Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 593 results for: pFAST

Showing: 21 - 40 of 593 results

  1. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

    Enlarge

  2. pFastBac_Dual_SNF2L-opt_p48

    Plasmid
    #102243
    Purpose
    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual
    Depositor
    Joe Landry
    Inserts
    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    codon optimized for expression
    Promoter
    Polyhedrin and p10
    Available since
    Nov. 1, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102243-plasmid-map-sequence-id-197769

    Enlarge

  3. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Article
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Use
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + T…
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186626-plasmid-map-sequence-id-366906

    Enlarge

  4. pFastBac1_FLAG-BPTF

    Plasmid
    #102242
    Purpose
    Expression of human Flag-BPTF using baculovirus from pFastBac1
    Depositor
    Joe Landry
    Insert
    Flag-BPTF (BPTF Human)
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    Polyhedron
    Available since
    Dec. 11, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102242-plasmid-map-sequence-id-197767

    Enlarge

  8. pEND-164_Cacnes_pFAST

    Plasmid
    #225616
    Purpose
    Replicative vector for anaerobic fluorescent reporter protein FAST tag strong constitutive recombinant expression in C. acnes. pBRESP36A with P(BBa_J23119)+RBS_1+pFAST.
    Depositor
    Marc Güell
    Article
    Guillermo et al Cell Systems, 2025
    Insert
    pFAST
    Use
    Synthetic Biology
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    Codon optimized for Cutibacterium acnes
    Promoter
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    225616-plasmid-map-sequence-id-451365

    Enlarge

  13. pFastBac HT JS-Rab10wt

    Plasmid
    #135557
    Purpose
    Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.
    Depositor
    Jingshi Shen
    Article
    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11
    Insert
    Rab10 (Rab10 Mouse)
    Use
    Tags
    6xHis tag, TEV site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135557-plasmid-map-sequence-id-272501

    Enlarge

  19. pFastBac1-Nucleocapsid

    Plasmid
    #179696
    Purpose
    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells
    Depositor
    Mark Arbing
    Insert
    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His6-TEV protease site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179696-plasmid-map-sequence-id-352199

    Enlarge

Showing: 21 - 40 of 593 results