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Showing: 41 - 80 of 25998 results

  1. pCAT100

    Plasmid
    #180512
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180512-plasmid-map-sequence-id-359730

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  2. pCAT105

    Plasmid
    #180510
    Purpose
    Plasmid expressing mammalian codon optimized engineered DpbCasX-R3, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    DpbCasX with R3 loop
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180510-plasmid-map-sequence-id-359734

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  3. pCAT527

    Plasmid
    #180514
    Purpose
    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180514-plasmid-map-sequence-id-359727

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  6. Bcatfl

    Plasmid
    #24195
    Depositor
    David Rimm
    Insert
    beta-catenin (CTNNB1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 9, 2010
    Availability
    Academic Institutions and Nonprofits only

  7. pCAT024

    Plasmid
    #171636
    Purpose
    Plasmid containing a U6 promoter expressing a spyCas9 sgRNA targeting BFP and CAG promoter expressing mTagBFP2
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Inserts
    spyCas9 sgRNA-BFP
    mTagBFP2
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    July 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    171636-plasmid-map-sequence-id-337539

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  20. pET:GCAT:GFP

    Plasmid
    #60736
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60736-plasmid-map-sequence-id-97722

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  24. pET:ACAT:GFP

    Plasmid
    #60740
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60740-plasmid-map-sequence-id-97725

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  25. pCAT526

    Plasmid
    #180513
    Purpose
    Plasmid expressing mammalian codon optimized wt PlmCasX, mNeonGreen, sgRNAv1 scaffold, and restriction sites to clone in new spacers
    Depositor
    Jennifer Doudna
    Article
    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6
    Inserts
    CasX sgRNAv1
    PlmCasX-2A-mNeonGreen
    Use
    CRISPR
    Tags
    2x FLAG and SV40 NLS
    Expression
    Mammalian
    Mutation
    Promoter
    CAG and U6
    Available since
    April 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    180513-plasmid-map-sequence-id-359728

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  37. pETM6_Ptrc_BsMdh_BmHps_BmPhi_Pthl_CaThl_CaCtfAB_CaAdc

    Plasmid
    #129105
    Purpose
    Expresses Mdh from Bacillus subtilis and Hps & Phi from Bacillus methanolicus as well as Clostridium acetobutylicum genes required for Acetate-dependent Acetone production
    Depositor
    Eleftherios Terry Papoutsakis
    Article
    Bennett et al Metab Eng. 2018 Jan;45:75-85. doi: 10.10
    Insert
    BsMDH BmHPS BmPHI CaTHL CaCtfAB CaADC
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 11, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    129105-plasmid-map-sequence-id-255085

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  38. pTUBmCardinalSagCAT.dna

    Plasmid
    #166236
    Purpose
    mCardinal consituitive reporter, CAT marker
    Depositor
    David Sibley
    Insert
    mCardinal
    Use
    Tags
    Expression
    Mutation
    none
    Promoter
    Available since
    April 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    166236-plasmid-map-sequence-id-329836

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  39. MCAT

    Plasmid
    #38858
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MCAT (MCAT Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 41 - 80 of 25998 results