We narrowed to 11,216 results for: SHA

1. Lenti-guide-puro mYwhae - 1

   Plasmid

   #198497

   Purpose

   lentiviral stable expression of mYwhae gRNA 1

   Depositor

   Suzanne Pfeffer

   Article

   Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

   Insert

   mYwhae (Ywhae Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

   Plasmid

   #197958

   Purpose

   T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pcoCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   CmYLCVp and pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

   Plasmid

   #197963

   Purpose

   T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

   Depositor

   Steven Jacobsen

   Article

   Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

   Inserts

   pco-vCasphi-2
   AtPDS3 gRNA10
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26 and pUBQ10

   Available since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYI006

   Plasmid

   #170840

   Purpose

   FRO6p::GUSPlus::tOCS in pFP100

   Depositor

   Christian Fankhauser

   Article

   Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

   Insert

   FRO6p::GUSPlus::tOCS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   FRO6

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYI046

   Plasmid

   #170842

   Purpose

   (pUBQ10::SMT2-FLAG::tOCS)

   Depositor

   Christian Fankhauser

   Article

   Ince et al Nat Commun. 2022 Oct 10;13(1):5659. doi:

   Insert

   pUBQ10::SMT2-FLAG::tOCS
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   pUBQ10

   Available since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CBA-Con/Fon-EYFP-WPRE

   Plasmid

   #186752

   Purpose

   AAV-mediated Cre and Flp-dependent expression of EYFP under CBA promoter

   Depositor

   Yongling Zhu

   Article

   Xu et al Cell Rep. 2022 Jul 5;40(1):111036. doi:

   Insert

   EYFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CBA

   Available since

   July 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTL370

   Plasmid

   #69880

   Purpose

   attB flanked genomic Cirl for integration at attP sites.

   Depositor

   Tobias Langenhan, Robert Kittel

   Article

   Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

   Insert

   CIRL (Cirl Fly)
   

   Use

   Phic31-integration vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLPC-FLAG.HA.HA.Mm.RAP1.I312R

   Plasmid

   #184643

   Purpose

   Retroviral expressions of mouse RAP1 I312R

   Depositor

   Agnel Sfeir

   Article

   Barry et al Genes Dev. 2022 Feb 24. pii: gad.349039.

   Insert

   rap1
   

   Use

   Retroviral

   Tags

   HA FLAG

   Expression

   Mammalian

   Mutation

   I312R

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. 1056H

   Plasmid

   #183135

   Purpose

   Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

   Depositor

   Omar Akbari

   Article

   Kandul et al GEN Biotechnology (2022) 1:4, 372-385

   Insert

   U6.3-gRNAs[sxl, bTub]
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   June 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 1056J

    Plasmid

    #183136

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 1056K

    Plasmid

    #183137

    Purpose

    Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 1 gRNA targeting D.suzukii bTub,Opie-mVenus tagged, and can be integrated with pBac.

    Depositor

    Omar Akbari

    Article

    Kandul et al GEN Biotechnology (2022) 1:4, 372-385

    Insert

    U6.3-gRNAs[sxl, bTub]
    

    Use

    CRISPR

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGFP-Ub-VPS13D

    Plasmid

    #176462

    Purpose

    expressing GFP-Ub marked VPS13D in mammalian cells. GFP-Ub will be cleaved by DUBs to express untagged VPS13D

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGFP-Ub-VPS13D N3521S

    Plasmid

    #176463

    Purpose

    expressing VPS13D patient mutant N3521S in mammalian cells

    Depositor

    Richard Youle

    Article

    Baldwin et al J Cell Biol. 2021 May 3;220(5). pii: 212

    Insert

    VPS13D (VPS13D Human)
    

    Use

    Tags

    GFP-Ub

    Expression

    Mammalian

    Mutation

    N3521S mutation

    Promoter

    CMV

    Available since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)

    Plasmid

    #105194

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(HC)-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)

    Plasmid

    #105195

    Purpose

    Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)

    Depositor

    Timothy Lu

    Article

    Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

    Insert

    G8p-antiPD1(LC)-1Pe
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. 6xHis(TEV)-Drp1(K569A,K571A)-StrepII

