2,293 results

1. pHAGE pGK LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215015

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Available Since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-GCaMP6fu

   Plasmid

   #86235

   Purpose

   Express fluorescent calcium indicator GCaMP6fu in mammalian cells

   Depositor

   Katalin Torok

   Article

   Helassa et al Sci Rep. 2016 Dec 6;6:38276. doi: 10.103

   Insert

   GCaMP6fu
   

   Expression

   Mammalian

   Mutation

   W43Y D395A

   Promoter

   CMV

   Available Since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV GFAP jGCaMP7b

   Plasmid

   #171118

   Purpose

   Astrocyte AAV-mediated expression of bright ultrasensitive protein calcium sensor under the GFAP promoter

   Depositor

   Thomas Oertner

   Article

   Lohr et al Front Cell Neurosci. 2021 Jun 11;15:6901

   Insert

   jGCaMP7b
   

   Use

   AAV

   Tags

   6 His Tag, T7 Tag, and Xpress Tag

   Expression

   Mammalian

   Promoter

   GFAP

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1-PRKAA1

   Plasmid

   #30305

   Depositor

   Jay Brenman

   Article

   Kazgan et al Mol Biol Cell. 2010 Oct 1. 21(19):3433-4

   Insert

   AMP-activated Protein Kinase α1 (Prkaa1 Rat)
   

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMRXIB-mRuby3-LC3

   Plasmid

   #221738

   Purpose

   Stable expression of mRuby3-LC3 in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   LC3B (Map1lc3b Rat)
   

   Use

   Retroviral

   Tags

   mRuby3

   Expression

   Mammalian

   Available Since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI pHluorin Syt12

   Plasmid

   #184513

   Purpose

   Mammalian expression of Synaptotagmin 12 with pH-sensitive fluorescent protein

   Depositor

   Edwin Chapman

   Article

   Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

   Insert

   Synaptotagmin 12 (Syt12 Rat)
   

   Tags

   Super Ecliptic pHluorin

   Expression

   Mammalian

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pl-Synapsin-YFP-NaVII-III

   Plasmid

   #91246

   Purpose

   Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)

   Depositor

   Matthew Grubb

   Article

   Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.

   Insert

   Nav1.2 (Scn2a Rat)
   

   Use

   Lentiviral

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   The insert codes only for the intracellular loop …

   Available Since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. mEmerald-Cx43-7

   Plasmid

   #54055

   Purpose

   Localization: Gap Junctions, Excitation: 487, Emission: 509

   Depositor

   Michael Davidson

   Article

   Fong et al Autophagy. 2012 May 1;8(5):794-811. doi:

   Insert

   Cx43
   

   Tags

   mEmerald

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 2, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pVQ CMV NanoV1Mutant-2a-EGFP ferritin

   Plasmid

   #79650

   Purpose

   Expresses camelid anti-GFP nanobody fused to TRPV1 with mutant pore region and GFP-ferritin chimera fusion protein

   Depositor

   Jeffrey Friedman

   Article

   Stanley et al Nature. 2016 Mar 31;531(7596):647-50. do

   Inserts

   transient receptor potential vanilloid 1 (Trpv1 Rat)
   camelid anti-GFP nanobody
   Enhanced green fluorescent protein
   mouse ferritin light chain (Ftl1 Mouse)
   mouse ferritin heavy chain (Fth1 Mouse)
   

   Use

   Adenoviral

   Expression

   Mammalian

   Mutation

   isoleucine 679 changed to lysine

   Promoter

   CMV

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-TRE-GCaMP6s

    Plasmid

    #135418

    Purpose

    Can be used to drive amplified GCaMP6s expression when combined with TET driver . Can be used to create adeno-associated virus to express TET-driven GCaMP6s.

    Depositor

    Rylan Larsen

    Insert

    GCaMP6s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-HA-rat Nrxn3beta AS4(-)

    Plasmid

    #58269

    Purpose

    Expression plasmid for HA-tagged rat Neurexin 3beta, lacking the insert at alternatively spliced segment 4 (AS4-)

    Depositor

    Peter Scheiffele

    Article

    Taniguchi et al J Neurosci. 2007 Mar 14;27(11):2815-24.

    Insert

    Nrxn3beta AS4(-) (Nrxn3 Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHTN-LC3B

    Plasmid

    #219440

    Purpose

    Expresses HaloTag-LC3B, an autophagosome marker

    Depositor

    Erika Holzbaur

    Article

    Tudorica et al J Cell Biol. 2024 Jul 1;223(7):e20230901

    Insert

    LC3B (Map1lc3b Rat)
    

    Tags

    HaloTag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGP-CMV-jGCaMP7c variant 1513

    Plasmid

    #105320

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    GENIE Project, Douglas Kim

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7c variant 1513
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBK-VRER-BE3

    Plasmid

    #85173

    Purpose

    Expresses VRER-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VRER-BE3
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-CIB1-mCerulean-MP

    Plasmid

    #58366

    Purpose

    For use in light-induced protein inactivation through the interaction with CRY2

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Inserts

    CIB1[1-147] (CIB1 Mustard Weed)
    Camk2a[315-478] (Camk2a Rat)
    

    Tags

    mCerulean

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV-MPAct-mRuby3-IRES-Puro

    Plasmid

    #155231

    Purpose

    lentiviral MPAct construct

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    Lentiviral expression of MPAct-mRuby sensor
    

    Use

    Lentiviral

    Tags

    mRuby3

    Available Since

    Aug. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-AU1-mTOR-S2215Y

    Plasmid

    #26037

    Depositor

    Fuyuhiko Tamanoi

    Article

    Sato et al Oncogene. 2010 May 6. 29(18):2746-52.

    Insert

    mammalian target of rapamycin-S2215Y (Mtor Rat)
    

    Tags

    AU1

    Expression

    Mammalian

    Mutation

    S2215Y

    Available Since

    Sept. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pFsynW SYP-GFP

    Plasmid

    #197283

    Purpose

    Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

    Depositor

    Edwin Chapman

    Article

    Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

    Insert

    SYP and GFP (Syp Rat)
    

    Use

    Lentiviral

    Tags

    1D4 epitope

    Mutation

    N/A

    Promoter

    hSyn (human synapsin I promoter)

    Available Since

    July 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBiFC-bJunVN155(I152L)

    Plasmid

    #27098

    Depositor

    Chang-Deng Hu

    Article

    Kodama et al Biotechniques. 2010 Nov . 49(5):793-805.

    Insert

    bJunVN155(I152L) (Jun Rat, and Aequorea Victoria)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    I152L in Venus(1-154)

    Available Since

    Jan. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCI-SEP-GluR2(Q)

    Plasmid

    #24002

    Depositor

    Robert Malinow

    Article

    Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

    Insert

    GluR2 (Gria2 Rat)
    

    Tags

    GluR2 signal peptide (aa1-24) and SEP (supereclip…

    Expression

    Mammalian

    Mutation

    R607Q

    Promoter

    CMV

    Available Since

    April 6, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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