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pLenti-puro-ARID1A

Plasmid

#39478

Purpose

Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-DTR-GFP

Plasmid

#124364

Purpose

conditional expression of a diphtheria toxin receptor (DTR)–GFP fusion protein

Depositor

Eiman Azim, Thomas Jessell

Article

Azim et al Nature. 2014 Apr 17;508(7496):357-63. do

Has Service

AAV2

Insert

DTR-GFP

Use

AAV

Tags

GFP

Promoter

Chicken B-actin

Available Since

Oct. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCRZeroT

Plasmid

#120276

Purpose

Bifunctional vector for TA-cloning and blunt-end cloning of PCR-products using ZERO-backgound system

Depositor

Ken Motohashi

Article

Motohashi et al Sci Rep. 2019 Apr 23;9(1):6417. doi: 10.

Type

Empty backbone

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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qTAG-AAVS1-PGK-Zeo

Plasmid

#227271

Purpose

Empty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG_PSD95.FingR-eGFP-CCR5TC

Plasmid

#46295

Depositor

Don Arnold

Article

Gross et al Neuron. 2013 Jun 19;78(6):971-85. doi: 1

Insert

PSD95.FingR-eGFP-CCR5TC (DLG4 Synthetic, Human)

Expression

Mammalian

Promoter

CAG

Available Since

July 25, 2013

Availability

Academic Institutions and Nonprofits only

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pFUGW-Pol2-ffLuc2-eGFP

Plasmid

#71394

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

Depositor

Glenn Merlino

Article

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

Pol2-ffLuc2-eGFP

Use

Lentiviral

Expression

Mammalian

Promoter

Pol2 (mouse RNA polymerase II gene promtoer)

Available Since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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hCas9-VPR

Plasmid

#68497

Purpose

nuclease competent SP-Cas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

SP-Cas9-VPR

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pICH41780

Plasmid

#48019

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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pLD564 human L1 ORF2p-3xFlag (L1RP, CMV promoter) in pCEP4 Puro

Plasmid

#213030

Purpose

Expresses full length human LINE-1 in human cells (native L1RP sequence), with a C-terminal 3xFlag tag on ORF2

Depositor

Martin S. Taylor

Article

Baldwin et al Nature. 2024 Feb;626(7997):194-206. doi:

Insert

Human LINE-1 (L1RP Sequence) with 5' UTR and ORF2-3xFlag

Tags

3xFlag (ORF2p)

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pU6_N7-sgRNA2

Plasmid

#190664

Purpose

Expresses U6-driven N7 sgRNA2 (poly-T mutated out), BsmbI dropout cassette for cloning

Depositor

Benjamin Kleinstiver

Article

Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

N7 sgRNA2

Expression

Mammalian

Promoter

U6

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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mEos3.2-N1

Plasmid

#54525

Purpose

Localization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson, Tao Xu

Article

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-Parkin

Plasmid

#23956

Purpose

Mammalian expression of human Park2 fused to mCherry

Depositor

Richard Youle

Article

Narendra et al J Cell Biol. 2008 Dec 1. 183(5):795-803.

Insert

Park2 (PRKN Human)

Tags

mCherry

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only

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pTRKH3-slpGFP

Plasmid

#27168

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

surface (S)-layer protein promoter region fused with modified green fluorescent protein 5

Expression

Bacterial

Available Since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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ER-P-TAG (pGS594)

Plasmid

#160039

Purpose

pLV-A4-UCOE-EF1a-CP450TMD-APEX2-3xHA-IRES-BLAS-2A-SNAP-WPRE-A2

Depositor

David Sabatini

Insert

CP450TMD-APEX2-3xHA

Use

Lentiviral

Promoter

EF1

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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FLAG-HA-pcDNA3.1-

Plasmid

#52535

Purpose

enables eukaryotic expression of N-terminally FLAG-HA-tagged fusion proteins

Depositor

Adam Antebi

Article

Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

FLAG-HA

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-TF

Plasmid

#83921

Purpose

Co-expresses cytoplasmic copies of the Skp and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
Trigger Factor

Expression

Bacterial

Promoter

T7

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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lenti-sgRNA blast

Plasmid

#104993

Purpose

This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and blasticidin S resistance from an EF-1a promoter.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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SpCas9-NG-SsAPOBEC3B

Plasmid

#198536

Purpose

Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD

Depositor

Hyongbum Kim

Article

Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

Insert

SpCas9-NG-SsAPOBEC3B

Use

CRISPR and Lentiviral

Tags

BSD, FLAG, and NLS

Expression

Mammalian

Promoter

EFS

Available Since

Nov. 5, 2024

Availability

Academic Institutions and Nonprofits only

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spNW30

Plasmid

#173485

Purpose

Expresses spNW30 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW30

Tags

Histag

Expression

Bacterial

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pLVX_DSB_Spectrum-V2

Plasmid

#192475

Purpose

Lentiviral DSB Spectrum V2 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, incompleteGFP

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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