We narrowed to 27,270 results for: Tat

1. Bov.hsc70.RSET.a1-554.wt(NarI)

   Plasmid

   #12533

   Depositor

   David McKay

   Article

   Ha et al J Biol Chem. 1997 Oct 31. 272(44):27796-

   Insert

   Bovine 70 kDa heat shock cognate protein fragment amino acids 1-554
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   deleted carboxy-terminal residues 555-650 of bovi…

   Promoter

   Available since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEF Doc2alpha D291N mutant GFP

   Plasmid

   #128821

   Purpose

   To visualize Doc2alpha D291N mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2alpha (Doc2a Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D291N

   Promoter

   EF-1alpha

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEF Doc2beta D303N mutant GFP

   Plasmid

   #128822

   Purpose

   To visualize Doc2beta D303N mutant in PC12 cells

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Doc2beta (Doc2b Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D303N

   Promoter

   EF-1alpha

   Available since

   Nov. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRRG319-nlk

   Plasmid

   #99082

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea nemo-like kinase (also known as h.109.4e)

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

   Insert

   nemo-like kinase (also known as h.109.4e) in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-ERBB4 (E563K)

   Plasmid

   #29532

   Depositor

   Yardena Samuels

   Article

   Prickett et al Nat Genet. 2009 Oct . 41(10):1127-32.

   Insert

   ERBB4 (ERBB4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   changed glutamic acid 563 to lysine

   Promoter

   Available since

   Sept. 7, 2011

   Availability

   Academic Institutions and Nonprofits only

6. pENTR-TER+-shLKB1-Ms

   Plasmid

   #61234

   Purpose

   Entry clone encoding shRNA to mouse LKB1

   Depositor

   Reuben Shaw

   Article

   Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

   Insert

   shLkb1 (Ms/Hu) (STK11, Stk11 Human, Mouse)
   

   Use

   Entr clone

   Tags

   Expression

   Mutation

   Promoter

   H1/TO

   Available since

   Jan. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pDONR221-hSOX10-Val15AlafsX11

   Plasmid

   #24732

   Depositor

   William Pavan

   Article

   Cronin et al Pigment Cell Melanoma Res. 2009 Aug . 22

   Insert

   (sex determining region Y)-box 10 (SOX10 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   deleted amino acids 44-62. deletion/frameshift…

   Promoter

   Available since

   June 1, 2010

   Availability

   Academic Institutions and Nonprofits only

8. ACTL6A C8.3 gRNA

   Plasmid

   #90521

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human ACTL6A

   Depositor

   Iain Cheeseman

   Article

   McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

   Insert

   ACTL6A (Guide Designation C8.3)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   Aug. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pECE M2-SNX17 S437A

   Plasmid

   #69812

   Purpose

   Expresses M2-SNX17 in mammalian cells

   Depositor

   Anne Brunet

   Article

   Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

   Insert

   Sorting nexin-17 (SNX17 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   SV40 early promoter

   Available since

   May 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAP2

    Plasmid

    #41755

    Depositor

    Jeff Gelles

    Article

    Martin et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    kinesin heavy chain (Khc E. coli, Fly)
    

    Use

    Tags

    His6 and bccp

    Expression

    Bacterial

    Mutation

    residues 1-401; C45S C338S K349C C451S

    Promoter

    Available since

    July 29, 2013

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-myc-Sema4DdeltaC

    Plasmid

    #51602

    Purpose

    expresses Sema4D lacking C terminus

    Depositor

    Suzanne Paradis

    Article

    Raissi et al Mol Cell Neurosci. 2013 Nov;57:23-32. do

    Insert

    Sema4DdeltaC (Sema4d Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Last 69 amino acids of Sema4D C-terminus are dele…

    Promoter

    CMV

    Available since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3.1(+)_UG3

    Plasmid

    #133874

    Purpose

    Expresses UG3 in mammalian cells.

    Depositor

    Sumio Terada

    Article

    Nakai et al Microscopy (Oxf). 2019 Jul 2. pii: 55252

    Insert

    UtrCH (UTRN Human)
    

    Use

    Tags

    helical linker - mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLIK-Venus-TmiR-G13

    Plasmid

    #25741

    Purpose

    Lentiviral vector with Tet-based inducible expression of mouse G alpha 13 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. ROZA-XL YF

    Plasmid

    #64195

    Purpose

    Fluorescent reporter for ZAP-70 tyrosine kinase activity- Y to F mutated control sequence

    Depositor

    Annemarie Lellouch

    Article

    Cadra et al Biochem Biophys Res Commun. 2015 Feb 28.

