159,530 results

1. T7-TPase

   Plasmid

   #51818

   Purpose

   For in vitro transcription of Tol2 transposase using T7 Promoter

   Depositor

   Elly Tanaka

   Article

   Khattak et al Nat Protoc. 2014 Mar;9(3):529-40. doi: 1

   Insert

   Tol2 Transposase
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   April 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9-KRAB-MeCP2

   Plasmid

   #110821

   Purpose

   Improved dCas9 repressor-dCas9-KRAB-MeCP2

   Depositor

   Alejandro Chavez, George Church

   Article

   Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

   Promoter

   CMV

   Available since

   July 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Diffusive 41kDa

   Plasmid

   #201335

   Purpose

   Nucleocytoplasmic transport probe

   Depositor

   Pere Roca-Cusachs

   Article

   Andreu et al Nat Cell Biol. 2022 Jun;24(6):896-905. d

   Insert

   EGFP-2PrA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJC175e

   Plasmid

   #79219

   Purpose

   Directed Evolution of Protein-Protein Interactions

   Depositor

   David Liu

   Article

   Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

   Insert

   PPSP gIII, luxAB
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   PPSP

   Available since

   April 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. PA-mCherry1-N1

   Plasmid

   #54507

   Purpose

   Localization: N1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   PA-mCherry1

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 12, 2014

   Availability

   Academic Institutions and Nonprofits only

6. pRC0569 (parts entry vector)

   Plasmid

   #189966

   Purpose

   Cloning vector for generating part plasmids, with mNeonGreen dropout following BsmBI digestion

   Depositor

   Howard Chang

   Article

   Chen et al Nat Biotechnol. 2022 Jul 18. pii: 10.103

   Insert

   mNeonGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   BBa-J23100

   Available since

   Sept. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGL3-Basic-IRES

   Plasmid

   #64784

   Purpose

   Luciferase reporter vector

   Depositor

   Joshua Mendell

   Article

   Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

   Type

   Empty backbone

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. CROPseq-multi-Puro (pRW1441)

   Plasmid

   #216217

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi construct with Puromycin resistance

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. AiP13572 - pAAV-AiE0390h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3572)

   Plasmid

   #208143

   Purpose

   AiE0390h_3xC2 is an optimized enhancer sequence designed to drive AAV-mediated transgene expression in brain astrocytes

   Depositor

   Jonathan Ting, The Allen Institute for Brain Science

   Article

   Mich et al bioRxiv 2023.09.20.558718

   Insert

   SYFP2
   

   Use

   AAV

   Tags

   Expression

   Mutation

   NA

   Promoter

   Beta Globin minimal promoter

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV10-mFoxP1

    Plasmid

    #35170

    Depositor

    Benjamin Blencowe

    Article

    Gabut et al Cell. 2011 Sep 30;147(1):132-46. Epub 20

    Insert

    Foxp1 (Foxp1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. Tol2-mpx:Lifeact-Ruby

    Plasmid

    #45246

    Depositor

    Anna Huttenlocher

    Article

    Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

    Insert

    lifeact-ruby
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    mpx

    Available since

    Oct. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP-Sec23A

    Plasmid

    #66609

    Purpose

    GFP fusion of Sec23A

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec23A (SEC23A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMitoLOC

    Plasmid

    #58980

    Purpose

    Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

    Depositor

    Markus Ralser

    Article

    Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

    Inserts

    mCherry
    GFP
    

    Use

    Tags

    preCOX4 and preSU9

    Expression

    Yeast

    Mutation

    Promoter

    ADH1 and MET17

    Available since

    July 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. p23-puro-EGFP-RPA194

    Plasmid

    #179237

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Wu et al Science. 2021 Jul 30;373(6554):547-555.

    Insert

    RPA194 (POLR1A Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1alpha

    Available since

    March 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. HA-Clover-HIF-1alpha Wildtype

    Plasmid

    #163365

    Purpose

    Express HA- and Clover-tagged HIF-1alpha wildtype plasmid

    Depositor

    Violaine Sée

    Insert

    Hypoxia Inducible Factor 1 alpha (HIF1A Human)
    

    Use

    Tags

    HA- and Clover-tagged

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV Promoter

    Available since

    Jan. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAM2991

    Plasmid

    #40248

    Depositor

    Susan Golden

    Article

    Ivleva et al EMBO J. 2005 Mar 23;24(6):1202-10. Epub

    Insert

    One Step Cloneing Vector for Overexpression
    

    Use

    Cyanobacteria cloning vector

    Tags

    Expression

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENN.AAV.CAG.Flex.GFPsm_myc.WPRE.SV40

    Plasmid

    #98927

    Purpose

    Cre dependent AAV expression of GFP spaghetti monster myc construct

    Depositor

    Loren Looger

    Article

    Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

    Has Service

    AAV1

    Insert

    GFP-smFP-myc
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Jan. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pFB2G

    Plasmid

    #195290

    Purpose

    Bicistronic HSV-TK and mTagBFP resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK 2A mTagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. HS-GFP-PACT

    Plasmid

    #105954

    Purpose

    heat shock inducible transgenesis, PACT is a centriol marker

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    PACT (AKAP9 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. PB-CRISPR

    Plasmid

    #160047

    Purpose

    For cloning sgRNA to knockout a specific gene in piggybac plasmid

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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