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pMS5: pHelper(AvCAST)_entry_ΔTniQ

Plasmid

#168138

Purpose

Inducible expression of AvCAST proteins (except TniQ). Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB and TnsC)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pMS4: pHelper(AvCAST)_entry

Plasmid

#168137

Purpose

Inducible expression of AvCAST proteins. Two BsaI sites for spacer cloning.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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D76_Ld_303190

Plasmid

#135889

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Translocon-associated protein beta (TRAPB), putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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D62_Ld_362720

Plasmid

#135875

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

membrane-bound acid phosphatase 2, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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D46_Ld_362700

Plasmid

#135860

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

membrane-bound acid phosphatase precursor

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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D74_Ld_291730

Plasmid

#135887

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

endosomal integral membrane protein, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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D83_Ld_060380

Plasmid

#135896

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

Present in the outer mitochondrial membrane proteome 35

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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D9_Ld_190920

Plasmid

#135825

Purpose

Expression of Leishmania donovani ectodomain in mammalian cells

Depositor

Gavin Wright

Article

Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

Insert

peptidyl-prolyl cis-trans isomerase, macrophage infectivity potentiator precursor, putative

Tags

biotinylation sequence and His tag

Expression

Mammalian

Available Since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

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pLuc-CDS-m640-1+2

Plasmid

#42106

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Silent mutations were introduced in two seed-comp…

Promoter

SV40

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLuc-CDS-m640-2

Plasmid

#42105

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Silent mutations were introduced in the seed-comp…

Promoter

SV40

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLuc-CDS-m215-1+2

Plasmid

#42103

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Silent mutations were introduced in two seed-comp…

Promoter

SV40

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pLuc-CDS-m640-1

Plasmid

#42104

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Silent mutations were introduced in the seed-comp…

Promoter

SV40

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pLuc-CDS-m215-1

Plasmid

#42101

Depositor

Kumiko Ui-Tei

Article

Mazda et al PLoS One. 2011;6(12):e28688. doi: 10.137

Insert

ZEB1 (ZEB1 Human)

Use

Luciferase

Tags

Renilla luciferase

Expression

Mammalian

Mutation

Silent mutations were introduced in the seed-comp…

Promoter

SV40

Available Since

March 6, 2013

Availability

Academic Institutions and Nonprofits only

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AiP11457 - pAAV-eHGT_078h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1457)

Plasmid

#163495

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AiP2048 - pAAV-AiE2543m-minBG-iCre(R297T)-BGHpA

Plasmid

#220727

Purpose

AiE2543m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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AiP20027 - pAAV-AiE2182m_3xC2-minBG-iCre(R297T)-BGHpA

Plasmid

#220735

Purpose

AiE2182m_3xC2 is an optimized enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP2141 - pAAV-AiE2333m-minBG-iCre(R297T)-BGHpA

Plasmid

#220731

Purpose

AiE2333m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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AiP14532-pAAV-DLX2.0-minBG-FlpO-WPRE3-BGHpA (Alias: CN4532)

Plasmid

#222939

Purpose

FlpO recombinase expression from the DLX2.0 enhancer

Depositor

Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

FlpO (flp Synthetic)

Use

AAV

Available Since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AiP1795 - pAAV-AiE0170h-minBG-iCre(R297T)-BGHpA

Plasmid

#220717

Purpose

AiE0170h is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Bosiljka Tasic

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

iCre(R297T)

Use

AAV

Promoter

minBG

Available Since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
AiP11244 - pAAV-hDLXI56i-minBG-SYFP2-WPRE3-BGHpA (Alias: CN1244)

Plasmid

#163493

Purpose

Direct-expressing SYFP2 AAV Virus

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBetaGlobin

Available Since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge