We narrowed to 20,792 results for: ATO
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Plasmid#71128PurposeThis plasmid contains a Cas9 Activator for yeast. The activator is about 1.2-1.8 X more potent than just dCas9-VP64 fusion in yeast. It is on a single copy CEN/ARS plasmid with Ura marker, pRS416.DepositorInsertGal4-dCas9-VP64
TagsNLS n-terminal, N terminal Gal4 Activator domain,…ExpressionYeastPromoterTef1Available SinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
L40C-CRISPR.EFS.mNeon
Plasmid#69146PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), mNeon coexpression, EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
L-CRISPR-CTN (MLL-ENL)
Plasmid#69212PurposeLentiviral CRISPR-Cas9 vector for induction of chromosomal translocations; MLL-ENL,(t[11;19])DepositorInsertsUseCRISPR and LentiviralTagsFLAGAvailable SinceJune 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3_MSR1_bothneg0topos3pos1
Plasmid#232387PurposeMSR1 enhancer, left buffering Coordinator converted to sensitizing double Ebox and right buffering Coordinator to sensitizing CoordinatorDepositorAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:GCaMP6s; cryaa:mCherry
Plasmid#230060PurposeExpression of genetically encoded calcium indicator in zebrafish hepatocytes.DepositorInsertGCaMP6s
UseZebrafish expressionPromoterfabp10aAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
fabp10a:FLAG-SpiCee-mRFP1; cryaa:mCherry
Plasmid#230062PurposeExpression of genetically encoded calcium chelator in zebrafish hepatocytes.DepositorInsertSpiCee
UseZebrafish expressionTagsFLAG and mRFP1Promoterfabp10aAvailable SinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
8xGTIIC-luciferase
Plasmid#34615PurposeYAP/TAZ-responsive synthetic promoter driving luciferase expressionDepositorInsertsynthetic TEAD luciferase reporter
UseLuciferaseAvailable SinceMarch 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB1-PGK-EGFP
Plasmid#204018PurposeHomologous donor template for NFKB1 knockout expressing EGFPDepositorInsertEGFP
UseCRISPRExpressionMammalianPromoterPGKAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUCmini-NFKB2-PGK-EGFP
Plasmid#204020PurposeHomologous donor template for NFKB2 knockout expressing EGFPDepositorInsertEGFP
UseCRISPRExpressionMammalianPromoterPGKAvailable SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEF-TAZ-N-Flag
Plasmid#19025DepositorAvailable SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-Gal4-vp16
Plasmid#171075PurposeExpresses Gal4-vp16 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
TagsHis and VP16 AD (413-490)ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
F-Cdk8
Plasmid#15366DepositorAvailable SinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pRSF_PpRbcLS
Plasmid#229521PurposeExpresses Phymatoceros phymatodes Rubisco large and small subunit (C terminus hexahistidine)DepositorInsertsRubisco large subunit
Rubisco small subunit
TagshexahistidineExpressionBacterialMutationN terminus chloroplast transit peptide truncationPromoterT7Available SinceJune 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-Gal4-p53
Plasmid#171076PurposeExpresses Gal4-p53 fusion protein in bacteriaDepositorInsertGal4 DBD (NEWENTRY Budding Yeast)
TagsHis and p53(1-85)ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHC005 (∆RE)
Plasmid#226199PurposeConjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).DepositorInsertHomology arms for type I restriction-modification system restriction subunit H16_A0006
UseSynthetic BiologyAvailable SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEF-TAZ-N-Flag (S89A)
Plasmid#19026DepositorInsertTAZ (Wwtr1 Mouse)
TagsFlagExpressionMammalianMutationVector has no SV40 origin, S89A (site directed mu…Available SinceAug. 25, 2008AvailabilityAcademic Institutions and Nonprofits only -
Salsa6f
Plasmid#140188PurposeSalsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)DepositorInsertSalsa6f
TagstdTomato-v5-GCaMP6fExpressionMammalianPromoterCMV Immediate EarlyAvailable SinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
F-CDK8L
Plasmid#24762DepositorAvailable SinceMay 14, 2010AvailabilityAcademic Institutions and Nonprofits only -
pUC-STITCH
Plasmid#129535PurposeSTITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.DepositorInsertSTITCH
UseCRISPR; Donor templateAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV Gsk3 sgRNA/GFP
Plasmid#112733PurposeGsk3b targeting gRNA cloned into px552 (SpGuide) plasmid.DepositorInsertGFP
UseAAV and CRISPRExpressionMammalianAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEX-mTAZ-WW (86)
Plasmid#40915DepositorInsertTAZ (Wwtr1 Mouse)
TagsGSTExpressionBacterialMutationContains mTAZ 14-3-3 binding site and WW domain (…PromoterTacAvailable SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pGEX-mTAZ-WW (43)
Plasmid#40916DepositorInsertTAZ (Wwtr1 Mouse)
TagsGSTExpressionBacterialMutationContains mTAZ WW domain (43 amino acids, 122-164)PromoterTacAvailable SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5-F-AF9
