We narrowed to 6,963 results for: RAP

1. Pet22b-YAPL318E

   Plasmid

   #166444

   Purpose

   Bacterial expression of 6x His tagged YAPL318E

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   YAPL318E (YAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. Pet22b-YAP4LE

   Plasmid

   #166445

   Purpose

   Bacterial expression of 6x His tagged YAP4LE

   Depositor

   Shuguo Sun

   Article

   Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

   Insert

   YAP4LE (YAP1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQC V5 mKO2 HuR.DelHNS IRES Puro

   Plasmid

   #110389

   Purpose

   γ-Retroviral transfer vector for expressing HuR (delHNS), V5 and mKO2 tags, IRES-driven Puromycin selection.

   Depositor

   Sandra Martha Gomes Dias

   Insert

   ELAV like RNA binding protein 1 (ELAVL1 Human)
   

   Use

   Retroviral

   Tags

   V5 and mKO2

   Expression

   Mammalian

   Mutation

   HNS (HuR nuclear-cytoplasmic shuttling sequence) …

   Promoter

   CMV

   Available since

   Feb. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. S1 1029 CP APOBEC3 no UGI

   Plasmid

   #135352

   Purpose

   Expression of circular permutant of nSpyCas9 lacking uracil DNA glycosylase inhibitor at amino acid position 1029 encoding rat cytosine deaminase, rAPOBEC3 at the N-terminus

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   rAPOBEC3-nSpyCas9 aa 1029
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. AAVS1 intron 1 gRNA as2

   Plasmid

   #132394

   Purpose

   Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223 ATG3 C264A

   Plasmid

   #129293

   Purpose

   Gateway entry clone encoding human ATG3 C264A (catalytic inactive)

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Gateway entry vector / entry clone

   Tags

   Expression

   Mutation

   changed Cysteine 264 to Alanine

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDONR223 ATG3 allKR

   Plasmid

   #129295

   Purpose

   Gateway entry clone encoding human ATG3 with all 22 lysine residues mutated to arginine

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Gateway entry vector / entry clone

   Tags

   Expression

   Mutation

   changed all 22 Lysine residues to Arginine

   Promoter

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV 3xFLAG-ATG3 allKR

   Plasmid

   #129298

   Purpose

   Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed all 22 Lysine residues to Arginine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV 3xFLAG-ATG3 1-13 KR

   Plasmid

   #129299

   Purpose

   Expresses pCMV 3xFLAG-ATG3 1-13 KR (first thirteen lysines mutated to arginine) in mammalian cells

   Depositor

   Robin Ketteler

   Article

   Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

   Insert

   Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed first 13 Lysine residues to Arginine

   Promoter

   CMV

   Available since

   Nov. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV 3xFLAG-ATG3 14-22 KR

    Plasmid

    #129300

    Purpose

    Expresses pCMV 3xFLAG-ATG3 14-22 KR (last nine lysines mutated to arginine) in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed last 9 Lysine residues to Arginine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV 3xFLAG-ATG3 K295R

    Plasmid

    #129302

    Purpose

    Expresses pCMV 3xFLAG-ATG3 K295R in mammalian cells

    Depositor

    Robin Ketteler

    Article

    Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

    Insert

    Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Lysine 295 to Arginine

    Promoter

    CMV

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

    Plasmid

    #120295

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLKO.1-TRC.mKO2_shHRASLS.2

    Plasmid

    #110324

    Purpose

    TRCN0000002620 (Target GCGATAAGTACCGTTGAGTTT), silence human HRASLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    HRASLS (PLAAT1 Homo sapiens, Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLKO.1-TRC.mKO2_shHRASLS.1

    Plasmid

    #110323

    Purpose

    TRCN0000378630 (Target GATAAGTACCGTTGAGTTTG), silence human HRASLS gene and express monomeric Kusabira-Orange2

    Depositor

    Sandra Martha Gomes Dias

    Article

    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1

    Insert

    HRASLS (PLAAT1 Homo sapiens, Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    RNA polymerase III promoter for human U6 snRNA fo…

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Adeno BN

    Plasmid

    #101823

    Purpose

    Adenovirus for the expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1

    Depositor

    Andrea Ventura

    Article

    Cook et al Nat Commun. 2017 Jul 11;8:15987. doi: 10

    Insert

    U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)_CBh_FLAG-Cas9 (Bcan, Ntrk1 Mouse)
    

    Use

    Adenoviral

    Tags

    FLAG-Cas9

    Expression

    Mutation

    Promoter

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pETM6-1D4-mCherry

    Plasmid

    #73423

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1D4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1D4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1D4 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETM6-4A6-mCherry

    Plasmid

    #73424

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4A6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4A6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P4A6 (orthogonal T7-lac variant)

    Available since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM6-1E4-mCherry

    Plasmid

    #73426

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1E4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1E4 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1E4 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pETM6-1B6-mCherry

    Plasmid

    #73420

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 1B6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 1B6 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P1B6 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pETM6-3H5-mCherry

    Plasmid

    #73419

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3H5.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 3H5 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    P3H5 (orthogonal T7-lac variant)

    Available since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pde3b_L (OZ591)

    Plasmid

    #35231

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting pde3b (pde3b Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pde3b_R (OZ592)

    Plasmid

    #35232

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting pde3b (pde3b Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

23. LOC566040_ R (OZ588)

    Plasmid

    #35228

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting LOC566040 (LOC566040 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

24. fam82a2_L (OZ573)

    Plasmid

    #35213

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting fam82a2 (rmdn3 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only

25. LOC566040_ L (OZ587)

    Plasmid

    #35227

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting LOC566040 (LOC566040 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

26. morc3b_R (OZ576)

    Plasmid

    #35216

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting morc3b (morc3b Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

27. fam82a2_R (OZ574)

    Plasmid

    #35214

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting fam82a2 (rmdn3 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

28. morc3b_L (OZ575)

    Plasmid

    #35215

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting morc3b (morc3b Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 5, 2012

    Availability

    Academic Institutions and Nonprofits only

29. xylt1_R (OZ510)

    Plasmid

    #27185

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting xylt1 (xylt1 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

30. pitrm1_R (OZ516)

    Plasmid

    #27191

    Depositor

    Keith Joung

    Article

    Maeder et al Mol Cell. 2008 Jul 25. 31(2):294-301.

    Insert

    Zinc finger array targeting pitrm1 (pitrm1 Zebrafish)
    

    Use

    Zebrafish targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only