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LC101: pMVP/PB/Neo-DEST

Plasmid

#121875

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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hsp70: mKate2-DN Kif5a

Plasmid

#105972

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Taverna et al Sci Rep. 2016 Feb 16;6:21206. doi: 10.10

Insert

Kif5a (kif5aa Zebrafish)

Tags

mKate2

Expression

Bacterial

Mutation

truncation of aa416-1033

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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BII-ChBtW-ASCL2

Plasmid

#133375

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

ASCL2 (ASCL2 Human)

Tags

Flag

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-ARP8

Plasmid

#15361

Depositor

Joan Conaway, Ronald Conaway

Article

Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

Insert

actin-related protein 8 (ACTR8 Human)

Tags

FLAG

Expression

Mammalian

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only

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pAG668 lifeAct-pFAST

Plasmid

#172866

Purpose

Expresses LifeAct-pFAST in mammalian cells (actin labeling)

Depositor

Arnaud Gautier

Article

Benaissa et al Nat Commun. 2021 Nov 30;12(1):6989. doi:

Insert

LifeAct-pFAST

Tags

Myc tag (C terminal on insert)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pGWKS7

Plasmid

#139414

Purpose

Fluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mRuby3

Expression

Yeast

Promoter

TEF1

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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BII-ChiNW-ASCL2

Plasmid

#133384

Purpose

Piggybac vector for constitutive expression

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

ASCL2 (ASCL2 Human)

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free)

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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NDUFV3_pcDNA6.2/EmGFP-Bsd

Plasmid

#176946

Purpose

Mammalian expression vector encoding NDUFV3 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

NDUFV3 (NDUFV3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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NHR1+2-Flag pBacPAK8-3

Plasmid

#17324

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Insect

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pCAG MCP-VP64

Plasmid

#92357

Purpose

CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Development. 2018 Feb 23;145(4). pii: de

Insert

MCP-VP64

Use

CRISPR

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL

Plasmid

#65712

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL

Use

Luciferase

Available Since

Feb. 4, 2016

Availability

Academic Institutions and Nonprofits only

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RHBDD1 pmRFP-N2 (1390)

Plasmid

#62049

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

RHBDD1 (RHBDD1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
gh90

Plasmid

#106761

Purpose

expression of gRNA targeting FUT6

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

FUT6 (FUT6 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pMG-miR-125b-1

Plasmid

#58992

Purpose

MSCV based expression vector - co-expresses GFP and human miR-125b-1

Depositor

David Baltimore

Article

So et al Blood. 2014 Aug 28;124(9):1502-12. doi:

Insert

miR-125b-1 (MIR125B1 Human)

Use

Retroviral

Expression

Mammalian

Promoter

MSCV

Available Since

Oct. 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-HLA-B8

Plasmid

#135512

Purpose

Mammalian expression of myc-tagged HLA-B*08:01

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

HLA-B*08:01 (HLA-B Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pmApple-GNG2

Plasmid

#133857

Purpose

Mammalian expression of GNG2 N-terminally tagged with mApple

Depositor

Dave Piston

Article

Foust et al Biophys J. 2019 Nov 5;117(9):1764-1777.

Insert

GNG2 (GNG2 Human)

Tags

mApple

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-2XFLAG,2XHA-CyclinF-puro

Plasmid

#32976

Depositor

Michele Pagano

Article

D'Angiolella et al Nature. 2010 Jul 1;466(7302):138-42.

Insert

CYCLIN F (CCNF Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Promoter

5'LTR

Available Since

Feb. 13, 2012

Availability

Academic Institutions and Nonprofits only
pENTR-AtTAS1c-B/c

Plasmid

#51774

Purpose

Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor

Depositor

James Carrington

Article

Carbonell et al Plant Physiol. 2014 Mar 19.

Insert

AtTAS1c-B/c

Use

Gateway-compatible entry clone

Mutation

A. thaliana TAS1c precursor sequence including a …

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pCEP4-myc-H2-Dd

Plasmid

#135518

Purpose

Mammalian expression of myc-tagged H2-Dd

Depositor

Erik Procko

Article

McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

Insert

H2-Dd (H2-D1 Mouse)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Mutation

C121S

Promoter

CMV

Available Since

Feb. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pUG2K

Plasmid

#35493

Depositor

Ramesh Shivdasani

Article

Horst et al Cancer Res. 2012 Feb 8.

Insert

KRAS(G12V) (KRAS Human)

Use

Lentiviral

Expression

Mammalian

Mutation

G12V

Promoter

Ubiquitin C

Available Since

May 8, 2012

Availability

Academic Institutions and Nonprofits only

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