We narrowed to 175 results for: donor sgrna

1. pUC57-Amp_CRISPIE donor B1

   Plasmid

   #172842

   Purpose

   CRISPIE donor B1 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mEGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC57-Amp_CRISPIE donor B3

   Plasmid

   #172844

   Purpose

   CRISPIE donor B3 (Zhong et al, eLife 2021), mTurquoise2 translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mTurquoise2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC57-Amp_CRISPIE donor B4

   Plasmid

   #172845

   Purpose

   CRISPIE donor B4 (Zhong et al, eLife 2021), mNeonGreen translational phase (0-0), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-0) encoding mNeonGreen
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Intron-Tagging-EGFP-Donor

   Plasmid

   #159740

   Purpose

   Contains EGFP flanked by a splice acceptor and a splice donor. Together with other intron tagging plasmids, it can be used to place the EGFP tag as a synthetic exon into introns of target genes.

   Depositor

   Stefan Kubicek

   Article

   Reicher et al Genome Res. 2020 Dec;30(12):1846-1855. d

   Insert

   EGFP
   

   Use

   Intron tagging donor

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC57-Amp_CRISPIE donor B5

   Plasmid

   #172846

   Purpose

   CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV_Donor/Reporter-COL4A5

   Plasmid

   #130280

   Purpose

   COL4A5 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A5 donor template

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   mCherry-eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV_Donor/Reporter-COL4A3

   Plasmid

   #130279

   Purpose

   COL4A3 mutation-specific sgRNA under the control of U6 promoter; mCherry-sgRNA target-(out of frame)EGFP expression cassette; COL4A3 donor template

   Depositor

   Silvestro Conticello

   Article

   Daga et al Eur J Hum Genet. 2019 Nov 21. pii: 10.10

   Insert

   mCherry-eGFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. sgDonor_lentiGuide

   Plasmid

   #184168

   Purpose

   LentiGuide-puro vector expressing the sgRNA necessary for linearization of the donor cassette plasmid.

   Depositor

   Ophir Shalem

   Article

   Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

   Insert

   sgDonor
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAVS1_Puro_hPGK1_EGFP_Donor

   Plasmid

   #178088

   Purpose

   HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

   Depositor

   Yannick Doyon

   Article

   Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

   Insert

   EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human PGK1

   Available since

   Dec. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1_Puro_hPGK1_EBFP_Donor

    Plasmid

    #178089

    Purpose

    HDR donor plasmid to introduce the EBFP (T65S/F145Y) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    EBFP (T65S/F145Y)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAVS1_Puro_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid

    #178087

    Purpose

    HDR donor plasmid to introduce the mScarlet-I mTOR signaling indicator (TOSI) cassette to AAVS1 using AAVS1 T2 sgRNA.

    Depositor

    Yannick Doyon

    Article

    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

    Insert

    mScarlet-I mTOR signaling indicator (TOSI)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human PGK1

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-sp-sgRNA-mDNMT1_nick-lenti

    Plasmid

    #135959

    Purpose

    S. pyogenes nicking sgRNA for mouse DNMT1

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    mDNMT1_nick
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pU6-sp-sgRNA-RNF2_+41nick

    Plasmid

    #135958

    Purpose

    S. pyogenes sgRNA for +41 nick in RNF2

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    RNF2_+41 nicking sgRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available since

    Jan. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. KL060 - pBlueScript II SK-mAID-EGFP CTCF donor

    Plasmid

    #232926

    Purpose

    Donor plasmid for tagging mouse CTCF with mAID-eGFP at the N-terminus. Used with KL061 - PX330-sgRNA006_CTCF sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    mAID-eGFP with mCTCF homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. eGFPbait-E2A-KalTA4-pA donor vector

    Plasmid

    #61069

    Purpose

    for CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)

    Depositor

    Filippo Del Bene

    Article

    Auer et al Genome Res. 2014 Jan;24(1):142-53. doi:

    Inserts

    eGFPbait
    KalTA4
    

    Use

    Zebrafish expression

    Tags

    E2A

    Expression

    Mutation

    Promoter

    Available since

    Feb. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

    Plasmid

    #232936

    Purpose

    Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

    Depositor

    Danny Reinberg

    Article

    Lucero et al bioRxiv, March 19, 2025

    Insert

    T2A-eGFP-SV40pA with Podxl homology arms
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pU6_B2M_sgRNA(PP7)

    Plasmid

    #232438

    Purpose

    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell, April 2025. doi: 10.1016/j.cell.20

    Insert

    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA1

    Plasmid

    #117405

    Purpose

    SpyCas9 sgRNA 1 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLKO.1-puro-U6-SpyCas9-TLR-MCV1-sgRNA2

    Plasmid

    #117406

    Purpose

    SpyCas9 sgRNA 2 targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    SpyCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLKO.1-puro-U6-NmeCas9-TLR-MCV1-sgRNA

    Plasmid

    #117407

    Purpose

    NmeCas9 sgRNA targeting TLR2.0

    Depositor

    Erik Sontheimer

    Article

    Iyer et al CRISPR J. 2022 Oct;5(5):685-701. doi: 10

    Insert

    NmeCas9 sgRNA targeting TLR 2.0
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge