171,965 results

Showing: 9851 - 9900 of 171965 results

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pAV0785

Plasmid

#133507

Purpose

Expression of mCherry tagged PCNA to monitor DNA replication

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pcn1)-mCherry-pcn1-terminator(nmt1)-natMX

Expression

Yeast

Available Since

Nov. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Jag1-HA pIRES

Plasmid

#17336

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Jagged1 (Jag1 Rat)

Tags

HA X3

Expression

Mammalian

Mutation

A silent C->G mutation at 3237

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only

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pFsynW KDEL Hook

Plasmid

#197277

Purpose

Encodes ER-targeted streptavidin-KDEL hook (RUSH system).

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

Streptavidin

Use

Lentiviral

Tags

FLAG

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

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pOpen-THEAQpolA

Plasmid

#165512

Purpose

DNA polymerase used in routine PCR method because of its high thermostability, standard testing, screening and output testing.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

Taq DNA Polymerase

Use

Synthetic Biology

Available Since

Aug. 9, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-GFP

Plasmid

#32395

Depositor

John T Gray

Article

Gray et al Methods Mol Biol. 2011;807:25-46.

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pCWX-UBI-hTert-PGK-BSD

Plasmid

#114316

Purpose

Constitutive expression of the hTert protein

Depositor

Patrick Salmon

Article

Salmon et al Methods Mol Biol. 2013;945:417-48. doi:

Insert

hTERT (TERT Human)

Use

Lentiviral

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) K-Ras(G12C)

Plasmid

#224284

Purpose

Cell transfection and expression of K-Ras (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Expression

Mammalian

Mutation

G12C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_HaloTag7_T2A_EGFP

Plasmid

#169325

Purpose

Expression of HaloTag7 in mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Type

Empty backbone

Expression

Mammalian

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pEBFP2

Plasmid

#165831

Purpose

EBFP2blue fluorescent protein CDS

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

EBFP2

Expression

Insect

Available Since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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UVR8-GFPCaaX

Plasmid

#49805

Purpose

expresses UVR8 fused to GFP with a prenylation motif for membrane targeting

Depositor

Matthew Kennedy

Article

Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

Insert

UVR8-GFPCaax (UVR8 Mustard Weed)

Expression

Mammalian

Available Since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pFP5370 ss-FIREmate-HA- KDEL-IVS-IRES- TNF-SBP- mCherry-FIREtag

Plasmid

#216696

Purpose

Synchronize trafficking of TNFalpha from the ER (RUCH system)

Depositor

Franck Perez

Article

Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

Inserts

FIREmate-KDEL
Tumor Necrosis Factor alpha (TNFa)

Tags

FIREtag, Streptavidin Binding Protein (SBP), and …

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCI-OR1A1

Plasmid

#22319

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

OR1A1 (OR1A1 Human)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pLOX-CW-CRE

Plasmid

#12238

Depositor

Didier Trono

Article

Cudre-Mauroux et al Hum Gene Ther. 2003 Nov 1. 14(16):1525-3

Insert

CRE

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Mutation

CMV promoter is upstream of nls-CRE. LoxP in 3&#x…

Available Since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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AAV_NLS-dSaCas9-NLS-VPR

Plasmid

#68495

Purpose

AAV vector containing nuclease null SaCas9 fused to VPR

Depositor

George Church

Article

Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

Insert

dSaCas9

Use

AAV

Tags

VPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pDDYFP-A

Plasmid

#40288

Depositor

Robert Campbell

Article

Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

Insert

ddYFP-A

Tags

His

Expression

Bacterial

Available Since

Sept. 17, 2012

Availability

Academic Institutions and Nonprofits only
hCtRNA_FT

Plasmid

#186715

Purpose

Fragment template for DAP array

Depositor

Xue Gao

Article

Yuan et al Nat Commun. 2022 May 19;13(1):2771. doi:

Insert

gRNA scaffold and hCtRNA

Use

CRISPR

Expression

Mammalian

Promoter

hCtRNA

Available Since

July 6, 2022

Availability

Academic Institutions and Nonprofits only

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dClover2-N1

Plasmid

#54538

Purpose

Localization: N1 Cloning Vector, Excitation: 505, Emission: 515. This plasmid encodes dClover2 A206K, H231L based on publication.

