2,226 results

1. pMK289 (mAID-mClover-NeoR)

   Plasmid

   #72827

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Neo
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. mTq2-Calmodulin

   Plasmid

   #198200

   Purpose

   FRET donor: Calmodulin-2 tagged with N-terminal mTurquoise2

   Depositor

   Christian Wahl-Schott

   Article

   Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

   Insert

   Calmodulin 2 (Calm2 Rat)
   

   Use

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. Phi3-GCaMP6f

   Plasmid

   #106298

   Purpose

   CMV driven GFP based calcium (Ca2+) reporter

   Depositor

   Sanford Simon

   Article

   Belote et al J Cell Biol. 2020 Jan 6;219(1). pii: 132

   Insert

   GCaMP6f
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCUP1-GR

   Plasmid

   #1212

   Depositor

   Susan Lindquist

   Article

   Li et al Science 2000 Jan 28;287(5453):661-4.

   Insert

   glucocorticoid receptor (Nr3c1 Rat)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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5. Z-lock cofilin

   Plasmid

   #141137

   Purpose

   controlling the actin-severing ability of cofilin with light

   Depositor

   Klaus Hahn

   Article

   Stone et al Nat Chem Biol. 2019 Dec;15(12):1183-1190

   Insert

   Zdk2, Cofilin, LOV2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   mCherry, Zdk2(132F), Cofilin S3A, LOV WT

   Promoter

   Available since

   Dec. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ptfLC3

   Plasmid

   #21074

   Purpose

   Mammalian expression of rat LC3 fused to mRFP and EGFP

   Depositor

   Tamotsu Yoshimori

   Article

   Kimura et al Autophagy. 2007;3(5):452-60.

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Use

   Tags

   EGFP and mRFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 28, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGP-CMV-GCaMP6m

   Plasmid

   #40754

   Purpose

   Mammalian expression of ultrasensitive protein calcium sensor

   Depositor

   Douglas Kim, GENIE Project

   Article

   Chen et al Nature. 2013 Jul 18;499(7458):295-300. d

   Insert

   GCaMP6m
   

   Use

   Tags

   6xHis, T7 epitope, and Xpress tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP_TRPM8

   Plasmid

   #64879

   Purpose

   Expresses Ion Channel TRPM8 fused to eGFP in mammalian cells

   Depositor

   Sebastián Brauchi

   Article

   Toro et al J Neurosci. 2015 Jan 14;35(2):571-82. do

   Insert

   Transient receptor potential cation channel subfamily M member 8 (Trpm8 Rat)
   

   Use

   Tags

   eGFP

   Expression

   Mutation

   Promoter

   Available since

   June 13, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CRY2-GFP-homer1c

   Plasmid

   #89442

   Purpose

   Localizes CRY2 to excitatory synapses

   Depositor

   Matthew Kennedy

   Article

   Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

   Insert

   CRY2-GFP-homer1c
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Contains Full length CRY2

   Promoter

   CAG

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCh-GluA1-CIB

    Plasmid

    #89444

    Purpose

    Allows conditional recruitment of AMPA receptors to the postsynaptic membrane

    Depositor

    Matthew Kennedy

    Article

    Sinnen et al Neuron. 2017 Feb 8;93(3):646-660.e5. doi

    Insert

    mCh-GluA1-CIB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CaMKII

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSIL-eGFP-shACTN1

    Plasmid

    #52676

    Purpose

    shRNA against α-actinin-1 with eGFP transfection marker

    Depositor

    Johannes Hell

    Article

    Schnizler et al J Biol Chem. 2009 Jan 30;284(5):2697-705

    Insert

    ACTN1 (Actn1 Rat)
    

    Use

    RNAi

    Tags

    CMV-EGFP

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMRX-IP-GFP-LC3-RFP-LC3ΔG

    Plasmid

    #84572

    Purpose

    Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

    Insert

    microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
    

    Use

    Retroviral

    Tags

    EGFP and mRFP1

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3/F-tractin=mStayGold

    Plasmid

    #212019

    Purpose

    For filamentous actin labeling. Alternatively, the F-tractin gene can be replaced with a target molecule gene for C-terminal tagging with mStayGold through a linker [(GGGGS)3] (denoted as ‘=’).

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    F-tractin
    

    Use

    Tags

    mStayGold

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMRX-No-HaloTag7-mGFP-LC3-mRFP

    Plasmid

    #184902

    Purpose

    Stable expression of HaloTag7-mGFP-LC3-mRFP in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7, monomeric EGFP, and monomeric RFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. mClY-N1

    Plasmid

    #90457

    Purpose

    A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

    Depositor

    Joseph Santos-Sacchi

    Article

    Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

    Insert

    mClY-N1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

    Promoter

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMRX-IP-HaloTag7-LC3

    Plasmid

    #184899

    Purpose

    Stable expression of HaloTag7-LC3 in mammalian cells to measure autophagic flux

    Depositor

    Noboru Mizushima

    Article

    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1

    Insert

    LC3B
    

    Use

    Tags

    HaloTag7

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FUdeltaGW-rtTA

    Plasmid

    #19780

    Purpose

    3rd generation lentiviral vector

    Depositor

    Konrad Hochedlinger

    Article

    Maherali et al Cell Stem Cell. 2008 Sep 11. 3(3):340-5.

    Insert

    reverse tetracycline transactivator
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 24, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEAK10-His-Myc-Kal12

    Plasmid

    #25442

    Depositor

    Betty Eipper

    Article

    Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

    Insert

    Kal12
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

19. cacna2d1 pCAGGS

    Plasmid

    #206104

    Purpose

    expression of rat alpha2delta-1 calcium channel auxillary subunit

    Depositor

    Annette Dolphin

    Insert

    cacna2d1 (Cacna2d1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/B-actin

    Available since

    Nov. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pEAK10-His-Myc-Kal9

    Plasmid

    #25441

    Depositor

    Betty Eipper

    Article

    Johnson et al J Biol Chem. 2000 Jun 23. 275(25):19324-

    Insert

    Kal9
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only