We narrowed to 4,895 results for: phy

1. pCMV6-AN-HA_WT POLH

   Plasmid

   #201671

   Purpose

   Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

   Depositor

   Roger Woodgate

   Article

   McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

   Insert

   Polymerase eta (POLH Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Coding sequence has been optimised for expression…

   Promoter

   CMV

   Available since

   June 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. R701-X65-527: His6-tev-Hs.CALM1(2-148)

   Plasmid

   #159693

   Purpose

   E. coli protein expression of His6-tev-Hs.CALM1(2-148)

   Depositor

   Dominic Esposito

   Article

   Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

   Insert

   His6-tev-Hs.CALM1(2-148) (CALM1 Human)
   

   Use

   Tags

   His6-tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-15b_LC3B

   Plasmid

   #73949

   Purpose

   For recombinant expression of human LC3B/MAP1LC3B in E. coli

   Depositor

   Dieter Willbold

   Article

   Ma et al J Biol Chem. 2013 Dec 27;288(52):37204-1

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   March 25, 2016

   Availability

   Academic Institutions and Nonprofits only

4. mini-YFP-hDF-C2F-TM

   Plasmid

   #72600

   Purpose

   mini human dysferlin (from domain F to transmembrane ) tagged with YFP

   Depositor

   Renzhi Han

   Article

   Cheng et al Nat Med. 2014 Oct;20(10):1187-92. doi: 1

   Insert

   dysferlin (DYSF Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   from domain F to transmembrane domain

   Promoter

   CMV

   Available since

   April 15, 2016

   Availability

   Academic Institutions and Nonprofits only

5. GABA(A) receptor subunit a1SE

   Plasmid

   #49168

   Purpose

   pHluorin-tagged GABA A receptor subunit (alpha 1) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface

   Depositor

   Tija Jacob, Stephen Moss

   Article

   Tretter et al J Neurosci. 2008 Feb 6;28(6):1356-65. do

   Insert

   GABA(A) receptor subunit alpha-1 (Gabra1 Mouse)
   

   Use

   Tags

   myc and pHGFP (pHIuorin)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-Synapsin-pHoenix-WPRE

   Plasmid

   #70111

   Purpose

   lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

   Depositor

   Christian Rosenmund

   Article

   Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

   Insert

   pHoenix (Syp Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Rat)
   

   Use

   Lentiviral

   Tags

   Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcrDNA3.1-PRKACA

   Plasmid

   #100890

   Purpose

   Mammalian expression of PRKACA

   Depositor

   Sanford Simon

   Article

   Honeyman et al Science. 2014 Feb 28;343(6174):1010-4. d

   Insert

   Protein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Isoform 1 wild type

   Promoter

   CMV

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJet-CMV-IARS1-GFP

   Plasmid

   #212322

   Purpose

   EGFP-tagged IARS1

   Depositor

   Sabine Fuchs

   Article

   Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

   Insert

   IARS (IARS1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Wildtype

   Promoter

   CMV

   Available since

   Nov. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCI-neo:Beta2meGFP

   Plasmid

   #45096

   Depositor

   Henry Lester

   Article

   Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

   Insert

   nAChR beta2 (Chrnb2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   There are strain differences between C57BJ/6 and …

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRL_GI_PCB28

    Plasmid

    #98745

    Purpose

    For phycocyanobilin production in Saccharomyces cerevisiae. Contains integrative cassette for the yeast his1-Δ200 locus. Encodes constitutively expressed HY1 and PcyA.

