We narrowed to 593 results for: en1

1. pBEN1-SGC

   Plasmid

   #39127

   Purpose

   SGC Empty backbone for bacterial expression

   Depositor

   Nicola Burgess-Brown

   Type

   Empty backbone

   Use

   Tags

   Strep tag and SET1

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

2. pAFMW-AthHEN1

   Plasmid

   #104980

   Purpose

   Expression of FLAG Myc tagged A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

   Depositor

   Stefan Ameres

   Article

   Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

   Insert

   Hen1 (HEN1 Mustard Weed)
   

   Use

   Tags

   FLAG Myc

   Expression

   Insect

   Mutation

   Promoter

   Actin5C

   Available since

   Jan. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEN12A-P750

   Plasmid

   #49525

   Purpose

   Ampicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sites

   Depositor

   Dirk Schnappinger

   Article

   Kim et al Proc Natl Acad Sci U S A. 2013 Nov 4.

   Insert

   P750
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   P750

   Available since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. BirA-Myc_N_HEN1

   Plasmid

   #167708

   Purpose

   Expresses HEN1 in mammalian cells

   Depositor

   Markku Varjosalo

   Article

   Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

   Insert

   HEN1 (NHLH1 Human)
   

   Use

   Tags

   Myc-BirA

   Expression

   Mammalian

   Mutation

   no mutations

   Promoter

   Available since

   April 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRSETb-rsGreen1

   Plasmid

   #78180

   Purpose

   Expresses rsGreen1 in E. coli strains.

   Depositor

   Peter Dedecker

   Article

   Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

   Insert

   rsGreen1
   

   Use

   Tags

   6xHis-tag; Xpress tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pShuttle-FEN1mED

   Plasmid

   #35033

   Depositor

   Sheila Stewart

   Article

   Saharia et al J Biol Chem. 2010 Aug 27;285(35):27057-6

   Insert

   FEN1mWT
   

   Use

   Adenoviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   PT MUTANT E160D

   Promoter

   CMV

   Available since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

7. ZsGreen1-CytERM-N-17

   Plasmid

   #56297

   Purpose

   Localization: Endoplasmic Reticulum, Excitation: 493, Emission: 505

   Depositor

   Michael Davidson

   Insert

   CytERM
   

   Use

   Tags

   ZsGreen1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCMV-MARWOLEN1

   Plasmid

   #217225

   Purpose

   Mammalian expression vector for expression of MARWOLEN1 transposase (CMV promoter)

   Depositor

   Yong E Zhang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   MARWOLEN1 transposase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMV-MARWOLEN1

   Plasmid

   #217285

   Purpose

   Donor plasmid of MARWOLEN1 Transposon

   Depositor

   Yong E Zhang

   Article

   Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

   Insert

   TIRs of MARWOLEN1 transposon
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAFMW-AthHEN1-CM

    Plasmid

    #104981

    Purpose

    Expression of FLAG Myc tagged catalytic dead A.thaliana-Hen1 (codon optimised for Drosophila) in Drosophila S2 cells

    Depositor

    Stefan Ameres

    Article

    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461

    Insert

    Hen1 (HEN1 Mustard Weed)
    

    Use

    Tags

    FLAG Myc

    Expression

    Insect

    Mutation

    Promoter

    Actin5C

    Available since

    Jan. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-EF1alpha-ZsGreen1_Puro

    Plasmid

    #192661

    Purpose

    3rd generation lenti vector encoding ZsGreen1 with 2A Puro resistance marker

    Depositor

    Jeffrey Miner

    Insert

    ZsGreen1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti_sgRNA(MS2)_zsGreen1

    Plasmid

    #138463

    Purpose

    lenti sgRNA cloning backbone with MS2 loops and EF1a-zsGreen1

    Depositor

    Jian Xu

    Article

    Li et al Nat Commun. 2020 Jan 24;11(1):485. doi:

    Insert

    zsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    May 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PX458-3xHA-en1FnCas9

    Plasmid

    #201947

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en1FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1369R on FnCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-EF1alpha-ZsGreen1_Blast

    Plasmid

    #192660

    Purpose

    3rd generation lenti vector encoding ZsGreen1 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Insert

