4,180 results

1. Cdc2-DN-HA

   Plasmid

   #1889

   Depositor

   Sander van den Heuvel

   Article

   van den Heuvel et al Science 1993 Dec 24;262(5142):2050-4.

   Insert

   Cdc2 (CDK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D146N, dominant negative

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-hSyn1-mRuby2-GSG-P2A-GCaMP6s-WPRE-pA

   Plasmid

   #50942

   Purpose

   Bicistronic vector expressing mRuby2 and GCaMP6s from a single open reading frame.

   Depositor

   Mark Huebener, Tobias Rose, Tobias Bonhoeffer

   Article

   Rose et al Science. 2016 Jun 10;352(6291):1319-22.

   Has Service

   AAV1

   Insert

   mRuby2-P2A-GCaMP6s
   

   Use

   AAV

   Promoter

   hSyn1

   Available Since

   March 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG-mir30

   Plasmid

   #14758

   Depositor

   Connie Cepko

   Article

   Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

   Insert

   mir30 cassette
   

   Use

   RNAi

   Expression

   Mammalian

   Available Since

   April 20, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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4. 3xFLAG-dCas9/pMXs-puro

   Plasmid

   #51240

   Purpose

   Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

   Depositor

   Hodaka Fujii

   Article

   Fujita et al PLoS One. 2014 Jul 22;9(7):e103084. doi:

   Insert

   3xFLAG-dCas9
   

   Use

   CRISPR and Retroviral

   Tags

   3xFLAG tag and NLS (nuclear localization signal)

   Expression

   Mammalian

   Mutation

   human codon-optimized, D10A + H840A

   Promoter

   LTR

   Available Since

   Aug. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

   Plasmid

   #41863

   Purpose

   This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

   Depositor

   Feng Zhang

   Article

   Cong et al Science. 2013 Jan 3.

   Insert

   U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   Feb. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEGFP-PCNA-IRES-puro2b

   Plasmid

   #26461

   Depositor

   Daniel Gerlich

   Article

   Held et al Nat Methods. 2010 Sep . 7(9):747-54.

   Insert

   PCNA (PCNA Human)
   

   Tags

   pEGFP

   Expression

   Bacterial and Mammalian

   Available Since

   Dec. 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMXs-c-Myc-IP

   Plasmid

   #15921

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   myelocytomatosis oncogene (Myc Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Feb. 8, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. OCT4-eGFP-2A-Puro

   Plasmid

   #31939

   Depositor

   Rudolf Jaenisch

   Article

   Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

   Inserts

   OCT4 (POU5F1 Human)
   eGFP (eGFP )
   

   Use

   Targeting donor

   Tags

   eGFP

   Available Since

   Sept. 13, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Tag4A-MITF-M (wt)

   Plasmid

   #31151

   Depositor

   Yardena Samuels

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   MITF-M (MITF Human)
   

   Tags

   FLAG

   Expression

   Bacterial and Mammalian

   Mutation

   WT

   Available Since

   July 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-f:PGC1b

    Plasmid

    #1031

    Depositor

    Bruce Spiegelman

    Article

    Lin et al J Biol Chem 2002 Jan 18;277(3):1645-8. E

    Insert

    PGC-1 beta (Ppargc1b Mouse)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    Oct. 4, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSQT834

    Plasmid

    #53371

    Purpose

    Csy4 and Cas9 nuclease expression plasmid

    Depositor

    Keith Joung

    Article

    Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Csy4-T2A-Cas9-NLS
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid

    #48226

    Purpose

    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    

    Use

    CRISPR

    Tags

    2A-puro, HA Tag, and VP160

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available Since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEZYegfp

    Plasmid

    #18671

    Purpose

    pEGFPC4 converted Gateway Destination vector for expressing EGFP tagged fusion proteins in mammalian cells

    Depositor

    Yu-Zhu Zhang

    Article

    Guo et al Biotechnol J. 2008 Mar . 3(3):370-7.

    Type

    Empty backbone

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLAG-FOXO3a TM

    Plasmid

    #8361

    Depositor

    Michael Greenberg

    Article

    Brunet et al Science 2004 Mar 26;303(5666):2011-5. Ep

    Insert

    FOXO3a (FOXO3 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    T32A, S253A, S315A

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEGFP-NMHC II-C

    Plasmid

    #10843

    Depositor

    Robert Adelstein

    Article

    Golomb et al J Biol Chem. 2004 Jan 23. 279(4):2800-8.

    Insert

    NMHC II-C (Myh14 Mouse)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVPT-rtTR-KRAB-2SM2

    Plasmid

    #11652

    Purpose

    Tet-regulated (Tet-off) lentiviral vector for transgene (mPGK promoter) - AND/OR - shRNA (H1 promoter when subcloned from pLVTHM (Addgene#12247)) - 2nd generation

    Depositor

    Patrick Aebischer, Didier Trono

    Article

    Szulc et al Nat Methods. 2006 Feb;3(2):109-16. doi:

    Insert

    mPGK, GFP, rtTR-KRAB-2SM2, Tet-off
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 17, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS424GFP-FYVE(EEA1)

    Plasmid

    #36096

    Depositor

    Scott Emr

    Article

    Burd et al Mol Cell. 1998 Jul;2(1):157-62.

    Insert

    FYVE(EEA1) domain (EEA1 Human)
    

    Tags

    GFP

    Expression

    Yeast

    Promoter

    PRC1

    Available Since

    June 14, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFRT/TO/FLAG/HA-DEST DICER

    Plasmid

    #19881

    Depositor

    Thomas Tuschl

    Article

    Landthaler et al RNA. 2008 Dec;14(12):2580-96. doi: 10.12

    Insert

    Dicer 1, ribonuclease type III (DICER1 Human)
    

    Tags

    FLAG/HA

    Expression

    Mammalian

    Available Since

    Dec. 31, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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19. YFP-FKBP (YF)

    Plasmid

    #20175

    Depositor

    Tobias Meyer

    Article

    Inoue et al Nat Methods. 2005 Jun . 2(6):415-8.

    Insert

    YFP-FKBP (YF): (FKBP1A Human)
    

    Expression

    Mammalian

    Mutation

    FKBP was taken out from pC4M-F2E with a flanking …

    Available Since

    March 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA cyclin D1 HA

    Plasmid

    #11181

    Depositor

    Bruce Zetter

    Article

    Newman et al J Biol Chem. 2004 Oct 1. 279(40):41504-1

    Insert

    cyclin D1 (CCND1 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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