    Insert

    ROZA-XL YF
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    ROZA-XL tyrosine phosphorylation site mutated to …

    Promoter

    CMV

    Available since

    April 23, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pRSET-pr-mEos4a (mEosFP4a-V69T)

    Plasmid

    #99225

    Purpose

    Bacterial expression of the primed conversion capable protein pr-mEos4a (mEos4a-V69T).

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

    Insert

    pr-mEos4a
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V69T for primed conversion applications

    Promoter

    T7

    Available since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Cerulean(1-158) in pcDNAI/Amp

    Plasmid

    #60897

    Purpose

    This vector attaches Cerulean(1-158) to the N-terminus of a protein.

    Depositor

    Catherine Berlot

    Article

    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E

    Insert

    Cerulean(1-158)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-EF1α-FRT-FLEX-GtACR2-EYFP-TlcnC

    Plasmid

    #114374

    Purpose

    expresses Flpo recombinase-dependent GtACR2-TlcnC, which targets GtACR2 to the somatodendritic compartment

    Depositor

    Mingshan Xue

    Article

    Messier et al Elife. 2018 Aug 9;7. pii: 38506. doi: 10

    Insert

    GtACR2-EYFP-TlcnC (NEWENTRY )
    

    Use

    AAV

    Tags

    EYFP and TlcnC

    Expression

    Mammalian

    Mutation

    Promoter

    Ef1a

    Available since

    Aug. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. XE125 XDsh delta COOH-GFP-CS2+

    Plasmid

    #16784

    Depositor

    Randall Moon

    Article

    Miller et al J Cell Biol. 1999 Jul 26;146(2):427-37.

    Insert

    Xdsh-delta-COOH (dvl2.L Frog)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    contains Dsh amino acids 1-588 (deleting 599-736)

    Promoter

    Available since

    March 24, 2008

    Availability

    Academic Institutions and Nonprofits only

19. FgH1tUTG_huMcl-1.2

    Plasmid

    #85531

    Purpose

    Inducible expression of guide RNA (huMcl-1.2) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Mcl-1.2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR3-vsv-CenpB 1-166-Sgo1 N61I

    Plasmid

    #108498

    Purpose

    expression of vsv-CenpB 1-166-Sgo1 N61I (no PP2A binding)

    Depositor

    Susanne Lens

    Article

    Meppelink et al Cell Rep. 2015 Apr 28;11(4):508-15. doi:

    Insert

    CenpB-Shugoshin 1 (SGO1 Human)
    

    Use

    Tags

    VSV

    Expression

    Mammalian

    Mutation

    CenpB 1-166 fusion before Sgo1, no PP2A binding N…

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. SGO1 H2.1 gRNA

    Plasmid

    #90887

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human SGO1

    Depositor

    Iain Cheeseman

    Article

    McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

    Insert

    SGO1 (Guide Designation H2.1)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    July 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGEX-4T1-Claspin-224N

    Plasmid

    #31634

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    1st 224 amino acids of claspin

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

23. pEGFP-STAG2-K225insNM

    Plasmid

    #31974

    Depositor

    Todd Waldman

    Article

    Solomon et al Science. 2011 Aug 19;333(6045):1039-43.

    Insert

    human STAG2 cDNA (STAG2 Human)
    

    Use

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Mutation

    inserted amino acids Asn-Met before Lys225

    Promoter

    Available since

    Aug. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

24. pPD95.75-cYAP-1 S104A

    Plasmid

    #66858

    Purpose

    Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

    Insert

    YAP-1 (yap-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Worm

    Mutation

    Serine104 is mutated to alanine

    Promoter

    Available since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

25. pJK597

    Plasmid

    #71121

    Purpose

    Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

    Depositor

    T. J. Kappock

    Article

    Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

    Insert

    formyl-CoA:oxalate CoA-transferase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. MSCV-PM BRD7 sh3

    Plasmid

    #41930

    Depositor

    Stephen Elledge

    Article

    Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

    Insert

    BRD7 (BRD7 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 10, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pEYFP-N1-hRIP3-FL VV458/460PP

    Plasmid

    #61368

    Purpose

    Expresses Human full length RIPK3 with A458P/V460P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP3 with VV458/460P mutation (RIPK3 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed V458 to P and V460to P

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

28. pEGFP-N1-hRIP1-FL I539P

    Plasmid

    #61380

    Purpose

    Expresses Human full length RIPK1with I539P mutation in the mammalian cells

    Depositor

    Francis Chan

    Article

    Li et al Cell. 2012 Jul 20;150(2):339-50. doi: 10

    Insert

    Full length human RIP1with I539P mutation (RIPK1 Human)
    

    Use

    Tags

    EGFP and HA tag

    Expression

    Mammalian

    Mutation

    changed I539 to P

    Promoter

    Available since

    Jan. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pBABEpuro-ARAF_S214F

    Plasmid

    #51078

    Purpose

    mammalian expression of ARAF S214F

    Depositor

    Matthew Meyerson

    Article

    Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

    Insert

    ARAF (ARAF Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    S214F

    Promoter

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5/FRT/TO HIS DNAJB7

    Plasmid

    #19557

    Depositor

    Harm Kampinga

    Article

    Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

    Insert

    DnaJ (Hsp40) homolog, subfamily B, member 7 (DNAJB7 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 6, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pGEX-4T1-Claspin-BPI

    Plasmid

    #31633

    Depositor

    Aziz Sancar

    Article

    Lindsey-Boltz et al J Biol Chem. 2009 Nov 27. 284(48):33107-

    Insert

    Claspin (CLSPN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 273-492 of claspin

    Promoter

    Available since

    Nov. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

32. pcDNA3.2/V5 hsa-mir-220a

    Plasmid

    #26314

    Depositor

    David Bartel

    Article

    Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

    Insert

    hsa-mir-220a (MIR220A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2010

    Availability

    Academic Institutions and Nonprofits only

33. GFP-Nsp3(1-111)

    Plasmid

    #242947

    Purpose

    GFP and the Ubl1 domain of the SARS-CoV-2 Nsp3 protein (aa 1-111)

    Depositor

    Benjamin Schuster

    Article

    Favetta et al bioRxiv [Preprint]. 2025 Jun 10:2024.10.

    Insert

    GFP-Nsp3(1-111) (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pETDuet-solo-NHis6-NocO-D282A

    Plasmid

    #240407

    Purpose

    expresses N-His tagged NocO D282A (diiron halogenase variant) from Nodularia sp. 06071 (mutation numbering based on amino acid position in native sequence), created from mutagenesis of WT plasmid

    Depositor

    Emily Balskus

    Article

    Wang et al Biochemistry. 2025 May 20;64(10):2173-21

    Insert

    nocO
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    M1 of native sequence not included in insert, Asp…

    Promoter

    T7

    Available since

    Aug. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. ABE8e SpG Puro

    Plasmid

    #235044

    Purpose

    Adenosine base editor 8e* with SpG nicking Cas9 makes A > G edits

    Depositor

    Justin Pritchard

    Article

    Sokirniy et al Nucleic Acids Res. 2025 Jul 19;53(14):gk

    Insert

    ABE8e, nCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    P2A-PuroR

    Expression

    Mutation

    Promoter

    EF1a core

    Available since

    Aug. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pMT-Myc-BirA*G3

    Plasmid

    #240233

    Purpose

    Expression of Myc-BirA*-G3 under control of copper-inducible promoter. Can be used to generate stable cell lines by Hygromycin selection.

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    Myc-BirA*G3
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    MT

    Available since

    Aug. 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-p53-G609T

    Plasmid

    #229531

    Purpose

    Expresses p53 mutant CASM203 in mamallian cells

    Depositor

    Robin Fåhraeus

    Article

    Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

    Insert

    p53-CDS (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.G609T

    Promoter

    CMV

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3-p53-G609A

    Plasmid

    #229532

    Purpose

    Expresses p53 mutant G609A in mamallian cells

    Depositor

    Robin Fåhraeus

    Article

    Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

    Insert

    p53-CDS (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.G609A

    Promoter

    CMV

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3-p53-G609C

    Plasmid

    #229533

    Purpose

    Expresses p53 mutant G609C in mamallian cells

    Depositor

    Robin Fåhraeus

    Article

    Sajwan et al Br J Cancer. 2025 Jul 26. doi: 10.1038/s

    Insert

    p53-CDS (TP53 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    c.G609C

    Promoter

    CMV

    Available since

    July 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pENTR_CG6867_nostop

    Plasmid

    #240238

    Purpose

    Gateway entry clone with CG6867 without stop codon

    Depositor

    Justin Bosch, Norbert Perrimon

    Article

    Bosch et al bioRxiv 2025.07.09.659702; doi: https://

    Insert

    CG6867 (CG6867 Fly)
    

    Use

    Gateway shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge