne Sandmeyer

    Article

    Patterson et al Genome Res. 2019 Aug;29(8):1298-1309. do

    Inserts

    Artificial Transcription Factor (McIsaac et al, 2013)
    Ty3 Integrase
    

    Use

    Tags

    3X-FLAG/8X-Gly linker

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HM3C NaVSp1_p pET24b

    Plasmid

    #127852

    Purpose

    Bacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.

    Depositor

    Dan Minor

    Article

    Arrigoni et al Cell. 2016 Feb 25;164(5):922-36. doi: 10

    Insert

    NaVSp1
    

    Use

    Tags

    3C Cleavage site, His Tag, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pBS-miR106a-363

    Plasmid

    #19028

    Depositor

    Tyler Jacks

    Article

    Ventura et al Cell. 2008 Mar 7. 132(5):875-86.

    Insert

    microRNA 106a-363 (Mir106a Mouse)
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 25, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. A3H HapI-Cas9n-UGI

    Plasmid

    #119140

    Purpose

    Base editor made from APOBEC3H Haplotype I

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3H Haplotype I-Cas9 nickase-UGI
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    None

    Promoter

    CMV

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pJL1-aadA

    Plasmid

    #117050

    Purpose

    In vitro expression of aadA (streptomycin resistance gene) from the T7 promoter

    Depositor

    Michael Jewett

    Article

    Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

    Insert

    aadA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pFA6a-kanMX6-P3nmt1-GST

    Plasmid

    #39286

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    nmt1 promoter (nmt1 Fission Yeast)
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    -1163 to +6 of nmt1 locus

    Promoter

    Available since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRSM32

    Plasmid

    #101634

    Purpose

    Encodes the fluorescent protein Rosmarinus for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    Rosmarinus
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Clover-SiT-N-15

    Plasmid

    #56306

    Purpose

    Localization: Golgi TGN, Excitation: 505, Emission: 515

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    SiT (ST6GAL1 Human)
    

    Use

    Tags

    Clover

    Expression

    Mammalian

    Mutation

    aa 1-45 of SiT

    Promoter

    CMV

    Available since

    Dec. 5, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pThom77.2

    Plasmid

    #101646

    Purpose

    Encodes the fluorescent protein amFP486 for constitutive expression in E. coli.

    Depositor

    Thomas Hughes

    Article

    Molina et al J Phys Chem Lett. 2017 Jun 15;8(12):2548

    Insert

    amFP486
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. MPDZ_PDZ_7

    Plasmid

    #103891

    Purpose

    Protein expression and purification of Multiple PDZ protein PDZ7 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MPDZ (MPDZ Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. MPDZ_PDZ_13

    Plasmid

    #103897

    Purpose

    Protein expression and purification of Multiple PDZ protein PDZ13 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MPDZ (MPDZ Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. MPDZ_PDZ_3

    Plasmid

    #103887

    Purpose

    Protein expression and purification of Multiple PDZ protein PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MPDZ (MPDZ Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge