We narrowed to 165,221 results for: addgene

1. pCY5n

   Plasmid

   #160296

   Purpose

   Yeast CRISPR plasmid targeting the natMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting nat: GTACCGCACCAGTGTCCCGG)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCY5k

   Plasmid

   #160294

   Purpose

   Yeast CRISPR plasmid targeting the kanMX cassette

   Depositor

   Amir Aharoni

   Article

   Soreanu et al Curr Genet. 2018 Oct;64(5):1129-1139. do

   Inserts

   sgRNA expression cassette (with guide targeting kan: TGTTTTGCCGGGGATCGCAG)
   URA3 selection cassette
   sgRNA expression cassette (without guide)
   Cas9
   

   Use

   CRISPR

   Tags

   8xHis-tag and SV40 NLS

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHW1133

   Plasmid

   #229869

   Purpose

   Destination RMCE vector containing FRT::SA::51-bp Artificial exon::3xStop::SL2::SapI Insertion sites (to clone new driver with 3'UTR in slot 5 and SEC in slot 6)::reverse FRT3 to swap driver via RMCE

   Depositor

   Han Wang

   Article

   Khan et al PLoS Genet. 2025 Jan 22;21(1):e1011541.

   Type

   Empty backbone

   Expression

   Worm

   Available Since

   Feb. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. N-Terminal Halo XRCC4

   Plasmid

   #207541

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous XRCC4 locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with internal PuroR cassette flanked by human XRCC4 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. N-terminal Halo-Ku70

   Plasmid

   #207547

   Purpose

   Homologous recombination donor to insert halo tag at the N terminal of the endogenous Ku70 locus.

   Depositor

   Jens Schmidt

   Article

   Mikhova et al Nat Commun. 2024 Nov 23;15(1):10159. doi

   Insert

   HaloTag with flanked by human Ku70 locus sequences
   

   Tags

   HaloTag

   Expression

   Mammalian

   Available Since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. aTY171_pPGK1_iRFP_WPRE

   Plasmid

   #225351

   Purpose

   Lentiviral vector for iRFP expression

   Depositor

   Wendell Lim

   Article

   Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

   Insert

   iRFP670
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. aTY173_pPGK1_mTagBFP2_WPRE

   Plasmid

   #225352

   Purpose

   Lentiviral vector for BFP expression

   Depositor

   Wendell Lim

   Article

   Yamada et al Cell. 2024 Dec 11:S0092-8674(24)01323-0.

   Insert

   BFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Dec. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-Puro-2xMS2

   Plasmid

   #212627

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-2xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-Puro-12xMS2

   Plasmid

   #212628

   Purpose

   MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

   Depositor

   Paul Blainey

   Article

   Najia et al bioRxiv 2024.10.01.616098

   Insert

   EF1a-PuroR-12xMS2-WPRE-hU6-sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-Puro-4xMS2

    Plasmid

    #212626

    Purpose

    MS2 hairpin-containing expression vector and sgRNA entry plasmid for the directed export of barcode transcripts in Gag-MCP virus-like particles

    Depositor

    Paul Blainey

    Article

    Najia et al bioRxiv 2024.10.01.616098

    Insert

    EF1a-PuroR-4xMS2-WPRE-hU6-sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pZB573_pB1H1_T7-RNAP_LARP-I5CHO_1

    Plasmid

    #228508

    Purpose

    Bacterial Hybrid Driver Plasmid

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    T7 RNAP, LARP-I5CHO (#1)
    

    Use

    Synthetic Biology

    Tags

    Umu Degron, GTG-start codon

    Mutation

    R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pZB578_pB1H1_T7-RNAP_LARP-I

    Plasmid

    #228507

    Purpose

    Bacterial Hybrid Driver Plasmid

    Depositor

    Timothy Whitehead

    Article

    Baumer et al ACS Synth Biol. 2024 Dec 20;13(12):4165-

    Insert

    T7 RNAP, LARP-I
    

    Use

    Synthetic Biology

    Tags

    Umu Degron, GTG-start codon

    Mutation

    R378K; S430P, N433T, S633P, F849I, F880Y; W727G, …

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX458-PLK4

    Plasmid

    #227310

    Purpose

    Expresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of PLK4 (PLK4 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p8270 LentiCRISPR v2 hygro sgSIGIRR-3

    Plasmid

    #193979

    Purpose

    Expression of spCas9 and sgRNA targeting SIGIRR

    Depositor

    Elizabeth White

    Article

    Castagnino et al J Virol. 2022 Nov 7:e0132622. doi: 10.11

    Insert

    spCas9 and sgRNA targeting SIGIRR (SIGIRR Human)
    

    Use

    Lentiviral

    Available Since

    Nov. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pU6-tevopreq1-FXR1-G266E

    Plasmid

    #225482

    Purpose

    Express epegRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    epegRNA for editing FXR1-G266E (FXR1 Human)
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    Oct. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. LsgRNA-FXR1-KH1-gRNA

    Plasmid

    #225483

    Purpose

    Express gRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    gRNA for editing FXR1-KH1 (FXR1 Human)
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. BPK1520-FXR1-N202S-gRNA

    Plasmid

    #225481

    Purpose

    Express gRNA for FXR1

    Depositor

    Christine Mayr

    Article

    Chen et al Cell. 5 August 2024. doi: 10.1016/j.cell

    Insert

    gRNA for editing FXR1-N202S (FXR1 Human)
    

    Use

    CRISPR

    Promoter

    hU6

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVUT-eC3GFP

    Plasmid

    #225485

    Purpose

    Tet-on lentiviral vector for controlled expression of C. botulinum exoenzyme C3 transferase and EGFP

    Depositor

    Robert Gross

    Article

    Gutekunst et al Neuroscience. 2016 Dec 17;339:308-318. d

    Insert

    Exoenzyme C3 transferase
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Amino acids 30-251, See Depositor Comments

    Promoter

    Human ubiquitin C

    Available Since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pST39-Mega'

    Plasmid

    #216877

    Purpose

    Derived from the plasmid for Mega GVs, pST39-pNL29 (Addgene #91696), but the overlap between GvpS and GvpK has been resolved.

    Depositor

    George Lu

    Article

    Iburg et al EMBO J. 2024 Oct;43(19):4156-4172. doi:

    Insert

    Derived from Mega, and GvpS and GvpK overlap has been resolved
    

    Expression

    Bacterial

    Available Since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p8391 LentiCRISPRv2 Neo sgPTPN14-1

    Plasmid

    #221651

    Purpose

    Expression of sgRNA targeting the locus of human PTPN14

    Depositor

    Elizabeth White

    Article

    Blakely et al mBio. 2024 Sep 9:e0181124. doi: 10.1128/

    Insert

    sgPTPN14-1 (PTPN14 Human)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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