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Showing: 41 - 80 of 2899 results

  3. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

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  5. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid
    #229677
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    SNAP, 3xV5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229677-plasmid-map-sequence-id-457129

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  8. FLAG-LoxP-PURO-LoxP-HaloTERT WT HR Donor

    Plasmid
    #86843
    Purpose
    HR Donor for N-terminal FLAG-HaloTag-TERT fusion in human cells. Includes Puro selection marker flanked by LoxP sites.
    Depositor
    Thomas Cech
    Article
    Schmidt et al Cell. 2016 Aug 25;166(5):1188-1197.e9. d
    Insert
    FLAG-HaloTag-TERT Exon 1 and part of Exon 2 (TERT Human)
    Use
    Tags
    FLAG-HaloTag
    Expression
    Mammalian
    Mutation
    Promoter
    Endogenous TERT promoter
    Available since
    March 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    86843-plasmid-map-sequence-id-174395

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  9. FLAG-LoxP-PURO-LoxP-SNAP-TERT

    Plasmid
    #71390
    Purpose
    Homologues recombination donor plasmid for inserting a FLAG-SNAP-tag at the N-terminus of the TERT locus in human cells. Includes PURO resistance flanked by LoxP sites for selection.
    Depositor
    Thomas Cech
    Article
    Xi et al Genome Biol. 2015 Nov 10;16(1):231. doi:
    Insert
    TERT (TERT Human)
    Use
    Hr donor
    Tags
    FLAG-SNAP
    Expression
    Mutation
    Promoter
    Endogenous TERT promotor
    Available since
    Dec. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    71390-plasmid-map-sequence-id-128451

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  13. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid
    #198240
    Purpose
    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites
    Depositor
    Didier Stainier
    Article
    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.
    Inserts
    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 3, 2024
    Availability
    Academic Institutions and Nonprofits only
    198240-plasmid-map-sequence-id-393627

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  14. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

    Plasmid
    #195503
    Purpose
    SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
    Use
    CRISPR; Donor vector
    Tags
    T2A-H2B-mCitrine
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195503-plasmid-map-sequence-id-384989

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  29. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid
    #227246
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227246-plasmid-map-sequence-id-450569

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  32. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid
    #227245
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227245-plasmid-map-sequence-id-450568

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Showing: 41 - 80 of 2899 results