2,366 results

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pFudio-Cpg15-IRES2-TdTomatoW

Plasmid

#134309

Purpose

Expresses cpg15 and TdTomato upon coexpression with Cre recombinase

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Inserts

cpg15 (Nrn1 Rat, Mouse)
TdTomato

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

UbC

Available Since

May 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-OTp-GCaMP6s

Plasmid

#192945

Purpose

To drive GCaMP6s under the control of mouse oxytocin promoter

Depositor

Kazunari Miyamichi

Article

Yaguchi et al PLoS One. 2023 May 10;18(5):e0285589. do

Insert

GCaMP6s

Use

AAV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCF CREB

Plasmid

#22968

Depositor

Marc Montminy

Article

Du et al Mol Cell Biol. 2000 Jun . 20(12):4320-7.

Insert

CREB (Creb1 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG-S6K1

Plasmid

#100509

Purpose

Expresses FLAG tagged S6K1 in mammalian cells

Depositor

David Sabatini

Article

Gu et al Science. 2017 Nov 10;358(6364):813-818.

Insert

S6K1 (Rps6kb1 Rat)

Tags

FLAG

Expression

Mammalian

Available Since

Nov. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-Synapsin-pHoenix-WPRE

Plasmid

#70111

Purpose

lentiviral expression of pHoenix (Synaptophysin-pHluorin-Arch3-mKate2) driven by human Synapsin I promoter for optogenetic acidification of synaptic vesicles

Depositor

Christian Rosenmund

Article

Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

Insert

pHoenix (Syp Rat, Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria)

Use

Lentiviral

Tags

Arch3, H+/K+ ATPase beta-subunit, mKate2, and pHl…

Promoter

Synapsin

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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G protein alpha-s-GFP

Plasmid

#66083

Purpose

G protein alpha-s internally tagged with EGFP and EE epitope

Depositor

Catherine Berlot

Article

Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

Insert

G-alpha-s-EE-EGFP (Gnas Rat)

Tags

EGFP flanked by SGGGS on each side was inserted i…

Expression

Mammalian

Mutation

BamHI sites in the alpha-s cDNA were removed and …

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-H2BmGreenLantern

Plasmid

#177332

Purpose

To express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.

Depositor

Murray Blackmore, Gregory Petsko, Pantelis Tsoulfas

Article

Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi:

Insert

H2B (Hist1h2bq Rat)

Use

AAV

Tags

mGreenLantern

Expression

Mammalian

Mutation

This fusion protein was optimized to the Human co…

Promoter

CAG

Available Since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-mCherry-HPos

Plasmid

#87159

Purpose

Fluorescent marker of lipid droplets (LDs)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

HPos

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-C1-CAMKIIbeta

Plasmid

#21227

Depositor

Tobias Meyer

Article

Shen et al Neuron. 1998 Sep . 21(3):593-606.

Insert

CAMKIIbeta (Camk2b Rat)

Tags

GFP

Expression

Mammalian

Mutation

Lab plasmid KS 0002.

Available Since

July 10, 2009

Availability

Academic Institutions and Nonprofits only

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pCS2+-H2B-pr-mEosFP

Plasmid

#141101

Purpose

H2B fused to pr-mEosFP for chromatin visualization and primed conversion

Depositor

Periklis Pantazis

Article

Welling et al Bio Protoc. 2020 Jun 5;10(11):e3645. doi

Insert

Histone 2B

Tags

pr-mEosFP

Expression

Mammalian

Promoter

simian CMV IE94

Available Since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin2b KI

Plasmid

#131487

Purpose

Endogenous tagging of GluN2b: N-terminal (amino acid position: S34)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Grin2b Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pmcherry-LCa

Plasmid

#53972

Purpose

Expresses the light chain of clathrin

Depositor

Tom Kirchhausen

Article

Kural et al Cell Rep. 2012 Nov 29;2(5):1111-9. doi:

Insert

clathrin light chain

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2014

Availability

Academic Institutions and Nonprofits only
pGP-AAV-syn-jGCaMP7s-WPRE

Plasmid

#104487

Purpose

AAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter

Depositor

Douglas Kim, GENIE Project

Article

Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

Has Service

AAV PHP.eB, AAV Retrograde, AAV1, and AAV9

Insert

jGCaMP7s

Use

AAV

Tags

T7 epitope, Xpress tag, 6xHis

Expression

Mammalian

Promoter

Synapsin

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pCI-syn-fRGECO1

Plasmid

#125243

Purpose

Expresses red fluorescent calcium sensor in hippocampal slices

Depositor

Katalin Torok

Article

Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

Insert

fRGECO1

Expression

Mammalian

Mutation

D323A

Promoter

synapsin

Available Since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLAMP1-miRFP670nano3

Plasmid

#184669

Purpose

LAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

LAMP1-miRFP670nano3

Tags

miRFP670nano3

Expression

Mammalian

Promoter

CMV

Available Since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-i3 ONE-GO

Plasmid

#189737

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-i3 ONE-GO
GNAI3 (Gnai3 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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msfGFP-Cx43

Plasmid

#69024

Purpose

Expresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to msfGFP (FP fused to NT of connexin). CMV promoter.

Depositor

David Spray

Article

Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

Insert

Cx43 (Gja1 Rat)

Tags

sfGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-tdTomato-P2A-paCaMKII

Plasmid

#165431

Purpose

Expresses tdTomato and paCaMKII in mammalian cells to induce synaptic plasticity.

Depositor

Hideji Murakoshi

Article

Shibata et al Nat Commun. 2021 Feb 2;12(1):751. doi: 1

Insert

tdTomato-P2A-paCaMKII

Tags

tdTomato

Expression

Mammalian

Promoter

CMV

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-GRASP55

Plasmid

#137708

Purpose

Eukaryotic expression of rat GRASP55 full-length

Depositor

Yanzhuang Wang

Insert

GRASP55

Tags

EGFP

Expression

Mammalian

Available Since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-mCREB

Plasmid

#68551

Purpose

AAV expression of mCREB

Depositor

Eric Nestler

Article

Berger et al Biol Psychiatry. 2011 Feb 1;69(3):288-94

Insert

mCREB

Use

AAV

Tags

EGFP

Expression

Mammalian

Mutation

S133A

Promoter

CMV

Available Since

Sept. 2, 2015

Availability

Academic Institutions and Nonprofits only