    ZsGreen1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBABE hygro MEN1 WT

    Plasmid

    #11024

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pLVX_BHLHE40-FlagHA_IRES-ZsGreen1

    Plasmid

    #149714

    Purpose

    Lentiviral vector expressing human BHLHE40 with FLAG-HA tags

    Depositor

    Silvia Monticelli

    Article

    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi

    Insert

    BHLHE40 (BHLHE40 Human)
    

    Use

    Lentiviral

    Tags

    FLAG-HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1α

    Available since

    Sept. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PX458-3xHA-en15FnCas9

    Plasmid

    #201948

    Purpose

    Mammalian expression plasmid of enhanced activity FnCas9 variant en15FnCas9 with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    3xHA-NLS-en15FnCas9-T2A-EGFP
    

    Use

    CRISPR

    Tags

    3xHA, NLS, and T2A-EGFP

    Expression

    Mammalian

    Mutation

    E1603H on FnCas9

    Promoter

    Cbh

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FEN1 C10.3 gRNA

    Plasmid

    #90689

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FEN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FEN1 (Guide Designation C10.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pTH-Cen15-mVenus

    Plasmid

    #49642

    Purpose

    TALEColor-mVenus-Chromosome 15-specific Centromere

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

    Insert

    TALEColor-Cen15
    

    Use

    TALEN

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTH-Cen18-mVenus

    Plasmid

    #49641

    Purpose

    TALEColor-mVenus-Chromosome 18-specific Centromere

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

    Insert

    TALEColor-Cen18
    

    Use

    TALEN

    Tags

    mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. FEN1 C9.3 gRNA

    Plasmid

    #90688

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human FEN1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    FEN1 (Guide Designation C9.3)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. USPQ-HTT-TALEN1

    Plasmid

    #92243

    Purpose

    TALEN targeting upstream of HTT gene (KKR), forms obligate heterodimer with USPQ-HTT-TALEN2

    Depositor

    Mahmoud Pouladi

    Article

    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

    Insert

    TALEN
    

    Use

    TALEN

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET-His6-en1dFnCas9GFP

    Plasmid

    #201950

    Purpose

    Expression of en1dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en1dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1369R on FnCas9

    Promoter

    T7

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET-His6-en15dFnCas9GFP

    Plasmid

    #201951

    Purpose

    Expression of en15dFnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Nat Commun. 2024 Jun 28;15(1):5471. doi:

    Insert

    Expression of en15dFnCas9-GFP in bacterial cells
    

    Use

    CRISPR

    Tags

    6x His, HA, NLS, and mEGFP

    Expression

    Bacterial

    Mutation

    E1603H on FnCas9

    Promoter

    T7

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTRE3G-ZSGreen1-mHP1alpha

    Plasmid

    #181913

    Purpose

    Inducible mammalian expression vector for ZsGreen1-tagged HP1alpha

    Depositor

    Karsten Rippe

    Article

    Erdel et al Mol Cell. 2020 Apr 16;78(2):236-249.e7.

    Insert

    mHP1alpha (Cbx5 Mouse)
    

    Use

    Tags

    ZSGreen1

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pX330-EN1680_Sororin-Cterm

    Plasmid

    #156451

    Purpose

    For transient expression of Cas9 and sgRNA targeting mouse Cdc5a (SORORIN) stop codon

    Depositor

    Elphege Nora

    Article

    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

    Insert

    Cas9, SORORIN (Cdca5 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAAVS1-NDi-CRISPRi (Gen1)

    Plasmid

    #73497

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus with mCherry marker

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB-P2A-mCherry
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. ZsGreen1-cMYC/pLVX-Puromycin

    Plasmid

    #180278

    Purpose

    Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    ZsGreen1-cMYC fusion protein MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. RNA motif plasmid EDEN15

    Plasmid

    #107252

    Purpose

    EDEN15 RNA motif

    Depositor

    Paul Khavari

    Article

    Ramanathan et al Nat Methods. 2018 Feb 5. pii: nmeth.4601

    Insert

    EDEN15 motif
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBABE hygro MEN1 A242V

    Plasmid

    #11022

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MEN1 A242V (MEN1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    A242V

    Promoter

    Available since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only