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Showing: 761 - 800 of 25998 results

  3. pKM343

    Plasmid
    #71487
    Purpose
    Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.
    Depositor
    Kenan Murphy
    Article
    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:
    Insert
    loxP-hyg-cat-loxP
    Use
    Source of loxp-hyg-cat- loxp for pcr amplificatio…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    June 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    71487-plasmid-map-sequence-id-210536

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  5. pKM402

    Plasmid
    #107770
    Purpose
    Expression of phage Che9c RecT for oligo-mediated recombineering in mycobacteria
    Depositor
    Kenan Murphy
    Article
    Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:
    Inserts
    Che9C RecT
    TetR (tet repressor)
    cat
    kan
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Cat promoter, Ptet, kan promoter, and pTB21
    Available since
    May 15, 2018
    Availability
    Academic Institutions and Nonprofits only
    107770-plasmid-map-sequence-id-210549

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  8. pAH25-SceI

    Plasmid
    #129389
    Purpose
    Derived from pDAI-SceI. Tetracycline resistance removed and replaced with chloramphenicol resistance cassette from pKD3.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Chloramphenicol acetyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    N/A
    Promoter
    CAT promoter
    Available since
    Oct. 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    129389-plasmid-map-sequence-id-258595

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  9. pSAH0016

    Plasmid
    #79824
    Purpose
    Dual selection plasmid with lac operator, selection for binding with spectinomycin, for non-binding with chloramphenicol
    Depositor
    Katja Arndt
    Article
    Hoffmann et al Nucleic Acids Res. 2016 Jun 2;44(10):e95
    Inserts
    cat
    aadA
    Use
    Tags
    Expression
    Mutation
    Promoter
    AATTCAAAAAAATGCTTGACAATTAATCATCGGCTCGTATAATGTGTGG…
    Available since
    Aug. 2, 2016
    Availability
    Academic Institutions and Nonprofits only
    79824-plasmid-map-sequence-id-149510

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  11. pJAB988

    Plasmid
    #133920
    Purpose
    miniICE-tet(M)cat(PC194)_PT7-GFP; used to integrate into JAB1000 B. subtilis XPORT strain with IPTG-inducible GFP under T7 promoter
    Depositor
    Christopher Voigt
    Article
    Brophy et al Nat Microbiol. 2018 Sep;3(9):1043-1053.
    Insert
    miniICE-tet(M)cat(PC194)_PT7-GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    133920-plasmid-map-sequence-id-409802

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  13. pMflCT-o4

    Plasmid
    #101313
    Purpose
    Contains: complete oriC region of M. florum L1 (oriC4 fragment), colE1 rep origin, recoded tetM and cat resistance genes, origin of transfer of RP4 plasmid.
    Depositor
    Sébastien Rodrigue
    Article
    Matteau et al Appl Environ Microbiol. 2017 Mar 17;83(7
    Inserts
    oriC4 of M. florum strain L1 (rpmH/dnaA and dnaA/dnaN intergenic regions, with dnaA)
    cat resistance gene
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    101313-plasmid-map-sequence-id-199224

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  15. pKD3_mCherry

    Plasmid
    #187385
    Purpose
    To chromosomally tag genes of interest with mCherry. FRT-mCherry-cat-FRT
    Depositor
    Roi Avraham
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Sept. 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    187385-plasmid-map-sequence-id-371123

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  17. pDONR221-SLC7A3_STOP

    Plasmid
    #161467
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC7A3 (SLC7A3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161467-plasmid-map-sequence-id-355126

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  18. pDONR221-SLC7A4_STOP

    Plasmid
    #161351
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC7A4 (SLC7A4 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 15, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161351-plasmid-map-sequence-id-416388

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  22. pKAN43Ag8-GFP

    Plasmid
    #178182
    Purpose
    Plant binary vector expressing GFP by a enhanced 35S promoter
    Depositor
    Keiichi Shimizu
    Insert
    EGFP (eGFP )
    Use
    Tags
    Expression
    Plant
    Mutation
    The CAT is inserted after the start codon.
    Promoter
    The minimal 35S promoter from Cauliflower Mosaic …
    Available since
    Sept. 26, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    178182-plasmid-map-sequence-id-446882

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  23. pBABE zeo Ecotropic Receptor

    Plasmid
    #10687
    Depositor
    William Hahn
    Insert
    Ecotropic Receptor (Slc7a1 Mouse)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 7, 2005
    Availability
    Academic Institutions and Nonprofits only

  24. pDONR221-SLC7A1_STOP

    Plasmid
    #161091
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Article
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Insert
    SLC7A1 (SLC7A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    161091-plasmid-map-sequence-id-353417

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  26. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

    Plasmid
    #102277
    Purpose
    For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
    Depositor
    Samie Jaffrey
    Article
    Kan et al Nat Commun. 2017 Jul 4;8:15737. doi: 10.
    Insert
    YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
    Use
    For mammalian cell-free protein expression system…
    Tags
    GST, HA, and HIS
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    102277-plasmid-map-sequence-id-197159

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  28. CECR2

    Plasmid
    #39066
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CECR2 (CECR2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  32. pattB-aubergine-ADH-gf

    Plasmid
    #69448
    Purpose
    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub
    Depositor
    Phillip Zamore
    Insert
    aubergine (aub Fly)
    Use
    Tags
    NA
    Expression
    Insect
    Mutation
    chr2L:10,998,626(−)T>G
    Promoter
    aubergine
    Available since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69448-plasmid-map-sequence-id-122550

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  33. pET29b-RupA_C1-A1-T1-CHis

    Plasmid
    #109255
    Purpose
    NRPS module containing CAT domains with C-terminal 6His tag
    Depositor
    Emily Balskus
    Article
    Schneider et al Tetrahedron
    Insert
    RupA_C1-A1-T1
    Use
    Tags
    His tag and S-Tag
    Expression
    Bacterial
    Mutation
    contains aa 1..1025 of WP_015523502.1
    Promoter
    T7
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
    109255-plasmid-map-sequence-id-214294

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  34. pSAP

    Plasmid
    #206429
    Purpose
    A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).
    Depositor
    Bogumil Karas
    Article
    Walker et al Plant Physiol. 2023 Dec 19:kiad670. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    206429-plasmid-map-sequence-id-419733

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  36. pSEVA3b61

    Plasmid
    #58310
    Purpose
    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.
    Depositor
    Tom Ellis
    Article
    Wright et al ACS Synth Biol. 2014 May 13.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    58310-plasmid-map-sequence-id-89822

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  37. pG5ElbLuc

    Plasmid
    #89341
    Purpose
    The plasmid pG5ElbLuc was prepared from pG5E1bCAT by replacing the CAT gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745bp StuI-Hind111 fragment.
    Depositor
    Roger Davis
    Article
    Seth et al J Biol Chem. 1992 Dec 5;267(34):24796-80
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
    89341-plasmid-map-sequence-id-176438

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Showing: 761 - 800 of 25998 results