We narrowed to 30,684 results for: PLE;
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pCfB2797
Plasmid#63639PurposepCfB2797 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
p405-BrdU-Inc
Plasmid#71791PurposeBrdU-incorporating plasmid with selectable marker LEU2DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
ShadowG-SUMO1
Plasmid#175239PurposeBacterial expression and purification, conjugatable SUMO for SUMOylationDepositorAvailable SinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMD19-PBBa_J23117-sgRNA-Spr
Plasmid#190793PurposeTemplate vector to amplify single sgRNAs or pieces for multiplexing arrays. Has flanking BsaI-sites.DepositorInsertsgRNA (dummy)
UseSynthetic BiologyExpressionBacterialPromoterBBa_J23117Available SinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
p404-BrdU-Inc
Plasmid#71790PurposeBrdU-incorporating plasmid with selectable marker TRP1DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMPE-ePPEplus
Plasmid#205242PurposeFor multiplex plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertCsy4-c-myc NLS-bpNLS-nCas9-NC-MLV-vbpNLS-SV40NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCfB2800
Plasmid#63657PurposepCfB2800 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
iMAP-61
Plasmid#187460PurposegRNA array of iMAP-61DepositorInsertsgRNA array
UsePiggybacExpressionMammalianPromotermodified U6Available SinceOct. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBA409
Plasmid#85971PurposeEF1a-sfGFP-Ubc-NeoDepositorInsertssfGFP
Neo
UseLentiviralExpressionMammalianAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ10704
Plasmid#183957PurposeU6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFPDepositorInsertSKSKSO array
UseLentiviralTagsEF1a-Puro-T2A-TagBFPExpressionMammalianPromoterU6Available SinceAug. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMD19T-psba1-TetR-PL22-dCas9-SpR
Plasmid#73223PurposeContains dCas9 from S. pyogenes under aTc inducible promoter. Suicide vector inserts into psba1 site of Synechocystis. Carries spectinomycin resist. Recommend E. coli Copy cutter for propogation.DepositorInsertdCas9 from S. pyogenes
Tagsc-mycExpressionBacterialMutationSilent mutation at bp 1341 A->C to remove an E…PromoterPL22Available SinceMarch 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2793
Plasmid#64043PurposepCfB2793 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.URA3. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoter and noneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBA407
Plasmid#85970PurposeUPRE-mCh-Ubc-NeoDepositorInsertsmCherry
Neo
UseLentiviralExpressionMammalianAvailable SinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK277 (mClover-NeoR)
Plasmid#72793PurposeC-terminal tagging with mClover using CRIPSR/Cas9DepositorInsertmClover-Neo
UseCRISPRTagsmCloverExpressionMammalianAvailable SinceMarch 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMRXIP-GFP-Evectin-2
Plasmid#221754PurposeStable expression of Evectin-2 in mammalian cellsDepositorInsertPLEKHB2 (PLEKHB2 Human)
UseRetroviralTagsGFPExpressionMammalianMutationtandem PLEKHB2(PH domain) (amino acids 1-110)Available SinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMK288 (mAID-Bsr)
Plasmid#72826PurposeC-terminal tagging with mAID using CRISPR/Cas9DepositorInsertmAID-Bsr
UseCRISPRTagsmAIDExpressionMammalianAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9
Plasmid#84246PurposeExpresses direct fusion VPR-Sa dCas9DepositorInsertsVPR-tagBFP-Sa dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTagsNucleoplasmin NLS, SV40 NLS, VPR, and tagBFPExpressionMammalianPromoterCAG and PGKAvailable SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE
Plasmid#140230PurposeDox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFPDepositorInsertLuciferase + crIFNg (reverse orientation)
UseLentiviralExpressionMammalianPromoterTRE3GAvailable SinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only
