We narrowed to 31,552 results for: Eng;

1. pBRC1

   Plasmid

   #183064

   Purpose

   Base editing plasmid for Pseudomonas chlororaphis

   Depositor

   Xuehong Zhang

   Article

   Yue et al Microb Biotechnol. 2022 Sep;15(9):2324-2

   Insert

   eSpCas9ppD10A
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pNBU2_erm_P1T_DP-A21

   Plasmid

   #90318

   Purpose

   Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS A21

   Depositor

   Andrew Goodman

   Article

   Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAL-pyk_NT

   Plasmid

   #74072

   Purpose

   pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR

   Depositor

   Timothy Lu

   Article

   Cleto et al ACS Synth Biol. 2016 Feb 16.

   Insert

   sgRNA pyk (NT)
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ptac

   Available since

   April 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Cas9m2

   Plasmid

   #47314

   Purpose

   Cas9 D10A+H840A

   Depositor

   George Church

   Article

   Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

   Insert

   Cas9m2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pET21a(+)-Histag-Citrine (525)

   Plasmid

   #70718

   Purpose

   Expression vector for Citrine purification

   Depositor

   Richard Murray

   Article

   Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

   Insert

   Citrine
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDA040.BLD7-mRFP

   Plasmid

   #206800

   Purpose

   EL222_D7_mRFP

   Depositor

   James Carothers

   Article

   Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

   Insert

   BLD7.mRFP
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGT-S2

   Plasmid

   #122583

   Purpose

   MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

   Depositor

   Harris Wang

   Article

   Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

   Insert

   beta-lactamase/sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJPC12-ΔM13-mCherry-P/PM,5G6G-GFP

   Plasmid

   #80911

   Purpose

   Reporter plasmid (mCherry and GFP)

   Depositor

   Mark Isalan

   Article

   Brodel et al Nat Commun. 2016 Dec 16;7:13858. doi: 10

   Inserts

   mCherry
   GFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P5G6G and PM,5G6G

   Available since

   Dec. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. InterCatch-GFP

   Plasmid

   #186900

   Purpose

   Cell-free expression of transmembrane protein TfR-sfGFP-SpyCatcher-sfCherry1-10

   Depositor

   Allen Liu, Taekjip Ha

   Article

   Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

   Insert

   TfR-sfGFP-SpyCatcher003-sfCherry1-10
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p2301y-tdtomato-dCas9_DegronDead_TPLN188

    Plasmid

    #205409

    Purpose

    dCas9 guided repressor

    Depositor

    Jennifer Nemhauser

    Article

    Yang et al Quant Plant Biol. 2023 Oct 19;4:e12. doi

    Insert

    dCas9_DegronDead_TPLN188
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSEVA83-GG

    Plasmid

    #208016

    Purpose

    for cloning homology arms, with GFP as counter selection marker

    Depositor

    Pablo Ivan Nikel

    Article

    Lammens et al Microbiol Spectr. 2023 Nov 17:e0270723.

    Insert

    msfGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pBS Wnt-1HA

    Plasmid

    #17367

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-1HA (Wnt1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

13. p8xEGFP-N1

    Plasmid

    #122168

    Purpose

    Expression of 8xEGFP

    Depositor

    Georg Mayr

    Article

    Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

    Insert

    8xEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PSD95-TS:YSOG2

    Plasmid

    #42227

    Depositor

    Roger Tsien

    Article

    Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

    Insert

    PSD95
    

    Use

    Tags

    TS:YSOG2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pKmK.I2R

    Plasmid

    #125064

    Purpose

    Storage plasmid for K.marxianus right homology arm

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Right homology arm for targeting integration to chromosome V: 23743..21744
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p12xEGFP-N1

    Plasmid

    #122172

    Purpose

    Expression of 12xEGFP

    Depositor

    Georg Mayr

    Article

    Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

    Insert

    12xEGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLNC Wnt-7bHA

    Plasmid

    #18037

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-7b (Wnt7b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

18. pMT Draper-mCherry

    Plasmid

    #126667

    Purpose

    Inducible expression of Draper-mCherry in S2 cells

    Depositor

    Ron Vale

    Article

    Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

    Insert

    Draper mCherry
    

    Use

    Tags

    Expression

    Insect

    Mutation

    C813Y- see depositor comments below

    Promoter

    Available since

    June 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGD431

    Plasmid

    #132382

    Purpose

    contains the sequence of mRuby3for C-term fusions in the pGGD000 backbone (GreenGate)

    Depositor

    Rüdiger Simon

    Article

    Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

    Insert

    mRuby3
    

    Use

    Greengate entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAL-pgi_T

    Plasmid

    #74066

    Purpose

    pAL374 plasmid carrying the pgi (T) sgRNA, targeting the template strand of pgi, SpecR

    Depositor

    Timothy Lu

    Article

    Cleto et al ACS Synth Biol. 2016 Feb 16.