Depositor

Michael Davidson

Article

Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

Type

Empty backbone

Tags

dClover2

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pMIEG3-JunB

Plasmid

#40349

Depositor

Alexander Dent

Article

Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

Insert

JunB (Jun Mouse)

Use

Retroviral

Tags

IRES eGFP

Expression

Mammalian

Available Since

Oct. 15, 2012

Availability

Academic Institutions and Nonprofits only

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TFORF0801

Plasmid

#141707

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ATF3 (ATF3 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-mCherry-hPMCA2w/b

Plasmid

#111570

Purpose

Expresses a plasma membrane calcium pump hPMCA2w/b

Depositor

Baljit Khakh

Article

Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

Insert

Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)

Tags

mCherry

Expression

Bacterial

Available Since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBI-MCS-EGFP

Plasmid

#16542

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Type

Empty backbone

Expression

Mammalian

Available Since

June 11, 2008

Availability

Academic Institutions and Nonprofits only

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AAV-Best1-sCX3CL1

Plasmid

#164422

Purpose

AAV plasmid expressing soluble CX3CL1 (fractalkine) in retinal pigment epithelium

Depositor

Connie Cepko

Article

Wang et al Proc Natl Acad Sci U S A. 2019 May 14;11

Insert

Cx3cl1 (Cx3cl1 Mouse)

Use

AAV

Expression

Mammalian

Mutation

deleted terminal 59 amino acids

Promoter

Best1

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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mEGFP-PLIN3

Plasmid

#222426

Purpose

To express PLIN3 with mEGFP tag in mammalian cells

Depositor

Stephen Royle

Article

Downie et al PLoS Biol. 2025 Jul 10;23(7):e3003249. d

Insert

PLIN3 (PLIN3 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pinducer 20 DN-KASH

Plasmid

#125554

Purpose

Tet-ON inducible lentivirus expressing mcherry-labeled DN KASH

Depositor

Daniel Conway

Article

Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

Insert

SYNE1 (SYNE1 Human)

Use

Lentiviral

Tags

mcherry

Mutation

Truncated form: only the KASH domain of nesprin-…

Promoter

tetracycline responsive element

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pGSPE-ccdb

Plasmid

#242424

Purpose

for pegRNA cloning

Depositor

Caixia Gao

Article

Sun et al Cell. 2025 Aug 1:S0092-8674(25)00800-1.

Insert

ccdb

Use

CRISPR

Expression

Plant

Available Since

Dec. 17, 2025

Availability

Academic Institutions and Nonprofits only

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Flag-FOXL1

Plasmid

#153134

Purpose

Expression of Flag tagged human FOXL1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXL1 (FOXL1 Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV IE94

Available Since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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p53 (dominant negative R175H mutant)-pcw107-V5

Plasmid

#64638

Purpose

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

TP53 (transcript variant 3) (TP53 Human)

Use

Lentiviral

Tags

V5

Mutation

R175H

Promoter

PGK

Available Since

Dec. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pMY-IRES-eGFP

Plasmid

#163361

Purpose

Retroviral backbone for in-frame cloning of a gene of interest and an IRES separated GFP marker

Depositor

Louis Ates, Teunis Geijtenbeek

Article

van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

Jan. 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC57-TPNOX

Plasmid

#87853

Purpose

This plasmid contains human codon optimized sequence of Lactobacillus brevis mutant of a water-forming oxidase that can be used to increase NADP+/NADPH ratio in mammalian cells.

Depositor

Vamsi Mootha

Article

Cracan et al Nat Chem Biol. 2017 Aug 7. doi: 10.1038/

Insert

TPNOX

Tags

C-terminal FLAG tag with a linker

Expression

Bacterial

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-GluA2-Fab-LC

Plasmid

#230063

Purpose

Expresses light chain Fab fragment of anti-GluA2. Can be used with heavy chain to produce Fab in HEK cells.

Depositor

Matthew Kennedy

Insert

Anti-GluA2 Fab light chain fragment

Expression

Mammalian

Promoter

Chicken beta actin (CAG)

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLVX-IRES-hygro-EGFP-Stx17

Plasmid

#174652

Purpose

Vector for overexpression of EGFP-tagged Stx17 with IRES for hygromycin selection.

