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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 1,093 results for: rbis

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Showing: 31 - 60 of 1093 results

  3. OfCasp3b

    Plasmid
    #166054
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but function similar to caspase-6
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3b
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166054-plasmid-map-sequence-id-328273

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  4. OfCasp3a

    Plasmid
    #166053
    Purpose
    a caspase of Orbicella faveolata, which has higher sequence similarity with human caspase-3 but has CARD domain and function as an initiator caspase
    Depositor
    Clay Clark
    Article
    Shrestha et al J Biol Chem. 2020 Oct 23;295(43):14578-1
    Insert
    Orbicella faveolata caspase-3a
    Tags
    His tag
    Expression
    Bacterial
    Promoter
    T7 lac promoter
    Available Since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166053-plasmid-map-sequence-id-328272

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  5. pB1H2 UV5 Zif268-cons (2-hybrid bait plasmid)

    Plasmid
    #128164
    Purpose
    UV5 promoter drives expression of Zif268-consensus peptide fusion. Pair with pGHUC w-ERBIN as a positive control (turns on HIS3/GFP).
    Depositor
    Marcus Noyes
    Article
    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928
    Insert
    10 amino acid glycine/serine linker + ERBIN PDZ consensus ligand (WETWV)
    Use
    Synthetic Biology
    Tags
    FLAG and zif268
    Expression
    Bacterial
    Promoter
    UV5
    Available Since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    128164-plasmid-map-sequence-id-267570

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  6. pUMN105

    Plasmid
    #233024
    Purpose
    Encodes eGFP for ORBIT integration. High copy number.
    Depositor
    Anthony Baughn
    Type
    Empty backbone
    Available Since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    233024-plasmid-map-sequence-id-467921

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  13. pInt_attP1_sacB_kanR

    Plasmid
    #205301
    Purpose
    Integrating plasmid for ORBIT in E. coli with sacB for counter selection. Used for markerless or scarless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205301-plasmid-map-sequence-id-404791

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  14. pInt_attP1_LCS_kanR

    Plasmid
    #205300
    Purpose
    Integrating plasmid for ORBIT in E. coli. Contains library cloning site (LCS), which has transcriptional terminators to insulate reporter constructs.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Sept. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    205300-plasmid-map-sequence-id-407004

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  15. pInt_attP1_tsXis_sacB_kanR

    Plasmid
    #205302
    Purpose
    Integrating plasmid for ORBIT in E. coli with self excising factor (Xis) and sacB for counter selection. Used for markerless modifications.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Type
    Empty backbone
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205302-plasmid-map-sequence-id-404790

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  16. pHelper_NoMutL_V2_gentR

    Plasmid
    #205293
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Available Since
    Oct. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    205293-plasmid-map-sequence-id-410127

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  17. pHelper_Ec1_V1_gentR

    Plasmid
    #205291
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Available Since
    Aug. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205291-plasmid-map-sequence-id-405409

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  18. pHelper_Ec1_V1_ampR

    Plasmid
    #205292
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Has sacB for counter selection.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    205292-plasmid-map-sequence-id-404792

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  19. pHelper_TS_V2_ampR

    Plasmid
    #214467
    Purpose
    Helper plasmid for ORBIT in E. coli. Expresses RecT for oligo recombineering and Bxb-1 for pInt incorporation. Includes temperature sensitive origin for plasmid curing.
    Depositor
    Scott Saunders
    Article
    Saunders et al bioRxiv 2023
    Insert
    CspRecT / Bxb-1
    Use
    Synthetic Biology
    Promoter
    XylS / AraC
    Available Since
    April 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    214467-plasmid-map-sequence-id-429338

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Showing: 31 - 60 of 1093 results