    Plasmid

    #174426

    Purpose

    Expresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K569A, K571A

    Promoter

    T7

    Available since

    Feb. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. IBMc397

    Plasmid

    #161955

    Purpose

    Demonstrates chemically induced dimmerization of acVHH. Expression plasmid for split, evolved T7 RNA polymerase C-lobe fused to acVHH

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3K3-TT-PBAD(SapI-)-B30-His-acVHH-15aa-T7RNAP(181+)-T
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. 6xHis(TEV)-Drp1(K557A,K560A)-StrepII

    Plasmid

    #174425

    Purpose

    Expresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    6xHis(TEV) and StrepII

    Expression

    Bacterial

    Mutation

    K557A, K560A

    Promoter

    T7

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX-4T-1-Xlrbpa-dsRBD(+6)

    Plasmid

    #171548

    Purpose

    Encoding part of the Xenopus Xlrbpa-2 protein from M120 to L202

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Xlrbpa (prkra.L Frog)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    a six amino acid_TRLTEG_insertion in the L1 region

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-4T-1-Staufen-dsRBD(+6)

    Plasmid

    #171546

    Purpose

    Encoding part of the Drosophila Staufen protein from M571 to P656

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Staufen (stau Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    a six amino acid_TRLTEG_insertion in the L1 region

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGEX-4T-1-Staufen

    Plasmid

    #171545

    Purpose

    Encoding part of the Drosophila Staufen protein from M571 to P656

    Depositor

    Yan Zeng

    Article

    Zhang et al Acta Biochim Biophys Sin (Shanghai). 202

    Insert

    Staufen (stau Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p1.2-Zeo-hVKORC1

    Plasmid

    #162776

    Purpose

    Human vitamin K oxidoreductase expression

    Depositor

    Ivan Vorobiev

    Article

    Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

    Insert

    VKORC1 vitamin K epoxide reductase complex subunit 1 (VKORC1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CHO EEF1A1 (Translation Elongation Factor 1 Alpha…

    Available since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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23. pLIC-lyn11-myc-LOV2GIVe (L) (FA)

    Plasmid

    #170627

    Purpose

    For the mammalian expression of mLOV2GIVe lit-mimicking mutation I539E and the GEF-deficient mutation F1685A

    Depositor

    Mikel Garcia-Marcos

    Article

    Garcia-Marcos et al Elife. 2020 Sep 16;9. pii: 60155. doi: 1

    Insert

    Lyn11-myc-LOV2GIVe (L) (FA)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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24. pLV.U6gLacZ.EFS-NS.H2B-RFP

    Plasmid

    #170363

    Purpose

    Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

    Depositor

    Johan Jakobsson

    Article

    Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

    Insert

    H2B-RFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS-NS

    Available since

    June 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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25. SaLgCG

    Plasmid

    #164563

    Purpose

    GFP

    Depositor

    Junwei Shi

    Article

    Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available since

    May 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. IBMc180

    Plasmid

    #161942

    Purpose

    Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    March 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pDONR221 Ub-NLRP1 L1215

    Plasmid

    #166828

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1215 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pDONR221 Ub-NLRP1 L1218

    Plasmid

    #166829

    Purpose

    pDONR221 encoding ubiquitin-tagged NLRP1-CT to generate a L1218 N-terminus (no stop)

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Gateway shuttling vector

    Tags

    Ubiquitin

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. dTAG NLRP1 P1214R

    Plasmid

    #166826

    Purpose

    dTAG (degron tag) NLRP1 for ligand-controlled N-terminal degradation

    Depositor

    Daniel Bachovchin

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    NLRP1 (NLRP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    P1214R

    Promoter

    hPGK

    Available since

    March 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. IBMc226

    Plasmid

    #161944

    Purpose

    Contains Type IIS restriction site free elements for expressing protein to be split in IBM. TetR expression plasmid

    Depositor

    Baojun Wang

    Article

    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

    Insert

    pSB3A5*(BsaI-)-PBAD(SapI-)-tet1-TetR-Ter
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PBAD

    Available since

    March 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge