Addgene

We narrowed to 1,192 results for: 5-Oct

Showing: 571 - 600 of 1192 results

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CAG-BFP-sesRNA-tTA2-WPRE-pA

Plasmid

#192070

Purpose

Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for tdTom

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Feb. 2, 2023

Availability

Academic Institutions and Nonprofits only

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CAG-ADAR2-sesRNA-GFP-W3SL

Plasmid

#192069

Purpose

Expression of ADAR2, sesRNA in mammalian cells, with tTA2 as efRNA and ADAR2 overexpression.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for mouse Ctip2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Cbh

Available since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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His-MBP_G4G6 in pRSF

Plasmid

#188454

Purpose

Express in E. coli human gelsolin segments 4 through 6 with N-terminal 6xHis and MBP tags.

Depositor

Roberto Dominguez

Article

Ceron et al Proc Natl Acad Sci U S A. 2022 Oct 11;11

Insert

G4G6 (GSN Human)

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

WT

Promoter

T7

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgTK2_5

Plasmid

#217432

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human TK2

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 5 targeting TK2 (TK2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pSEED

Plasmid

#219658

Purpose

Permits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Empty SEED Cassette (3xP3-dsRed)

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP

Plasmid

#219639

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox5

Plasmid

#69354

Purpose

expresses human Nox5 in mammalian cells

Depositor

Karl-Heinz Krause

Article

Banfi et al J Biol Chem. 2001 Oct 5;276(40):37594-60

Insert

hNox5 (NOX5 Human)

Use

Tags

no

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pSEED-mGreenLantern:ALFA

Plasmid

#219644

Purpose

Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mScarlet:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mGreenLantern:ALFA SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-mCherry

Plasmid

#219657

Purpose

Permits GoldenGate (BsmBI) assembly of donor plasmids to knock-in mCherry using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-mScarlet3

Plasmid

#219640

Purpose

Permits GoldenGate assembly of donor plasmids to knock-in mScarlet3 (BsmBI) using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mScarlet SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-Dendra2

Plasmid

#219645

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Dendra2 using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

Dendra2 SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-FOXB1

Plasmid

#153112

Purpose

Expression of Flag tagged human FOXB1 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXB1 (FOXB1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pSEED-OLLAS:HA

Plasmid

#219647

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in OLLAS and HA tags using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

OLLAS:HA SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5_T

Plasmid

#147552

Purpose

Mammalian Expression of HsSmg5

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5 (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation compared to the sequence give…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSEED-mScarlet3:ALFA

Plasmid

#219641

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in mScarlet3:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mScarlet:ALFA SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-sfGFP:ALFA

Plasmid

#219642

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP:ALFA using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP:ALFA SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pWalium20-10XUAS-3XFLAG-dCas9-VPR

Plasmid

#78897

Purpose

Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

Depositor

Norbert Perrimon

Article

Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

Insert

dCas9-VPR

Use

Tags

3xFLAG

Expression

Insect

Mutation

Promoter

10XUAS

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pmCherry-T2A-moxMaple3

Plasmid

#120875

Purpose

Mammalian expression of mCherry and moxMaple3

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Inserts

mCherry
moxMaple3

Use

Tags

Expression

Mammalian

Mutation

from mMaple3, moxMaple3 was engineered by replaci…

Promoter

Available since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pSEED-mGreenLantern

Plasmid

#219643

Purpose

Permits GoldenGate assembly of donor plasmids (BsmBI) to knock-in mGreenLantern using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

mGreenLantern SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-histone H3

Plasmid

#179699

Purpose

visualization of nucleus/chromosome

Depositor

Kenji Sugimoto

Article

Yasui et al Mutat Res. 2010 Oct 13;692(1-2):12-8. do

Insert

Histone H3 (Hist1h3h Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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Htb2-GFP

Plasmid

#117669

Purpose

Colorize the nuclei

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

Htb2-GFP

Use

Tags

Expression

Yeast

Mutation

Contains the gene encoding the Histone Htb2 from …

Promoter

pGAP

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pKar2-msGFP-HDEL

Plasmid

#117668

Purpose

Localisation of Endoplasmic Reticulum

Depositor

Pau Ferrer, Benjamin Glick

Article

Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

Insert

pKar2-msGFP-HDEL

Use

Tags

Expression

Yeast

Mutation

Contains the Kar2 promoter and signal sequence fr…

Promoter

pKar2

Available since

March 18, 2019

Availability

Academic Institutions and Nonprofits only

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pMTTH+bromo5

Plasmid

#157803

Purpose

Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

bromo5

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

pTAC

Available since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-Dlx5

Plasmid

#74344

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Dlx5. Not for gene expression.

Depositor

Peter Mombaerts

Article

Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

Insert

distal-less homeobox 5 (Dlx5 Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

May 9, 2016

Availability

Academic Institutions and Nonprofits only

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Flag-FOXD4

Plasmid

#153119

Purpose

Expression of Flag tagged human FOXD4 in mammalian cells

Depositor

Stefan Koch

Article

Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

Insert

human FOXD4 (FOXD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSEED-Moontag:HA

Plasmid

#219648

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in Moontag and HAtag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

MoonTag:HA SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pUASTattB-degradALFA

Plasmid

#219663

Purpose

Permits Gal4-inducible expression of degradALFA, a fusion of anti-ALFANb to the F-Box of Slmb.

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

degradALFA

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pSEED-EYFP:HA

Plasmid

#219656

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in EYFP and HA tag using the SEED/Harvest method

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

EYFP:HA SEED Cassette

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Atg11 [699-800] pHis2

Plasmid

#190264

Purpose

Codon optimized Atg11 amino acids 699-800 from S. cerevisiae for expression in E. coli.

Depositor

Michael Ragusa

Article

Margolis et al J Mol Biol. 2020 Oct 2;432(21):5752-5764

Insert

ATG11

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsSmg5-Q123E_T

Plasmid

#147555

Purpose

Mammalian Expression of HsSmg5-Q123E

Depositor

Elisa Izaurralde

Article

Loh et al Genes Dev. 2013 Oct 1;27(19):2125-38. do

Insert

HsSmg5-Q123E (SMG5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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