We narrowed to 24,606 results for: Nes

1. Antares-SEPLuc

   Plasmid

   #141086

   Purpose

   precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis

   Depositor

   Jeak Ling Ding

   Article

   Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

   Insert

   Antares-T2A-puromycin-EMCV IRES-SEPLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 66 to 91 on the Luciferas…

   Promoter

   CMV

   Available Since

   June 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pVenus-N1 cyclin B1 L45A (1324)

   Plasmid

   #61851

   Purpose

   mammalian expression of cyclin B1 L45 A mutant fused to Venus

   Depositor

   Jonathon Pines

   Article

   Matsusaka et al Biol Open. 2014 Sep 12;3(10):904-12. doi

   Insert

   cyclin B1 (CCNB1 Human)
   

   Use

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   L45A

   Promoter

   CMV

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pDGE76

   Plasmid

   #80578

   Purpose

   recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, nptII

   Depositor

   Johannes Stuttmann

   Article

   Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

   Inserts

   pnos:nptII-tmos
   p2x35S:Cas9 D10A-tnos
   BsaI-ccdB_CmR-BsaI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   D10A

   Promoter

   Available Since

   Sept. 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mCerulean3-H2B-C-10

   Plasmid

   #55422

   Purpose

   Localization: Nucleus/Histones, Excitation: 433, Emission: 475

   Depositor

   Michael Davidson

   Article

   Markwardt et al PLoS One. 2011 . 6(3):e17896.

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mCerulean3

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available Since

   Dec. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pUC 3GLA UAS HAi

   Plasmid

   #71763

   Purpose

   gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, Amp resistant, with 5xUAS and HA tag

   Depositor

   Matthias Soller

   Article

   Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pOT2 3GLA

   Plasmid

   #71764

   Purpose

   gap-repair recombineering for Drosophila transgenesis, contains loxP cassette with GFP, chloramphenicol resistant

   Depositor

   Matthias Soller

   Article

   Haussmann et al Nucleic Acids Res. 2018 Dec 12. pii: 523

   Type

   Empty backbone

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTAtUbq3 (GB0219)

   Plasmid

   #68192

   Purpose

   Provides the Arabidopsis thaliana polyubiquitin 3 terminator as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

   Insert

   AtUbq3 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available Since

   March 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLKO.1 shPIK3CD.v2 puro

   Plasmid

   #58707

   Purpose

   Lentiviral shRNA vector for knockdown of human PIK3CD

   Depositor

   Kevin Janes

   Article

   Bajikar et al Proc Natl Acad Sci U S A. 2014 Feb 4;111

   Insert

   shPIK3CD (PIK3CD Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pSTAP-seq_fly-tj

   Plasmid

   #86386

   Purpose

   Vector to measure enhancer responsiveness of candidates from a genomic library (cloned instead of the core promoter) by determining the abundance of transcripts originating from each candidate.

   Depositor

   Alexander Stark

   Article

   Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

   Insert

   D. melanogaster tj enhancer
   

   Use

   Stap-seq screening vector

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   June 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PGL3-Ogt (-178 to +233 bp)

    Plasmid

    #79266

    Purpose

    To identify the Cis and Trans acting elements that control Ogt gene transcription.

    Depositor

    Steven Jones

    Article

    Muthusamy et al J Biol Chem. 2015 Dec 25;290(52):31013-2

    Insert

    Ogt promoter element (Ogt Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

11. K349CLM-6His 215/347

    Plasmid

    #24451

    Depositor

    Ron Vale

    Article

    Tomishige et al Nat Struct Mol Biol. 2006 Oct . 13(10):8

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S, E215C, E347C

    Promoter

    Available Since

    July 15, 2010

    Availability

    Academic Institutions and Nonprofits only

12. A337C/Y344C

    Plasmid

    #24434

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, A337C/Y…

    Promoter

    Available Since

    June 14, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pDGE77

    Plasmid

    #80579

    Purpose

    recipient plasmid [multiplexing, nickase]; p35S:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    p2x35S:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pDGE78

    Plasmid

    #80580

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, nptII

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:nptII-tmos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDGE79

    Plasmid

    #80581

    Purpose

    recipient plasmid [multiplexing, nickase]; pPcUbi:Cas9 D10A, BASTA

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Inserts

    pnos:PAT-tnos
    pPcUbi:Cas9 D10A-tnos
    BsaI-ccdB_CmR-BsaI
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    D10A

    Promoter

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. K222C/E334C

    Plasmid

    #24435

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A, K222C/E…

    Promoter

    Available Since

    July 13, 2010

    Availability

    Academic Institutions and Nonprofits only

17. K420CLM RP

    Plasmid

    #24443

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S; aa 1-420 only

    Promoter

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pCINTBeta1

    Plasmid

    #14067

    Depositor

    Richard Hynes

    Article

    Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

    Insert

    Chicken Beta 1 Integrin (ITGB1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PstI-BglI fragment was blunt end cloned and EcoRI…

    Promoter

    Available Since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

19. CLM RP KGF Mut 1

    Plasmid

    #24166

    Depositor

    Ron Vale

    Article

    Tomishige et al J Cell Biol. 2000 Nov 27. 151(5):1081-92

    Insert

    Kinesin (KIF5B Human)
    

    Use

    Tags

    6XHis and GFP

    Expression

    Bacterial

    Mutation

    C7S/C65A/C168A/C174S/C294A/C330S/C421A

    Promoter

    Available Since

    May 5, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pX458-SLC35A1

    Plasmid

    #239952

    Purpose

    Encodes gRNA for human SLC35A1

    Depositor

    Jan Carette

    Article

    Varanese et al Nature. 2025 Mar 25. doi: 10.1038/s41586

    Insert

    SLC35A1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge