We narrowed to 19,527 results for: ace

1. plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

   Plasmid

   #209724

   Purpose

   Expresses cytosolic low affinity ratiometric ATP sensor

   Depositor

   Timothy Ryan

   Article

   Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

   Insert

   (cyto).iATPSnFR2.A95A.A119L.HaloTag
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CamKII

   Available since

   Nov. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. HiFi Cas9

   Plasmid

   #188490

   Purpose

   Expresses FLAG tagged HiFi Cas9

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   R691A

   Promoter

   EF1a

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pKDsgRNA-rssA

   Plasmid

   #89957

   Purpose

   Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

   Depositor

   Michael Lynch

   Article

   Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

   Insert

   rssA gRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pTet

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-FLEX-EGFP-KASH

   Plasmid

   #154373

   Purpose

   Vector for Cre-dependent expression of EGFP tagged KASH protein domain

   Depositor

   Larry Zweifel

   Article

   Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

   Insert

   EGFP-KASH
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTSAR3.5t

   Plasmid

   #210257

   Purpose

   Fluorescent reporter for Type III secretion system activity in Shigella flexneri

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Inserts

   eBFP2
   superfolder GFP
   

   Use

   Tags

   ssra

   Expression

   Bacterial

   Mutation

   Promoter

   ipaH7.8p and rpsM

   Available since

   Feb. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-DIO-Cas9

   Plasmid

   #166601

   Purpose

   Encodes Cre-dependent Cas9 under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   Cas9
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   tetracycline response element

   Available since

   April 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTSAR3.1t

   Plasmid

   #210254

   Purpose

   Fluorescent reporter for Type III secretion system activity in Shigella flexneri

   Depositor

   François-Xavier Campbell-Valois

   Article

   Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

   Inserts

   mCerulean
   superfolder GFP
   

   Use

   Tags

   ssra

   Expression

   Bacterial

   Mutation

   Promoter

   ipaH7.8p and rpsM

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCS2_Firefly_degron-60A

   Plasmid

   #200396

   Purpose

   A plasmid IVT template containing a destabilized Firefly luciferase-PEST (degron) fusion followed by a template-encoded poly(A) tail. Linearization at an Esp3i site.

   Depositor

   Xiao Wang

   Article

   Aditham et al ACS Chem Biol. 2022 Dec 16;17(12):3352-3

   Insert

   Firefly luciferase - degron
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn1-FLEX-CybGam-WPRE

   Plasmid

   #207662

   Purpose

   Control for CybSEP2

   Depositor

   Sung Han

   Article

   Kim et al bioRxiv 2023.01.19.524797

   Insert

   Gamillus
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Histidine 86 and 159 to alanines, inserted gamill…

   Promoter

   hSynapsin

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-GFP1-10-HygR

    Plasmid

    #223675

    Purpose

    To check the split GFP signals in mitochondria (1)

    Depositor

    Juan Melero-Martin

    Article

    Lin et al Nature. 2024 May;629(8012):660-668. doi:

    Insert

    GFP1-10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Nov. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAS_4xMma PylT_GFP 150TAG 6xHis

    Plasmid

    #140015

    Purpose

    Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

    Insert

    sfGFP
    

    Use

    Tags

    6xHis

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available since

    June 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-mBeRFP

    Plasmid

    #175173

    Purpose

    large-stokes shift red fluorescent protein mBeRFP

    Depositor

    Bernardo Sabatini

    Article

    Melzer et al Cell. 2021 Oct 28;184(22):5622-5634.e25.

    Insert

    mBeRFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.CAG.(mito).cpSFGFP.HaloTag

    Plasmid

    #214925

    Purpose

    Expresses mitochondrially targeted non-responsive control

    Depositor

    Timothy Ryan

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    (mito).cpSFGFP.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLPO2

    Plasmid

    #209976

    Purpose

    Contains Level 0 Part: replication origin (SH71rep) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (SH71_rep)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCD-TEVP

    Plasmid

    #226225

    Purpose

    Express TEVP

    Depositor

    Michael Elowitz

    Article

    Xia et al Cell. 2024 Apr 16:S0092-8674(24)00347-7.

    Insert

    TEVP (TEVgp1 Tobacco etch virus)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFP-N1-Cdc45

    Plasmid

    #211426

    Purpose

    mammalian expression of human Cdc45 C-terminally fused to EGFP

    Depositor

    Helmut Pospiech

    Article

    Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

    Insert

    Cdc45 (CDC45 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLPO1

    Plasmid

    #209975

    Purpose

    Contains Level 0 Part: replication origin (256rep) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    replication origin (256_rep)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBAD-Lime

    Plasmid

    #191255

    Purpose

    Bacterial expression plasmids for pH sensitive fluorescent protein Lime

    Depositor

    Robert Campbell

    Article

    Shen et al ACS Sens. 2023 Aug 25;8(8):3014-3022. do

    Insert

    Lime
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-mStayGold-EFS-Blast

    Plasmid

    #227257

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mStayGold-EFS-Blast cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV.hSynapsin.(cyto).cpSFGFP.HaloTag

    Plasmid

    #209666

    Purpose

    Non-responsive control

    Depositor

    Tim Brown, HHMI-JRC Tool Translation Team

    Article

    Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

    Insert

    cpSFGFP.HaloTag
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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