Plasmid#49428PurposeMammalian expression of Flag-tagged human AF9DepositorAvailable SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKH3-TRIM28
Plasmid#45569DepositorAvailable SinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEGFP-TRIM28
Plasmid#45568DepositorInsertTRIM28 (TRIM28 Human)
TagsEGFPExpressionMammalianMutationG34S mutation in EGFPPromoterCMVAvailable SinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGEX-5X-TRIM28
Plasmid#45570DepositorAvailable SinceMay 29, 2013AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-Flag-Narg2
Plasmid#49247Purposemammalian expression of flag-tagged human Narg2DepositorAvailable SinceDec. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET19b-hMED9
Plasmid#15365DepositorAvailable SinceAug. 10, 2007AvailabilityAcademic Institutions and Nonprofits only -
pEF-TAZ-N-Flag (-4)
Plasmid#40914DepositorInsertTAZ (Wwtr1 Mouse)
TagsFlagExpressionMammalianMutationVector has no SV40 origin, and lacks the final fo…Available SinceOct. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET41SIII-ATF6(1-373)
Plasmid#171074PurposeExpresses of ATF6α(1-373) in bacteriaDepositorAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChrimsonR-tdT
Plasmid#59169PurposeExpression vector for ChrimsonR-tdTomatoDepositorInsertChrimsonR-tdTomato
TagstdTomatoExpressionMammalianMutationK176RPromoterCAGAvailable SinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQ-NFY-A-GB-HC
Plasmid#171081PurposeCo-expresses of NFY complex in bacteria. The resulting plasmid was used to generate a single expression construct encoding the three subunit NF-Y, with untagged NFYA, GST-NFYB, and 6-His-NFYCDepositorTagsGST and HisExpressionBacterialPromoterT5 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-hTFIIB
Plasmid#171084PurposeExpresses human TFIIB in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TFIIBDepositorAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter 2kb luciferase
Plasmid#8887DepositorAvailable SinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
CMV Flag PAI-RBP
Plasmid#45452DepositorAvailable SinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET-28a (+)-hTBP
Plasmid#171073PurposeExpresses human TBP in bacteria. The resulting plasmid was used to generate a single expression construct encoding a His-tagged TBPDepositorAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A-TAZ S89A
Plasmid#179911PurposeEntry vector of TAZ S89A for gateway coloning.DepositorInsertWW Domain Containing Transcription Regulator 1 (WWTR1 Human)
UseEntry vectorTags3X Flag-tagMutationSerine 89 mutated to AlanineAvailable SinceMarch 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-KIAA0947
Plasmid#49248PurposeMammalian expression of flag-tagged human KIAA0947DepositorAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-Flag-Med26
Plasmid#49250PurposeMammalian expression of flag-tagged Med26DepositorAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn GFP-Fxr1
Plasmid#112732PurposeAAV plasmid that expresses GFP-Fxr1 fusion protein under hSYN promoterDepositorAvailable SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCELF PAI-RBP v3
Plasmid#45507DepositorAvailable SinceJune 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCHC030 (∆CBBp ∆MBH ∆SH)
Plasmid#226205PurposeElectroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).DepositorInsertHomology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
UseSynthetic BiologyMutation∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…Available SinceNov. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-Syn-ChrimsonR-tdT
Plasmid#59171PurposeAAV-mediated expression of ChrimsonR-tdTomato under the Syn promoterDepositorHas ServiceAAV Retrograde, AAV1, AAV5, and AAV9InsertChrimsonR-tdTomato
UseAAVTagstdTomatoExpressionMammalianPromoterSynAvailable SinceAug. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter luciferase delta CRE
Plasmid#8888DepositorInsertPGC-1 alpha promoter dCRE (Ppargc1a Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationSite-directed mutagenesis to remove the CREB bind…Available SinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
PGC-1 alpha promoter luciferase delta MEF
Plasmid#8889DepositorInsertPGC-1 alpha promoter dMEF (Ppargc1a Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationSite-directed mutagenesis to remove the MEF2 bind…Available SinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-NTD
Plasmid#49255PurposeMammalian expression of flag-tagged Med26-NTDDepositorAvailable SinceDec. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-F-Med26-R61A K62A
Plasmid#49254PurposeMammalian expression of flag-tagged human Med26 R61A K62ADepositorAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-TAZ S89A
Plasmid#179910PurposeConstruct for expressing an activated form of TAZ (encoding be gene WWTR1)DepositorInsertWW Domain Containing Transcription Regulator 1 (WWTR1 Human)
Tags3X Flag-tagExpressionMammalianMutationSerine 89 mutated to AlaninePromoterEF1aAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only