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hKCNQ3(WT).ires.mCherry-pcDNA3

Plasmid

#204362

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ3 (KCNQ3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/T7 RNApol

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-SNAP-hCB2_IRES_EGFP

Plasmid

#223506

Purpose

Expression of CB2 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

Depositor

James Frank

Article

Kosar et al J Am Chem Soc. 2023 Jul 19;145(28):15094

Insert

SNAP-tag-CNR2 and EGFP (CNR2 Human, Synthetic)

Use

Tags

SNAP-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pIRES Centrin1 mCherry

Plasmid

#64338

Purpose

Bicistronic expression of mCherry-Centrin1 fusion and Hygro resistance gene

Depositor

Matthieu Piel

Insert

Centrin1

Use

Tags

IRES-Hygro and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 19, 2015

Availability

Academic Institutions and Nonprofits only

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FIRES-PBCS-E670

Plasmid

#228699

Purpose

Sensor cassette FIRE2, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 SENSOR

Use

Synthetic Biology and Unspecified

Tags

Expression

Mammalian

Mutation

Promoter

hpgk

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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FIREC-PBCS-MR3

Plasmid

#228700

Purpose

Control cassette FIRE2, FIRE fluorescent ratiometric sensor

Depositor

Carolyn Sangokoya

Article

Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

Insert

FIRE2 CONTROL

Use

Synthetic Biology and Unspecified

Tags

Expression

Mammalian

Mutation

Promoter

EF1-alpha

Available since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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CMV-SNAP-hCB1_IRES_EGFP

Plasmid

#223505

Purpose

Expression of CB1 N-terminally fused SNAP-tag, with targeting sequence to boost plasma membrane expression. Coupled with EGFP transfection marker

Depositor

James Frank

Insert

SNAP-tag-CNR1 and EGFP (CNR1 Human, Synthetic)

Use

Tags

SNAP-tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-iresFUCCI

Plasmid

#133371

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

Clover-Geminin-IRES-mKO2-Cdt1

Use

Tags

Flag

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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hKCNQ5(WT).ires.mScarlet-pcDNA3

Plasmid

#204361

Purpose

Heterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)

Depositor

Aguan Wei

Article

Wei et al J Neurophysiol. 2022 Jul 1;128(1):40-61.

Insert

KCNQ5 (KCNQ5 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/T7 RNApol

Available since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pEFIRES-P-mTagBFP-KDEL

Plasmid

#87163

Purpose

Fluorescent marker of endoplasmic reticulum (ER)

Depositor

Elina Ikonen

Article

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

mTagBFP-KDEL

Use

Tags

Bip signal peptide (Homo sapiens) and mTagBFP-KDE…

Expression

Mammalian

Mutation

Promoter

EF-1 alpha

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-SHP2WT

Plasmid

#12283

Depositor

Anton Bennett

Article

Kolli et al J Biol Chem. 2004 Jul 9. 279(28):29374-8

Insert

SHP-2WT (PTPN11 Human)

Use

Tags

Myc (not canonical: KLISEEDL)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 11, 2006

Availability

Academic Institutions and Nonprofits only

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pIRES-hrGFP II-mTET1_CD

Plasmid

#83571

Purpose

Expresses a mutant catalytic domain of TET1 in mammalian cells

Depositor

Jean-Pierre Issa

Article

Jin et al Nucleic Acids Res. 2014 Jun;42(11):6956-

Insert

Tet Methylcytosine Dioxygenase 1 (TET1 Human)

Use

Tags

3X FLAG tag and hr GFP II

Expression

Mammalian

Mutation

TET1 amino acids 1-1417 deleted, catalytic domain…

Promoter

CMV

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pirespuro2-CTLA4-mEos2

Plasmid

#98285

Purpose

mammalian expression of CTLA4-mEos2

Depositor

Mike Heilemann

Article

Fricke et al Sci Rep. 2015 Sep 11;5:14072. doi: 10.10

Insert

CTLA4 (CTLA4 Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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TRF2-IRESS-eGFP

Plasmid

#19798

Depositor

Mark Mattson

Article

van Steensel et al Cell. 1998 Feb 6. 92(3):401-13.

Insert

TRF2 (TERF2 Human)

Use

Tags

Expression

Mammalian

Mutation

When compared to GenBank reference sequence NP_00…

Promoter

CMV-F

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
pIRES2-smGFP-Kv2.1

Plasmid

#131709

Purpose

Expresses Kv2.1 in mammalian cells

Depositor

Jennifer Kearney

Article

Kang et al Ann Neurol. 2019 Dec;86(6):899-912. doi:

Insert

KCNB1 (KCNB1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pINO4-Pfv-Sapphire-HO

Plasmid

#203659

Purpose

Yeast vector encoding donor DNA for integration of 40nm GEMs into the HO locus.

Depositor

Liam Holt

Article

Shu T et et al bioRxiv 2022

Insert

PfV

Use

CRISPR

Tags

Sapphire

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-mtagBFP2-IRESMuro

Plasmid

#82335

Purpose

Vector targeting the mtagBFP2 gene to the GAPDH locus of human cells. BFP is expressed from a 2A sequence at the C terminus of GAPDH. Puromycin resistance is encoded by the Muro gene.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

mtagBFP2-IRESMuro

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only

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DD-DamV133A-IRES2-mCherry

Plasmid

#159600

Purpose

Shield-1 inducible transient mammalian expression of Dam alone for DamID controls, containing the V133A mutant of Dam.

Depositor

Aaron Streets

Article

Altemose et al Cell Syst. 2020 Oct 21;11(4):354-366.e9.

Insert

DD-DamV133A-IRES2-mCherry

Use

Tags

Expression

Mammalian

Mutation

Dam V133A mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-eGFP

Plasmid

#164519

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible GFP and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLPCX-IRES-GFP

Plasmid

#65436

Purpose

For transfection or retroviral production. Mammalian expression of EGFP driven by the ECMV IRES.

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

IRES-GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

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