Depositor

Lukas Kapitein

Article

Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

Insert

EGFP-Stx17 (STX17 Human)

Use

Lentiviral

Tags

EGFP

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK5_mCherry_P2AT2A_GFP-nb-KS

Plasmid

#238233

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pBA560

Plasmid

#186236

Purpose

pTet-eLbuCas13a crRNA entry vector

Depositor

Jennifer Doudna

Article

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Expression

Bacterial

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
hKCNQ1 pCDNA3.1(-)

Plasmid

#219953

Purpose

Expresses human KCNQ1 in mammalian cells

Depositor

Niels Decher

Article

Kiper et al Proc Natl Acad Sci U S A. 2024 Jun 18;12

Insert

KCNQ1 (KCNQ1 Human)

Expression

Mammalian

Mutation

contains silent sequence variations

Promoter

CMV

Available Since

Aug. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pBEN276

Plasmid

#69150

Purpose

Integration of luciferase operon in Tn7 insertion site of Gram negative bacteria

Depositor

Pierre Germon

Article

Howe et al BMC Microbiol. 2010 Jul 23;10:197. doi:

Insert

luxCDABE

Use

Luciferase

Promoter

frr

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
LBR-mCherry

Plasmid

#172446

Purpose

Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

Depositor

Stephen Royle

Article

Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

lamin B receptor (LBR Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe_Hyg_cOVA_T2A_mStrawberry

Plasmid

#161737

Purpose

Expression of cOVA and mStrawberry

Depositor

Alec Kimmelman

Article

Yamamoto et al Nature. 2020 May;581(7806):100-105. doi:

Insert

cytoplasmic ovalbumin

Use

Retroviral

Tags

mStrawberry

Expression

Mammalian

Available Since

Dec. 21, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
tetO-ASCL1-LMX1b-NURR1-PuroR

Plasmid

#182298

Purpose

Doxycycline-inducible vector expressing the ALN transcription factors and PuroR (all separated by 2A sequences)

Depositor

Kristen Brennand

Article

Powell et al Mol Psychiatry. 2021 Sep 7. pii: 10.1038

Insert

ASCL1-2A-LMX1B-2A-NURR1 (ASCL1, LMX1B Human)

Use

Lentiviral

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pBP-split-intein-Gal4[1-20]-N-int

Plasmid

#199201

Purpose

Gateway destination vector for enhancer-driven split-intein Gal4[1-20]N-int expression

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

split-intein-Gal4[1-20]-N-int

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
p3xnls-mScarlet3_N1

Plasmid

#189775

Purpose

Construct for labelling cell nuclei in mammalian cells using nuclear targeted mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

3xnls-mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag p21 WT

Plasmid

#16240

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2001 Mar . 3(3):245-52.

Insert

p21 (CDKN1A Human)

Tags

Flag

Expression

Mammalian

Available Since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW COX8ASig-mCherry

Plasmid

#239558

Purpose

Expression and mitochondrial localization of mCherry

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

mCherry

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ER-RspA-NFAST

Plasmid

#233595

Purpose

Expression of RspA-NFAST on the ER membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

RspA-NFAST

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
WRN scFv [N428/12]

Plasmid

#206784

Purpose

Mammalian Expression of WRN scFV. Derived from hybridoma N428/12 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

WRN (Homo sapiens) recombinant scFV (WRN Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDR119

Plasmid

#13332

Depositor

Martin Gellert

Article

Modesti et al EMBO J. 1999 Apr 1. 18(7):2008-18.

Insert

DNA ligase IV (LIG4 Human)

Tags

His

Expression

Bacterial

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only
mTFP1-N1

Plasmid

#54521

Purpose

Localization: N1 Cloning Vector, Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Type

Empty backbone

Tags

mTFP1

Expression

Mammalian

Available Since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
mNeonGreen-KDEL

Plasmid

#226568

Purpose

Encodes mNeonGreen with KDEL ER lumenal target sequence

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

KDEL (ER lumenal target sequence)

Tags

mNeonGreen

Expression

Mammalian

Available Since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pOZ-N-FH RAP80

Plasmid

#27501

Depositor

Roger Greenberg

Article

Patterson-Fortin et al J Biol Chem. 2010 Oct 1. 285(40):30971-8

Insert

RAP80 (UIMC1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Available Since

March 1, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pYH80

Plasmid

#217387

Purpose

Ptrc99A-mCherry-L78-PopTag for bacterial expression

Depositor

Anthony Vecchiarelli

Article

Hoang et al Nat Commun. 2024 Apr 15;15(1):3222. doi:

Insert

PopTag

Tags

mCherry-Linker78

Expression

Bacterial

Available Since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-CAG-Cre

Plasmid

#196140

Purpose

AAV-mediated expression of Cre under the CAG promoter .

Depositor

Edward Boyden

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Cre

Use

AAV

Expression

Mammalian

Mutation

codon diversified

Promoter

CAG

Available Since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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171,965 results