We narrowed to 4,899 results for: phy

1. CBSH3- 3m shRNA2

   Plasmid

   #160211

   Purpose

   Knock Down MTLSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA2 targeting collybistin MTL-CBSH3-isoforms (Plasmid #160210)

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   TGACGTTGCTCaGGcTGtACCA (mutations shown in lower …

   Promoter

   mouse U6 promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. CBSH3- 3m shRNA1

   Plasmid

   #160209

   Purpose

   Knock Down MQWSH3- Collybistin isoforms, 3-point mutation negative control for CBSH3- shRNA1 targeting collybistin MQW-CBSH3- isoforms (Plasmid # 160208).

   Depositor

   Angel de Blas

   Article

   George et al J Neurochem. 2021 May;157(4):1032-1051.

   Insert

   ARHGEF9 (Arhgef9 Rat)
   

   Use

   RNAi

   Tags

   Expression

   Mutation

   GATCGGGAATGCTCaGGcTGtACC (mutations shown in lowe…

   Promoter

   mouse U6 promoter

   Available since

   Oct. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pABD823

   Plasmid

   #67911

   Purpose

   Protein interaction with yeast two hybrid assay of CTG10 in yeast, Saccharomyces cerevisiae

   Depositor

   Bruce Downie

   Article

   Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

   Insert

   Cold temperature germinating 10 (CTG10)
   

   Use

   Tags

   GAL4 DNA binding domain (148 amino acids)

   Expression

   Yeast

   Mutation

   Promoter

   pADH1 (alcohol dehydrogenase 1 promoter)

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

4. pABD933

   Plasmid

   #67917

   Purpose

   Entry clone made prior to studies of protein co-localization of CTG10 via fluorescent fusion after transient expression in planta

   Depositor

   Bruce Downie

   Article

   Majee et al Proc Natl Acad Sci U S A. 2018 Apr 24;11

   Insert

   CTG10
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

5. ORAI1-YFP V107M-L273D-L276D

   Plasmid

   #114183

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the constitutively activating mutation V107M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP V107M-L273D-L276D (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   V107M/L273D/L276D

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. ORAI1-YFP T184M-L273D-L276D

   Plasmid

   #114184

   Purpose

   ORAI1 channel with C-terminal YFP, carrying the activating mutation T184M from TAM, and the mutations L273D-L276D leading to a deficiency in STIM1-mediated gating.

   Depositor

   Nicolas Demaurex

   Article

   Bulla et al J Physiol. 2018 Nov 1. doi: 10.1113/JP27

   Insert

   ORAI1-YFP T184M-L273D-L276D (ORAI1 Human)
   

   Use

   Tags

   YFP

   Expression

   Mammalian

   Mutation

   T184M/L273D/L276D

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJC20cdc8.A18T

   Plasmid

   #99360

   Purpose

   Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Ala-18-Thr.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Alanine 18 to Threonine

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJC20cdc8.E31K

   Plasmid

   #99361

   Purpose

   Bacterial expression vector containing cDNA encoding for the fission yeast tropomyosin, Cdc8 with the amino acid substitution Glu-31-Lys.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Glutamic acid 31 to Lysine

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJC20cdc8.110

   Plasmid

   #99363

   Purpose

   Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.110.

   Depositor

   Dan Mulvihill

   Article

   Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

   Insert

   cdc8 (cdc8 Fission Yeast)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Alanine 18 to Threonine and Glutamic acid 31 to L…

   Promoter

   T7

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. ACA8-mYFP

    Plasmid

    #87245

    Purpose

    fluorescent labelling of ACA8

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    ACA8 (ACA8 Mustard Weed)
    

    Use

    Tags

    YFP

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. ACA8-YFPc

    Plasmid

    #87243

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    ACA8 (ACA8 Mustard Weed)
    

    Use

    Tags

    YFPc

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. ACA8-YFPn

    Plasmid

    #87241

    Purpose

    fluorescence complementation assay

    Depositor

    Silke Robatzek

    Article

    Frei dit Frey et al Plant Physiol. 2012 Jun;159(2):798-809.

    Insert

    ACA8 (ACA8 Mustard Weed)
    

    Use

    Tags

    YFPn

    Expression

    Mutation

    Promoter

    35s

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. attB-GFP-Dnmt3b6-Poly(A)-NeoR

    Plasmid

    #65552

    Purpose

    Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b6 cDNA into a MIN-tagged locus using Neomycin selection

    Depositor

    Sebastian Bultmann

    Article

    Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

    Insert

    Dnmt3b (Dnmt3b Mouse)
    

    Use

    Mouse Targeting; Bxb1

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    isoform 6

    Promoter

    Available since

    Aug. 31, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1-idCas9-KRAB-Hygro donor

    Plasmid

    #199621

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Insert

    AAVS1-idCas9-KRAB-Hygro
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-T7-ABE8e-nSpCas9-P2A-EGFP (KAC978)

    Plasmid

    #185910

    Purpose

    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    human codon optimized ABE8e-nSpCas9-P2A-EGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A

    Promoter

    CMV and T7

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pStreptagII 3C EGFR EABR

    Plasmid

    #234994

    Purpose

    For production of Extracellular Vesicles (EVs), with Epithelial Growth Factor Receptor on their surface, Strep-tag II labeled on its N-terminus

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    EGFR-EABR (EGFR Human)
    

    Use

    Tags

    Streptag-II, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHA StreptagII 3C NIS EABR

    Plasmid

    #234988

    Purpose

    For production of Extracellular Vesicles (EVs), with a Strep-tag II labeled Sodium-iodide symporter (NIS), and an HA epitope at its N-terminus on their surface

    Depositor

    Daniel Leahy, Monica Gonzalez-Magaldi

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    NIS-EABR (SLC5A5 Human)
    

    Use

    Tags

    HA, Streptag-II, HRV 3C site

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid

    #205703

    Purpose

    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. HDM_SARS2_Spike_del21_D614G

    Plasmid

    #158762

    Purpose

    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614

    Depositor

    Jesse Bloom

    Article

    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051

    Insert

    SARS2-Spike_del21_D614G (S Synthetic, Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted amino acids 1257-1278, changed aspartic a…

    Promoter

    CMV

    Available since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCC_08 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9NG-NLS-VPR-2A-Puro-WPRE

    Plasmid

    #139093

    Purpose

    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    dxCas9-NG-VPR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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