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Showing: 21 - 40 of 12598 results

  1. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Gregg Beckham, Christopher Johnson
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112203-plasmid-map-sequence-id-473446

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  3. pNR2F1.1.0-gDNA

    Plasmid
    #112484
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR2F1
    Depositor
    Kevin White
    Insert
    NR2F1 (NR2F1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112484-plasmid-map-sequence-id-230521

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  7. pCINEO-3xFlag-hRIPK3

    Plasmid
    #112495
    Purpose
    Mammalian expression of 3xFlag-hRIPK3
    Depositor
    Junying Yuan
    Insert
    RIPK3 (RIPK3 Human)
    Tags
    Flag
    Expression
    Mammalian
    Available Since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    112495-plasmid-map-sequence-id-214535

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  8. pESR1.1.0-gDNA

    Plasmid
    #112433
    Purpose
    CRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor ESR1
    Depositor
    Kevin White
    Insert
    ESR1 (ESR1 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Dec. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    112433-plasmid-map-sequence-id-230374

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  10. pCFD5_w

    Plasmid
    #112645
    Purpose
    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.
    Depositor
    Michael Boutros
    Insert
    U6:3-tRNA-sgRNA expression cassette
    Expression
    Insect
    Available Since
    Aug. 13, 2018
    Availability
    Academic Institutions and Nonprofits only
    112645-plasmid-map-sequence-id-217990

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  11. pESR1-donor

    Plasmid
    #112337
    Purpose
    CRISPR donor plasmid to tag human transcription factor ESR1 with GFP
    Depositor
    Kevin White
    Insert
    ESR1 homology arms flanking EGFP-IRES-Neo cassette (ESR1 Human)
    Use
    CRISPR
    Mutation
    Unknown
    Available Since
    April 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    112337-plasmid-map-sequence-id-241244

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  14. lentiCRISPR v2-dCas9

    Plasmid
    #112233
    Purpose
    Lentiviral plasmid for expression of gRNA and dCas9 in mammalian cells; derivative of lentiCRISPR v2
    Depositor
    Thomas Gilmore
    Article
    Babaei et al PLoS One. 2019 Sep 25;14(9):e0222588. do
    Inserts
    dCas9
    Porumycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Changed: Aspartic acid 10 to Glycine, Histidine 8…
    Promoter
    EFS-NS
    Available Since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    112233-plasmid-map-sequence-id-213540

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  16. pGDP3 rmtB

    Plasmid
    #112880
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    rmtB (RmtB Escherichia coli)
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    Pbla
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112880-plasmid-map-sequence-id-219870

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  17. pGDP3 aph(3'')-Ia

    Plasmid
    #112879
    Purpose
    This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.
    Depositor
    Gerard Wright
    Article
    Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109
    Insert
    aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)
    Tags
    6xHis
    Expression
    Bacterial
    Promoter
    Pbla
    Available Since
    Dec. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112879-plasmid-map-sequence-id-219874

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  19. pTU2-AmyE-Pveg-eGFP

    Plasmid
    #112792
    Purpose
    Expresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformation
    Depositor
    Chris French
    Article
    Wicke et al bioRxiv 185108
    Insert
    Pveg-eGFP
    Expression
    Bacterial
    Available Since
    Aug. 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    112792-plasmid-map-sequence-id-218394

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  20. pNR4A1-donor

    Plasmid
    #112295
    Purpose
    CRISPR donor plasmid to tag human transcription factor NR4A1 with GFP
    Depositor
    Kevin White
    Insert
    NR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
    Use
    CRISPR
    Mutation
    hg38:12:52058840:C>T, hg38:12:52058930:A>C,…
    Available Since
    Nov. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    112295-plasmid-map-sequence-id-228416

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Showing: 21 - 40 of 12598 results