-
Plasmid#112894Purposepromoter-inducible expression of constitutively-active RhoADepositorInsertCA RhoA (RHOA Human)
UseLentiviralTagsExpressionBacterialMutationPromoterAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLL3.7-EF-EYFP-YAP1_WT-PolyA
Plasmid#112284PurposeFluorescently labels WT human YAP1-2gamma isoform (504 aa)DepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralTagsExpressionMutationWT human YAP1-2gammaPromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTU2-AmyE-Pveg-eGFP
Plasmid#112792PurposeExpresses GFP under the control of Pveg promoter and it is integrated into AmyE locus. Used to test correct transformationDepositorArticleInsertPveg-eGFP
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBP-Pspac
Plasmid#112778PurposePromoter repressed by LacI (not natively produced in B. subtilis 168)DepositorArticleInsertPspac promoter
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/rh10
Plasmid#112866PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap-rh10
UseAAVTagsExpressionMutationPromoterTruncated P5Available sinceSept. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET21b(+)-Is-PETase
Plasmid#112202PurposepET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1), codon optimized for expression in E. coli K12DepositorInsertPETase gene from Ideonella sakaiensis 201-F6, codon optimized for expression in E. coli K12
UseTags6XHISExpressionBacterialMutationPromoterN/AAvailable sinceJuly 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMM627
Plasmid#112532PurposepZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanRDepositorInsertsHrtR
ChuA
luxCDABE
UseTagsExpressionBacterialMutationPromoterJ23107, PL(HrtO), and ProDAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMM534
Plasmid#112530PurposepZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanRDepositorInsertluxCDABE
UseTagsExpressionBacterialMutationPromoterPL(HrtO)Available sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/1
Plasmid#112862PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap1
UseAAVTagsExpressionMutationPromoterTruncated P5Available sinceAug. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV2/7
Plasmid#112863PurposeAAV packaging plasmid expressing Rep/Cap genesDepositorInsertRep2/Cap7
UseAAVTagsExpressionMutationPromoterTruncated P5Available sinceSept. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLBH531_MBP-Cas14a1 expression
Plasmid#112500PurposeExpresses MBP fused to Cas14a1DepositorInsertCas14a1
UseCRISPRTags10xHis-MBPExpressionBacterialMutationPromoterT7Available sinceOct. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAGG-NFIX1
Plasmid#112702PurposeExpression of NFIX1 protein and GFPDepositorInsertNuclear Factor I X1 (Nfix Mouse)
UseTagsGFP and HAExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ubiq-Cas9.874W
Plasmid#112686PurposeExpress hSpCas9 under Ubiquitin-63E promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoterUbiquitin-63E promoterAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
nos-Cas9.874Z1
Plasmid#112685PurposeExpress hSpCas9 under nanos promoterDepositorInserthSpCas9
UseTags3x FLAG, EGFP, and NLSExpressionInsectMutationHumanized Cas9 bacterial sequencePromoternanosAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
Ga13_2-mTq2
Plasmid#112933PurposeHuman Galpha13 tagged with mTurquoise, inserted at residue 128DepositorInsertGalpha13
UseTagsmTurquoise2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
G13 sensor (Ga13_2)
Plasmid#112930PurposeA FRET sensor for G13 activation, encoded on a single plasmid.DepositorInsertG13 sensor
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLJM1 Raptor GFP
Plasmid#112744Purposeexpress Raptor-GFPDepositorInsertRaptor-GFP (RPTOR Human)
UseLentiviralTagseGFPExpressionMutationPromoterAvailable sinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-RNMT
Plasmid#112705PurposeExpresses N-terminally FLAG-tagged human RNMT in mammalian cellsDepositorInsertRNMT (RNMT Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceMarch 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSynapsin1-axon-GCaMP6s-P2A-mKate2
Plasmid#112004PurposeFor enriched expression of GCaMP6s in axons with cytosolic expression of mKate2DepositorHas ServiceAAV9Insertaxon-GCaMP6s-P2A-mKate2
UseAAVTagsGAP43 palmitoylation domainExpressionMammalianMutationPromoterhSynapsin1Available sinceJune 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEv-Fat4-citrine
Plasmid#112854PurposeConstitutive expression of human fat4 protein fused to citrine fluorescent proteinDepositorInsertFAT4 (FAT4 Human)
UseTagsCitrineExpressionMammalianMutationPromoterCMVAvailable sinceMay 28, 2019AvailabilityAcademic Institutions and Nonprofits only