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Showing: 51 - 100 of 848 results

  7. cEF1a-GFP

    Plasmid
    #135671
    Purpose
    Introduce constitutive (EF1a promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    135671-plasmid-map-sequence-id-325845

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  8. cSFFV-GFP

    Plasmid
    #135670
    Purpose
    Introduce constitutive (SFFV promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    135670-plasmid-map-sequence-id-325843

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  9. AICSDP-63: ACTN2-mEGFP

    Plasmid
    #124607
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human ACTN2, via excisable Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    ACTN2 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (ACTN2 Human)
    Use
    CRISPR; Donor template
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 13, 2019
    Availability
    Academic Institutions and Nonprofits only
    124607-plasmid-map-sequence-id-244336

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  12. cTRE-GFP

    Plasmid
    #135665
    Purpose
    Introduce Dox-inducible (TRE promoter) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135665-plasmid-map-sequence-id-325434

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  13. pmEGFP-1

    Plasmid
    #36409
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Use
    Fluorescent protein cassette for sub-cloning
    Tags
    mEGFP
    Expression
    Mutation
    Promoter
    no promoter
    Available since
    May 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    36409-plasmid-map-sequence-id-45554

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  14. pmEGFP-13

    Plasmid
    #36410
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Use
    Fluorescent protein cassette for sub-cloning
    Tags
    3XmEGFP
    Expression
    Mutation
    Promoter
    No promoter
    Available since
    May 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    36410-plasmid-map-sequence-id-45555

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  15. pmEGFP-16

    Plasmid
    #36411
    Depositor
    Benjamin Glick
    Type
    Empty backbone
    Use
    Fluorescent protein cassette for sub-cloning
    Tags
    6X mEGFP
    Expression
    Mutation
    Promoter
    No promoter
    Available since
    May 15, 2012
    Availability
    Academic Institutions and Nonprofits only
    36411-plasmid-map-sequence-id-45557

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  16. C-SWAT GFP CL1 DAmP

    Plasmid
    #182513
    Purpose
    C-SWAT compatible vector for C-terminal tagging with GFP CL1 followed by the DAmP cassette. The stop codon after the CL1 degron is immediately followed by the Hygromycin B (Hph) resistance cassette.
    Depositor
    Rob Mitra
    Article
    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102
    Insert
    CL1 and DAmP
    Use
    Tags
    GFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    182513-plasmid-map-sequence-id-358273

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  19. AICSDP-55:DMD-mEGFP

    Plasmid
    #159744
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human DMD via Cas9-excisable CAGGS-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Insert
    DMD Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (DMD Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 13, 2020
    Availability
    Academic Institutions and Nonprofits only
    159744-plasmid-map-sequence-id-314374

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  20. pT2-HSP-GFP

    Plasmid
    #65488
    Purpose
    transposable GFP
    Depositor
    Guillaume Filion
    Insert
    GFP transposable cassette (amaxaGFP)
    Use
    Tags
    Expression
    Mutation
    Promoter
    hsp70
    Available since
    Dec. 19, 2017
    Availability
    Academic Institutions and Nonprofits only
    65488-plasmid-map-sequence-id-117818

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  21. pRA1SEGFPTcIfm

    Plasmid
    #193777
    Purpose
    Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
    Depositor
    Sangram Bagh
    Article
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PR promoter and PLTetO-1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193777-plasmid-map-sequence-id-389829

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  31. R6K_tac_GFPmut3_ChInt

    Plasmid
    #187381
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible GFPmut3
    Depositor
    Roi Avraham
    Insert
    GFPmut3
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187381-plasmid-map-sequence-id-370413

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  33. pAAV-flex-GFP-shRNA-scramble

    Plasmid
    #182503
    Purpose
    Expresses scramble shRNA for control expts. Flexed cassette driven by pan neuronal gene promoter
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal promoter driving flexed GFP-shRNA scramble from mir 30 cassette
    Use
    AAV, Cre/Lox, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hSyn promoter
    Available since
    March 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    182503-plasmid-map-sequence-id-355964

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  35. BS II KS+ loxP-GFP_ZEO_loxP

    Plasmid
    #128064
    Purpose
    has loxp-GFP-Zeo-Loxp cassette
    Depositor
    Oskar Laur
    Insert
    loxp-GFP-Zeo-Loxp
    Use
    Cre/Lox
    Tags
    Expression
    Mutation
    no
    Promoter
    Available since
    Nov. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    128064-plasmid-map-sequence-id-255173

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  36. pAS_4xMmaPylT_EF1_mCherry_TAG_EGFP

    Plasmid
    #174893
    Purpose
    amber suppression reporter mCherry-TAG-EGFP expression, with MmaPylT amber suppressor tRNA cassette
    Depositor
    Simon Elsaesser
    Insert
    mCherry and EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1
    Available since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    174893-plasmid-map-sequence-id-344146

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  37. R6K_BAD_GFP-mCherry_ChInt++

    Plasmid
    #187393
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherry
    Depositor
    Roi Avraham
    Insert
    GFP, mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187393-plasmid-map-sequence-id-371128

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  38. R6K_BAD_GFP-mCherry_ChInt--

    Plasmid
    #187394
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherry
    Depositor
    Roi Avraham
    Insert
    GFP, mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187394-plasmid-map-sequence-id-371125

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  39. R6K_BAD_mCherry-GFP_ChInt--

    Plasmid
    #187396
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette encoding for arabinose-inducible GFP and mCherry
    Depositor
    Roi Avraham
    Insert
    GFP, mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187396-plasmid-map-sequence-id-371114

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  40. p1.2-GS-eGFP

    Plasmid
    #162771
    Purpose
    Fluorescent reporter for CHO expression studies
    Depositor
    Ivan Vorobiev
    Article
    Kovnir et al Bull Exp Biol Med. 2017 Jun;163(2):245-2
    Insert
    enhanced green fluorescent protein
    Use
    Tags
    Expression
    Mammalian
    Mutation
    consensus Kozak sequence (GCCGCCATGG) added befor…
    Promoter
    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…
    Available since
    Aug. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    162771-plasmid-map-sequence-id-339094

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  41. pAcBac2.tR4-OMeYRS/GFP*

    Plasmid
    #50831
    Purpose
    baculovirus-based delivery system that enables the efficient incorporation of unnatural amino acids into proteins in mammalian cells
    Depositor
    Peter Schultz
    Article
    Chatterjee et al Proc Natl Acad Sci U S A. 2013 Jul 16;11
    Inserts
    two-copy Tyr tRNA cassette
    EGFP*
    OMeYRS
    two copy Tyr tRNA cassette
    Use
    Baculoviral
    Tags
    His, Myc-6xHis, and WPRE
    Expression
    Insect
    Mutation
    Y39TAG and able to charge various unnatural amino…
    Promoter
    CAG, CMV, and U6 and H1
    Available since
    June 3, 2015
    Availability
    Academic Institutions and Nonprofits only
    50831-plasmid-map-sequence-id-193569

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  45. cEF1a-LSL-GFP

    Plasmid
    #135672
    Purpose
    Introduce Cre-inducible (EF1a promoter followed by lox-stop-lox) GFP cDNA into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    GFP
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135672-plasmid-map-sequence-id-325851

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  46. cTRE-GFP-miR30_shRen

    Plasmid
    #135666
    Purpose
    Introduce Dox-inducible (TRE promoter) GFP cDNA plus miR30-embedded shRenilla into (ES) cells by recombination-mediated cassette exchange
    Depositor
    Florian Karreth
    Insert
    shRenilla
    Use
    Mouse Targeting
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    135666-plasmid-map-sequence-id-325436

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  47. INT_Cargo_PgyrA_gfp

    Plasmid
    #212138
    Purpose
    Cargo plasmid for integrating PgyrA expression cassette of green fluorescent protein in genome.Plasmid can be removed by incubating cells at 37 C.
    Depositor
    Kang Zhou
    Insert
    green fluorescent protein
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PgyrA
    Available since
    Jan. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    212138-plasmid-map-sequence-id-420768

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  48. R6K_tac_dGFPmut3_ChInt

    Plasmid
    #187388
    Purpose
    chromosomal integration downstream of glmS gene via Tn7 system of a cassette containing IPTG-inducible destabilized GFPmut3 (containing a AAV C-terminal tail)
    Depositor
    Roi Avraham
    Insert
    dGFPmut3_AAV
    Use
    Tags
    AAV
    Expression
    Bacterial
    Mutation
    C-terminal AAV tail
    Promoter
    Available since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    187388-plasmid-map-sequence-id-370402

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  49. AICSDP-34: TNNI1-mEGFP

    Plasmid
    #114411
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human TNNI1, via excisable Cas9-excisable hPGK-mCherry selection cassette
    Depositor
    Allen Institute for Cell Science
    Article
    Roberts et al Stem Cell Reports. 2019 May 14;12(5):114
    Insert
    TNNI1 Homology Arms with linker-mEGFP and Cas9-excisable selection cassette (TNNI1 Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114411-plasmid-map-sequence-id-220877

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  50. pAAV-flex-GFP-shRNA-GluN1

    Plasmid
    #182502
    Purpose
    Expresses shRNA against mouse NMDA receptor NR1 subunit. Flexed cassette driven by hdc (pan neuronal) gene promoter
    Depositor
    William Wisden
    Article
    Miracca et al J Neurosci. 2022 May 31. pii: JNEUROSCI.
    Insert
    pan neuronal promoter (hdc basal promoter) driving flexed-GFP-mir30-shRNA NR1 cassette
    Use
    AAV, Cre/Lox, and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    fragment of histidine decarboxylase gene promoter…
    Available since
    March 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    182502-plasmid-map-sequence-id-355962

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Showing: 51 - 100 of 848 results