    Insert

    sgRNA pgi (T)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ptac

    Available since

    April 12, 2016

    Availability

    Academic Institutions and Nonprofits only

21. pCfB8739

    Plasmid

    #126905

    Purpose

    Template for PCR amplification of XdCrtYB

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtYB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. HEK3_pegF_60bp

    Plasmid

    #174856

    Purpose

    Express forward pegRNA mediating 991-bp deletion and 60-bp insertion

    Depositor

    Wen Xue

    Article

    Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    HEK3_pegF_60bp insertion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. HEK3_pegR_60bp

    Plasmid

    #174857

    Purpose

    Express reverse pegRNA mediating 991-bp deletion and 60-bp insertion

    Depositor

    Wen Xue

    Article

    Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

    Insert

    HEK3_pegR_60bp insertion
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGT-B2

    Plasmid

    #122580

    Purpose

    MAGIC donor plasmid (pBBR1 oriR with beta-lactamase/sfGFP payload)

    Depositor

    Harris Wang

    Article

    Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pDUET-aaCPRct-A13AMO

    Plasmid

    #20116

    Depositor

    Jay Keasling

    Article

    Chang et al Nat Chem Biol. 2007 May . 3(5):274-7.

    Insert

    p450 reductase and Amorphadiene oxidase
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

26. pGD250

    Plasmid

    #132371

    Purpose

    contains the sequence of mClover3 for C-term fusions in the pGGD000 backbone (GreenGate)

    Depositor

    Rüdiger Simon

    Article

    Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

    Insert

    linker-mClover3
    

    Use

    Greengate entry plasmid

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNC-KSE401

    Plasmid

    #193435

    Purpose

    Nimble cloning-compatible vector for genome editing in plants

    Depositor

    Pu Yan

    Article

    Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. promoter-less mRFP1E

    Plasmid

    #183144

    Purpose

    BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E

    Depositor

    Anthony Forster

    Article

    Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

    Insert

    RBS, mRFP1E
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    BioBrick sites removed

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLNC Wnt-4HA

    Plasmid

    #18031

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-4HA (Wnt4 Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

30. pBS Wnt-4HA

    Plasmid

    #17375

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-4HA (Wnt4 Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

31. pBS Wnt-5aHA

    Plasmid

    #17376

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-5aHA (Wnt5a Mouse)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

32. pND004

    Plasmid

    #200950

    Purpose

    MBP-tagged E. coli protein expression destination vector for Golden Gate cloning

    Depositor

    Timothy Whitehead

    Article

    Daffern et al Biotechnol Bioeng. 2024 Jan;121(1):281-2

    Type

    Empty backbone

    Use

    Tags

    MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pENTR1a-jAspSnFR3-mRuby3

    Plasmid

    #203491

    Purpose

    jAspSnFR3-mRuby3 in Gateway cloning entry vector

    Depositor

    Lucas Sullivan

    Article

    Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

    Insert

    jAspSnFR3-mRuby3
    

    Use

    Gateway entry vector

    Tags

    His-tag and mRuby3

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pYUB28b-MCS-optimized

    Plasmid

    #89179

    Purpose

    mycobacteria shuttle vector - pYUB28b with a single change to the MCS

    Depositor

    Ted Baker, Ghader Bashiri

    Article

    Bashiri et al PLoS One. 2010 Dec 29;5(12):e15803.

    Type

    Empty backbone

    Use

    Mycobacteria shuttle vector

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNBU2_erm_P1T_DP-B41

    Plasmid

    #90321

    Purpose

    Integration vector at an attB site in Bacteroides; contains the P1T_DP aTC-inducible promoter with the RBS B41

    Depositor

    Andrew Goodman

    Article

    Lim et al Cell. 2017 Apr 20;169(3):547-558.e15. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pKmK.I4R

    Plasmid

    #125066

    Purpose

    Storage plasmid for K.marxianus right homology arm

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    Right homology arm for targeting integration to chromosome IV:240042..241741
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCfB8740

    Plasmid

    #126906

    Purpose

    Template for PCR amplification of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCfB8741

    Plasmid

    #126907

    Purpose

    Template for PCR amplification of XdCrtE

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLNC Wnt-3HA

    Plasmid

    #18029

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-3 (WNT3 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    Available since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

40. pUC19-THG73tRNA

    Plasmid

    #21292

    Depositor

    Henry Lester

    Article

    Saks et al J Biol Chem. 1996 Sep 20. 271(38):23169-

    Insert

    T. thermophila tRNA Glutamine (CUA)-G73
    

    Use

    T. thermophila

    Tags

    Expression

    Mutation

    N/A

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge