Skip to main content
Addgene
Showing: 21 - 40 of 188 results

  1. pAAV-EF1a-hfCas13d-pa

    Plasmid
    #233037
    Purpose
    To express hfCas13d from a EF1a promoter
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233037-plasmid-map-sequence-id-463798

    Enlarge

  2. pUt-mNF-Cas13d

    Plasmid
    #224789
    Purpose
    LPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Use BlastR for positive selection and HSV-TK for negative selection.
    Depositor
    Gabor Balazsi
    Article
    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-
    Insert
    mCherry-P2A-RfxCas13d
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV-d2i
    Available since
    Oct. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    224789-plasmid-map-sequence-id-445954

    Enlarge

  4. lenti-RfxCas13d-Blast-BFP

    Plasmid
    #196726
    Purpose
    Sortable and selectable RfxCas13d.
    Depositor
    Bernhard Schmierer
    Insert
    RfxCas13d-T2A-BSD-TagBFP
    Use
    CRISPR and Lentiviral
    Tags
    SV40 NLS
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    196726-plasmid-map-sequence-id-389737

    Enlarge

  8. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

    Plasmid
    #233035
    Purpose
    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter
    Depositor
    Jonathan Ploski
    Insert
    RfxCas13d-compatible gRNA expression cassette and CasRx
    Use
    AAV
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    233035-plasmid-map-sequence-id-467423

    Enlarge

  9. pAAV-EFS-hfCas13d-HA-mCherry-pA

    Plasmid
    #233029
    Purpose
    To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233029-plasmid-map-sequence-id-464625

    Enlarge

  10. pAAV-EFS-DjCas13d-HA-mCherry-pA

    Plasmid
    #233030
    Purpose
    To Express HA tagged DjCas13d-mCherry fusion protein from the mammalian EFS promoter
    Depositor
    Jonathan Ploski
    Insert
    DjCas13d-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    233030-plasmid-map-sequence-id-464623

    Enlarge

  12. pLX_TRC311/NLS-Cas13d-P2A-Blast

    Plasmid
    #212196
    Purpose
    CasRx (NLS-RfxCas13d-NLS) with 2A-Blasticidin for RNA targeting. CasRx-2A-Blasticidin is driven by EF1a promoter. EGFP is driven by SV40
    Depositor
    Stacey Edwards
    Article
    Wang et al Mol Cancer. 2024 May 15;23(1):101. doi:
    Insert
    CasRx (NLS-RfxCas13d-NLS)-HA with 2A-Blasticidin
    Use
    CRISPR and Lentiviral
    Tags
    HA, NLS, and blasticidin S deaminase
    Expression
    Mammalian
    Mutation
    Promoter
    EF-1α promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    212196-plasmid-map-sequence-id-420116

    Enlarge

  15. pAAV-EFS-DjCas13d-P2A-mCherry-pA

    Plasmid
    #233027
    Purpose
    To Express HA tagged DjCas13d and mcherry from the mammalian EFS promoter. The DjCas13dx and mCherry are separated by a P2A site
    Depositor
    Jonathan Ploski
    Insert
    DjCas13d/mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233027-plasmid-map-sequence-id-463789

    Enlarge

  16. pAAV-EFS-hfCas13d-P2A-mCherry-pA

    Plasmid
    #233026
    Purpose
    To Express HA Tagged hfCas13d and mcherry from the mammalian EFS promoter. The hfCas13d and mCherry are separated by a P2A site
    Depositor
    Jonathan Ploski
    Insert
    hfCas13d/mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mutation
    Promoter
    Available since
    March 4, 2025
    Availability
    Academic Institutions and Nonprofits only
    233026-plasmid-map-sequence-id-463769

    Enlarge

Showing: 21 - 40 of 188 results