-
Plasmid#182944PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein can bind all 3 TRK receptors and p75NTRDepositorInsertMNTS-1 (NTF3 Homo sapiens, Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationThis is the Human NT-3 with mutations on the matu…PromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDF-NtXNtS1HR1AtR2NtB2
Plasmid#160890PurposeExpression of N. tabacum Rubisco small subunit (rbcS-SH1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.DepositorInsertPT7-Nt-rbcS-S1H
UseTags6xHisExpressionBacterialMutationPromoterrbcS-SH1: T7, chaperones: T13Available sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-INTS3-C
Plasmid#128642PurposeExpress INTS3 C-termini (514-1042 aa) in E. coli with N-terminal and C-terminal His tagDepositorInsertINTS3 C-termini (INTS3 Human)
UseTagsHis tag on N and C-terminalExpressionBacterialMutationINTS3 C-termini (514–1042 aa)PromoterT7Available sinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
-
pRRL-VinculinTS-I997A
Plasmid#111832PurposeVinculin tension sensor with point mutation affecting ability to bind actin in lentiviral expression vectorDepositorInsertVinculinTS (I997A) (VCL Chicken)
UseLentiviralTagsExpressionMutationchanged vinculin isoleucine 997 to alaninePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS9
Plasmid#111765PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)9-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)9 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinculinTS-A50I/I997A
Plasmid#111829PurposeVinculin tension sensor with point mutations affecting ability to bind talin and actinDepositorInsertVinculinTS (I997A)
UseTagsExpressionMammalianMutationchanged vinculin alanine 50 to isoleucine and iso…PromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS8 AA 1-308
Plasmid#196905PurposeExpresses His-tagged Drosophila IntS8 amino acids 1-308DepositorInsertIntegrator subunit 8 (IntS8 Fly)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS7
Plasmid#111764PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)7-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)7 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-A50I
Plasmid#111831PurposeVinculin tension sensor with point mutation affecting ability to bind talin in lentiviral expression vectorDepositorInsertVinculinTS (A50I) (VCL Chicken)
UseLentiviralTagsExpressionMutationchanged vinculin alanine 50 to isoleucinePromoterCMVAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
VinTS-tCRMod-GGSGGS5
Plasmid#111763PurposeExpresses vinculin tension sensor comprised of Clover-(GGSGGS)5-mRuby2 moduleDepositorInsertClover-mRuby2-based vinculin tension sensor with (GGSGGS)5 extensible domain
UseTagsExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
PGL4.11-COL1A1double mutants
Plasmid#230918PurposeDouble mutation of TEAD and SMAD binding elements in COL1A1 promoter; check the promoter activity.DepositorInsertHuman CLO1A1 promoter including SMAD and TEAD mutant elements (COL1A1 Human)
UseLuciferaseTagsLuciferase-luc2pExpressionBacterial and MammalianMutationCOL1A1 promoter with mutant SMAD and TEAD binding…PromoterCOL1A1 with mutant SMAD and TEAD elementsAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1015A
Plasmid#213415PurposeVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1015A).DepositorInsertVcnTS-E1015A (VCL Chicken, Synthetic)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1021A
Plasmid#213416PurposeVinculin tension sensor (VcnTS) with a single directionally asymmetric, force strengthening (DAFS) variant point mutation (E1021A).DepositorInsertVcnTS-E1021A (VCL Chicken, Synthetic)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1021 to alanine (E…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-S1033A
Plasmid#211835PurposeVinculin tension sensor (VinTS) with vinculin S1033 unphosphorylatable point mutation (S1033A), in lentiviral expression vector.DepositorInsertVinculinTS-S1033A (VCL Chicken, Synthetic)
UseLentiviralTagsExpressionMutationmutated vinculin serine 1033 to alanine (S1033A),…PromoterCMVAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VinculinTS-S1033D
Plasmid#211893PurposeVinculin tension sensor (VinTS) with vinculin S1033 phosphomimetic point mutation (S1033D), in lentiviral expression vector.DepositorInsertVinculinTS-S1033D (VCL Chicken, Synthetic)
UseLentiviralTagsExpressionMutationmutated vinculin serine 1033 to aspartic acid (S1…PromoterCMVAvailable sinceJan. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgINTS12 guide 2
Plasmid#193607PurposeINTS12 knockoutDepositorInsertsgINTS12 guide 2 (INTS12 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgINTS12 guide 1
Plasmid#193606PurposeINTS12 knockoutDepositorInsertsgINTS12 guide 1 (INTS12 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLV-MNTS-1-p75
Plasmid#182945PurposeTo express a multi-neurotrophin made from the Human NT-3 gene. The secreted protein that can bind only all 3 TRK receptors and not p75NTRDepositorUseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV promoter and enhancerAvailable sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 NtSPrbcS (GB2213)
Plasmid#160589PurposeB3 for signal peptide (transit peptide to chloroplast) from Ribulose bisphosphate carboxylase small chainDepositorInsertNtSPrbcS
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSUI-SynP-CANTsg
Plasmid#154343PurposeExpression of the sgRNA targeting to the E. coli can geneDepositorInsertCANT sgRNA
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTOPO-rDNA-NTS
Plasmid#23992DepositorInsertrDNA NTS
UseBacterial cloning - topoTagsExpressionMutationNonePromoterAvailable sinceAug. 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-DIO-GRAB_NTS1.0
Plasmid#208671PurposeExpresses the genetically-encoded fluorescent neurotensin (NTS) sensor GRAB_NTS1.0 in a cre-dependent mannerDepositorInsertGPCR activation based neurotensin (NTS) sensor GRAB_NTS1.0
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6L
Plasmid#198409PurposeExpresses FLAG-tagged human IntS6LDepositorInsertIntegrator complex subunit 6 like (INTS6L Human)
UseTags3x FLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6
Plasmid#198408PurposeExpresses FLAG-tagged human IntS6DepositorInsertDDX26 (INTS6 Human)
UseTags3x FLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS6 AA 1035-1284
Plasmid#196904PurposeExpresses His-tagged Drosophila IntS6 amino acids 1035-1284DepositorInsertIntegrator subunit 6 (IntS6 Fly)
UseTags6xHisExpressionBacterialMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1 IntS11 AA 300-597
Plasmid#199329PurposeExpresses His-tagged Drosophila IntS11 amino acids 300-597DepositorInsertIntegrator subunit 11 (IntS11 Fly)
UseTags6xHisExpressionBacterialMutationamino acids 300-597 onlyPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
8xLexA-binding elements/pMD20
Plasmid#48807PurposeContains 8xLexA-binding elementsDepositorInsert8xLexA-binding elements
UseTagsExpressionMutationPromoterAvailable sinceDec. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-VcnTS-E1015A-E1021A
Plasmid#213417PurposeVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A).DepositorInsertVcnTS-E1015A-E1021A (VCL Chicken, Synthetic)
UseTagsExpressionMammalianMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRRL-VcnTS-E1015A-E1021A
Plasmid#213411PurposeVinculin tension sensor (VcnTS) with both directionally asymmetric, force strengthening (DAFS) variant point mutations (E1015A and E1021A), in lentiviral expression vector.DepositorInsertVcnTS-E1015A-E1021A (VCL Chicken, Synthetic)
UseLentiviralTagsExpressionMutationMutated vinculin glutamic acid 1015 and 1021 to a…PromoterCMVAvailable sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only