We narrowed to 17,815 results for: Por
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EGFP-uTEV1-MAVS-mTA4
Plasmid#171002PurposeHiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[Y536delinsFIVLI]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC39A14
Plasmid#132105PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC39A14 (SLC39A14 Human)
ExpressionMammalianAvailable SinceDec. 3, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
Hy_pMT Flag-Hel25E K91N
Plasmid#110121PurposeExpresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutationK91N mutationPromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT Flag-Hel25E D193E
Plasmid#110120PurposeExpresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinityDepositorInsertHel25E (Hel25E Fly)
TagsFlagExpressionInsectMutationD193E mutationPromoterMetallothionein Promoter (pMT)Available SinceSept. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR221_SLC7A3
Plasmid#132301PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC7A3 (SLC7A3 Human)
ExpressionMammalianAvailable SinceNov. 4, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
FL MALT1 Catalytic Mutant in pET29b-Strep
Plasmid#48969PurposeExpresses Full length catalytic mutant MALT1 in e.coliDepositorInsertMALT1 isoform A (MALT1 Human)
TagsHis and StrepExpressionBacterialMutationCatalytic Mutant C464APromoterT7Available SinceNov. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-Crry-T2A-GFP
Plasmid#205452Purposelentiviral plasmid for expression of mouse CrryDepositorAvailable SinceOct. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_32B
Plasmid#75011PurposePortion of Zika Virus genome (KU312312: 7066-7399) containing sensor 32B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_32B
ExpressionBacterialPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
ZIKV_NASBA_27B
Plasmid#75010PurposePortion of Zika Virus genome (KU312312: 1248-1594) containing sensor 27B trigger sequence and NASBA amplification sitesDepositorInsertZIKV_NASBA_27B
ExpressionBacterialPromoterT7Available SinceApril 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBa.GFP-KIF17 tail 400-1038
Plasmid#134617PurposeMammalian expression of mouse KIF1A 400-1038DepositorInsertKIF17 (Kif17 Mouse)
TagseGFPExpressionMammalianMutationWT with deletion of the 399 N-terminal residues.PromoterChicken Beta actinAvailable SinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-F-API5
Plasmid#157662PurposeExpresses API5 in mammalian cellDepositorAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-GSDMD_NoCys-Myc
Plasmid#201169PurposeExpression of GSDMD gene in mammalian cells by retroviral transductionDepositorInsertGasdermin D (Gsdmd Mouse)
UseRetroviralTagsMycMutationC39A, C57A, C77A, C122A, C265A, C192APromoterMESVAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCS2 Notch1 LNG-6MT
Plasmid#41738DepositorInsertNotch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
Tags6xMycExpressionMammalianMutationContains murine Notch-1 leader peptide (aa 1-23) …Available SinceJan. 14, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28b-API5
Plasmid#157655PurposeExpresses API5 in E.coli cellDepositorAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-Puro HLAG-2B-sgRNA
Plasmid#182552PurposeCas9 from S.pyogenes with 2A-Puro, and the 2B-sgRNA to targeting exon 2 of human HLA-G geneDepositorInserthSpCas9-2A-Puro-HLAG-2B-sgRNA
UseCRISPRExpressionMammalianPromoterCbhAvailable SinceMay 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IP/SECFP-mATG5
Plasmid#58744PurposeExpresses /SECFP-ATG5 in mammalian cellsDepositorAvailable SinceAug. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
p.UTA.2.0 miR-27a-3p 3 perfect copies.
