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We narrowed to 596 results for: pFAST

Showing: 21 - 40 of 596 results

  1. pFastBac1-melHis10TEV

    Plasmid
    #159428
    Purpose
    empty pFastBac1 secretion vector (polyhedrin promoter) adds N-term melittin signal sequence & TEV-protease-removable His10; avoids unwanted residues in final protein by using BseRI restriction sites
    Depositor
    Debra Hansen
    Type
    Empty backbone
    Use
    Tags
    melittin signal sequence (removed in vivo by sign…
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    159428-plasmid-map-sequence-id-333931

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  2. pFastBac1-His10FLAG

    Plasmid
    #157736
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds enterokinase-removable N-terminal His10-tag + 3xFLAG tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    His10 + 3xFLAG, enterokinase-cleavable
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    157736-plasmid-map-sequence-id-333926

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  3. pFastBac HT JS-Munc18c

    Plasmid
    #135555
    Purpose
    Express mouse Munc18c in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Munc18c.
    Depositor
    Jingshi Shen
    Article
    Yu et al Methods Mol Biol. 2019;1860:237-249. doi
    Insert
    Munc18c
    Use
    Tags
    6xHis tag and TEV site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135555-plasmid-map-sequence-id-272498

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  4. pFastBac_Dual_SNF2L-opt_p48

    Plasmid
    #102243
    Purpose
    Expression of human codon optimized human SNF2L and pRbAp48 from pFastBac dual
    Depositor
    Joe Landry
    Inserts
    SNF2L (SMARCA1 Human)
    pRbAp48 (RBBP4 Human)
    Use
    Tags
    Expression
    Insect
    Mutation
    codon optimized for expression
    Promoter
    Polyhedrin and p10
    Available since
    Nov. 1, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102243-plasmid-map-sequence-id-197769

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  5. pFastBac1-His10TEV

    Plasmid
    #159427
    Purpose
    empty pFastBac1 vector (polyhedrin promoter) adds TEV-protease-removable N-terminal His10-tag; avoids unwanted residues in the final protein sequence by using the BseRI restriction sites
    Depositor
    Debra Hansen
    Article
    Olson et al Protein Expr Purif. 2021 May 7:105890. d
    Type
    Empty backbone
    Use
    Tags
    TEV-protease-cleavable His10-tag
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    June 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    159427-plasmid-map-sequence-id-333995

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  6. pFastBac1_FLAG-BPTF

    Plasmid
    #102242
    Purpose
    Expression of human Flag-BPTF using baculovirus from pFastBac1
    Depositor
    Joe Landry
    Insert
    Flag-BPTF (BPTF Human)
    Use
    Tags
    Flag
    Expression
    Insect
    Mutation
    Promoter
    Polyhedron
    Available since
    Dec. 11, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    102242-plasmid-map-sequence-id-197767

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  9. pFastBac1-pGC-A

    Plasmid
    #186626
    Purpose
    pFastBac1 (baculovirus polyhedrin promoter) encoding HA signal peptide, TEV-protease cleavable His10-tag, and full-length human pGC-A (NP_000897.3 amino acids 33-1061; expression-optimized DNA)
    Depositor
    Debra Hansen
    Article
    Zhang et al Sci Rep. 2022 Jul 12;12(1):11824. doi: 1
    Insert
    NPR1 (NPR1 Human)
    Use
    Tags
    hemagglutinin (HA) signal peptide + His10-tag + T…
    Expression
    Insect
    Mutation
    deleted amino acids 1-32
    Promoter
    polyhedring
    Available since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    186626-plasmid-map-sequence-id-366906

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  15. pFastBac HT JS-Rab10wt

    Plasmid
    #135557
    Purpose
    Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.
    Depositor
    Jingshi Shen
    Article
    Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11
    Insert
    Rab10 (Rab10 Mouse)
    Use
    Tags
    6xHis tag, TEV site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin promoter
    Available since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    135557-plasmid-map-sequence-id-272501

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  19. pFastBac1-Nucleocapsid

    Plasmid
    #179696
    Purpose
    Expresses H6 tagged SARS CoV-2 nucleocapsid in Sf9 cells
    Depositor
    Mark Arbing
    Insert
    SARS-CoV-2 Nucleocapsid (N protein) (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His6-TEV protease site
    Expression
    Insect
    Mutation
    Promoter
    polyhedrin
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    179696-plasmid-map-sequence-id-352199

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Showing: 21 - 40 of 596 results