    Depositor

    Bernd Müller-Röber

    Article

    Hochrein et al Nucleic Acids Res. 2017 Sep 6;45(15):919

    Inserts

    heme oxygenase 1 (TED4 Mustard Weed)
    Phycocyanobilin:ferredoxin oxidoreductase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for Saccharomyces cerevisiae

    Promoter

    ADH1m and PGK1

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. R701-X71-527: His6-tev-Hs.CALM1(1-80)

    Plasmid

    #159694

    Purpose

    E. coli protein expression of His6-tev-Hs.CALM1(1-80)

    Depositor

    Dominic Esposito

    Article

    Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

    Insert

    His6-tev-Hs.CALM1(1-80) (CALM1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R701-X72-527: His6-tev-Hs.CALM1(76-148)

    Plasmid

    #159695

    Purpose

    E. coli protein expression of His6-tev-Hs.CALM1(76-148)

    Depositor

    Dominic Esposito

    Article

    Agamasu et al Biophys J. 2019 Mar 19;116(6):1049-1063.

    Insert

    His6-tev-Hs.CALM1(76-148) (CALM1 Human)
    

    Use

    Tags

    His6-tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCC_04 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139089

    Purpose

    Expresses human codon-optimized xCas9-NG nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    A262T,R324L, S409I, E480K, E543D, M694I, L1111R, …

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNK7187

    Plasmid

    #219761

    Purpose

    MoClo-compatible Level 1 for expression PpASCL in plants (N.benthamiana, BY2 cells)

    Depositor

    Karen Sarkisyan

    Article

    Palkina et al Sci Adv. 2024 Mar 8;10(10):eadk1992. doi

    Insert

    PpASCL
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    p35s_0.4kb - 5'UTR TMV omega

    Available since

    July 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHL-H1-ccdB-mEF1a-RiH

    Plasmid

    #60601

    Purpose

    Cloning vector for CRISPR-sgRNA (into the BamHI-EcoRI site), expresses RFP and hygromycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Inserts

    Red Fluorescent Protein
    Hygromycin resistance gene
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAF92

    Plasmid

    #135929

    Purpose

    All-in-one Sleeping Beauty vector for Expression for Tasmanian devil mCherry-TNFSF9 (type II transmembrane) fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. TNFSF9).

    Depositor

    Andrew S. Flies

    Article

    Flies et al Sci Adv. 2020 Jul 1;6(27). pii: 6/27/eab

    Insert

    mCherry fused to TNFSF9
    

    Use

    Transposon

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pB80-SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)_P2A_iLID-mCherry-RAB11

    Plasmid

    #174645

    Purpose

    Optogenetic coupling of RAB11 to tetramerized moss kinesin-14 to induce retrograde transport of recycling endosomes

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    SSPB(micro)-mVenus-GCN4-ppKin14VIb(861-1321)-P2A-ILID-mCherry-RAB11
    

    Use

    Tags

    SSPB(micro)-Venus and iLID-mCherry

    Expression

    Mammalian

    Mutation

    SSPB:Arg73Gln; mVenus: Met1Del, Thr154Met; ppKin1…

    Promoter

    Chicken beta-actin

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV6-AN-DDK_WT POLI

    Plasmid

    #131228

    Purpose

    Expression of WT DNA polymerase iota with an N-terminal FLAG tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

    Insert

    DNA polymerase iota (POLI Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Coding sequence has been codon optimised for expr…

    Promoter

    CMV enhancer + CMV promoter

    Available since

    Dec. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHL-EF1a-SphcCas9-iP-A

    Plasmid

    #60599

    Purpose

    Expresses human codon-optimized Cas9 (derived from Streptococcus pyogenes) and pruomycin resistance gene.

    Depositor

    Akitsu Hotta

    Article

    Li et al Stem Cell Reports. 2014 Nov 25. pii: S22

    Insert

    CRISPR Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Codon usage optimized for human usage

    Promoter

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hsyn-flex-Voltron-ST

    Plasmid

    #119036

    Purpose

    Cre-dependant expression of soma-localized Voltron in neurons

    Depositor

    Eric Schreiter

    Article

    Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

    Has Service

    AAV1

    Insert

    Voltron-ST
    

    Use

    AAV and Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hsyn

    Available since

    Dec. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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