Plasmid#82439PurposeDual Fluorescent endogenous miRNA sensor. Perfect complementary region of hsa-miR-27a-3p in three copiesDepositorInserthsa-miR-27a-3p (MIR27A Human)
MutationThree copies hsa-miR-27a-3p Complementary region …Available SinceMarch 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT2EFCRET(#324)
Plasmid#184059Purposecyclofen-inducible CRE activation in zebrafish transient transgenicDepositorInsertCRE-ERT2
UseZebrafish transient transgenesisMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterXl-EF1aAvailable SinceJuly 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPN098
Plasmid#91625PurposeExpress sgRNA targeting human HCN1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN099
Plasmid#91626PurposeExpress sgRNA targeting human HCN1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_nat
Plasmid#184914PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable SinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-JNKsub(TA)DD-YPet-NES
Plasmid#84634PurposeEncodes negative-control C-terminal (substrate) fragment of bimolecular JNK activity reporter (bimJNKAR); cytosol targeted; use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertJNKsub(TA)DD-YPet-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD8a-f-CTS-EGFP
Plasmid#86063PurposeA mammalian expression plasmid encoding fibrocystin mimentic clone CD8a-f-CTS (CD8a luminal and transmembrane domains, fibrocystin ciliary targeting signal domains) with C-terminus EGFP.DepositorInsertFibrocystin (Pkhd1 Mouse)
TagsGFPExpressionMammalianMutationcontains CD8a luminal and transmembrane domains a…PromoterCMVAvailable SinceMay 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG470 LifeAct-iFAST
Plasmid#130821PurposeExpresses LifeAct-iFAST in mammalian cells (actin labeling)DepositorInsertLifeAct-iFAST
Tagsmyc-tagExpressionMammalianPromoterCMVAvailable SinceSept. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEST-p70a-UTR1-Nterm 6xHis PL-T500
Plasmid#92314PurposeExpression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1DepositorInsertB1 domain of protein L (PL)
UseSynthetic Biology; Cell free protein synthesis (c…Tags6xHisExpressionBacterialPromoterp70aAvailable SinceOct. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21 Catalytic Domain of MALT1 Catalytic Mutant
Plasmid#48971PurposeExpresses catalytic domain of MALT1 catalytic mutant in e.coli AA 329-566DepositorInsertMALT1 (MALT1 Human)
TagsHisExpressionBacterialMutationContains AA 329-566; Catalytic Mutant C464APromoterT7Available SinceOct. 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG498 MAP4-iFAST
Plasmid#130820PurposeExpresses MAP4-iFAST in mammalian cells (microtubule labeling)DepositorInsertMAP4-iFAST
TagsMyc-tagExpressionMammalianPromoterCMVAvailable SinceOct. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
GFP-Tac-TC
Plasmid#162492PurposeExpresses partial length Tac (TMD and cytosolic tail) with GFP tag in mammalian cellsDepositorInsertpartial length Tac (TMD and cytosolic tail) (IL2RA Human)
TagsGFPExpressionMammalianMutationTac is in partial length with its TMD and cysotol…Available SinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCI-TPI_WT-xrRNA-4MS2-4H
Plasmid#108370PurposeExpresses wild type TPI reporter; enables detection of 5'-3' decay intermediates (xrFrag); 4MS2 tethering sites downstream of xrRNA and allows detection via northern blot (4H binding sites)DepositorInsertWild type TPI reporter with 4MS2 binding sites downstream of MVE xrRNA and 4H probe binding sites (TPI1 Human)
ExpressionMammalianPromoterCMVAvailable SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
API5-CRISPR
Plasmid#157664PurposeExpresses Cas9 and sgRNA targeting API5DepositorAvailable SinceAug. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-MAVS-mTA
Plasmid#170999PurposeHiLITR protease with MAVS [R537A] C-terminal tmd targeting information (Mitochondria and ER insertion)DepositorInsertEGFP-uTEV1-MAVS(tmd)[R537A]
UseLentiviral and Synthetic BiologyExpressionMammalianMutationMAVS(tmd)[R537A]PromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNIA-CEN-Venus-FLAG-LINXa4-Bre1
Plasmid#73618PurposeVisualizing the nuclear translocation of LINXa4-Bre1 in yeast.DepositorInsertVenus
UseSynthetic BiologyTagsFLAGExpressionYeastMutationBre1 point mutations K8A, K9A and K11APromoterADH1Available SinceApril 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPN235
Plasmid#91592PurposeExpress sgRNA targeting human CYFIP1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
FKBP-GTSE1(638-720)-GFP
Plasmid#100735PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosisDepositorInsertGTSE1 (GTSE1 Human)
TagsFKBP and GFPExpressionMammalianMutationC-terminus onlyPromoterCMVAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN234
Plasmid#91591PurposeExpress sgRNA targeting human CYFIP1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PKCsub(T/A)-YPet-NES
Plasmid#138208PurposeEncodes negative-control C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NESDepositorInsertPKCsub(T/A)-YPet-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceSept. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A dn-cHSF1
Plasmid#134724PurposeGateway entry vector for constitutively active dominant-negative HSF1DepositorInsertdn-cHSF1 (HSF1 Human)
UseGateway CloningMutationDeletion of amino acids 186-202, 379−529PromoterNoneAvailable SinceJuly 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPN262
Plasmid#91607PurposeExpress sgRNA targeting human EPHX2DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN107
Plasmid#91636PurposeExpress sgRNA targeting human KDM4ADepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only
