We narrowed to 5,421 results for: PEP

1. pETDuet-LahT150

   Plasmid

   #166785

   Purpose

   Cleavage of double glycine motif in peptides to remove the leader sequence

   Depositor

   Wilfred van der Donk

   Article

   Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

   Insert

   LahT150
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. A3Bctd-max

   Plasmid

   #198888

   Purpose

   Express A3Bctd-max BE4max-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
   

   Use

   Tags

   6x His and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-MR1

   Plasmid

   #178603

   Purpose

   Mammalian expression of myc-tagged MR1

   Depositor

   Erik Procko

   Article

   McShan et al Nat Chem Biol. 2022 Jun 20. pii: 10.1038

   Insert

   MR1 (MR1 Human)
   

   Use

   Tags

   Extracellular c-myc epitope ta and Signal peptide…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pStr-KDEL-IRES-ST-mCherry

   Plasmid

   #110727

   Purpose

   Expresses an Str-KDEL Hook for the RUSH system and separately Sialyltransferase-mCherry

   Depositor

   David Stephens

   Article

   McCaughey et al J Cell Biol. 2018 Dec 26. pii: jcb.20180

   Inserts

   Str-KDEL
   STase sialyltransferase (ST6GAL1 Human)
   

   Use

   Tags

   Str Streptavidin binding peptide (SBP) and mCherr…

   Expression

   Mammalian

   Mutation

   contains only amino acids 1 to 43 of ST

   Promoter

   CMV and IRES

   Available since

   Jan. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJRoC30_VP11H

   Plasmid

   #188063

   Purpose

   PROSS-designed versatile peroxidase 11H

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al J Am Chem Soc. 2022 Mar 2;144(8):3564-35

   Insert

   PROSS-designed versatile peroxidase from Pleurotus ostreatus
   

   Use

   Tags

   S. cerevisiae native α-factor prepro-leader signa…

   Expression

   Yeast

   Mutation

   38 PROSS mutations, described in publication

   Promoter

   Available since

   Aug. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. JDW 1050 (pME_V5_GATA1_P2A_H2A_mCherry)

   Plasmid

   #232127

   Purpose

   A middle entry gateway clone containing V5 tagged human GATA1 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA1 (GATA1 Human)
   

   Use

   Gateway cloning

   Tags

   V5

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1-mas-KB-1753-Nluc

   Plasmid

   #158603

   Purpose

   Plasmid expressing KB-1753-Nluc in mammalian cells

   Depositor

   Mikel Garcia-Marcos

   Article

   Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

   Insert

   myristic acid attachment peptide (mas; MGSSKSKTSNS) - linker(GIKLGG) - KB1753 - linker (GGTGGS) - Nluc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pSNAP-CLC-EYFP

   Plasmid

   #38011

   Depositor

   Xiaowei Zhuang

   Article

   Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

   Insert

   Clathrin, light polypeptide (LCa) (Clta Mouse)
   

   Use

   Tags

   EYFP and SNAP26m

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFB-LIC-Bse

   Plasmid

   #26108

   Purpose

   SGC Baculovirus transfer vector with His tag in 22-aa N-terminal fusion peptide with TEV protease cleavage site. Includes sites for LIC cloning, and SacB gene for negative selection

   Depositor

   Opher Gileadi

   Article

   Savitsky et al J Struct Biol. 2010 Oct;172(1):3-13. doi

   Type

   Empty backbone

   Use

   Baculovirus expression

   Tags

   His6 - TEV

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   Oct. 4, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-BiP-Myc-KDEL P495L

    Plasmid

    #27166

    Depositor

    Ron Prywes

    Article

    Shen et al Dev Cell. 2002 Jul . 3(1):99-111.

    Insert

    BiP (HSPA5 Human)
    

    Use

    Tags

    KDEL and Myc

    Expression

    Mammalian

    Mutation

    Peptide Binding Mutant: P495L

    Promoter

    Available since

    Jan. 25, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pLAC

    Plasmid

    #45072

    Purpose

    lacritin-intein fusion plasmid coding for intact mature lacritin without signal peptide

    Depositor

    Gordon Laurie

    Article

    Wang et al J Cell Biol. 2006 Aug 28;174(5):689-700.

    Insert

    Lacritin (LACRT Human)
    

    Use

    Protein expression vector

    Tags

    Intein

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pETcLIC-GFP

    Plasmid

    #183514

    Purpose

    The plasmid contains LIC cassette upstream of folding reporter GFP and TEV-cleavable 10x histidine purification tag. Compatible with T7 promoter-based expression system in E. coli

    Depositor

    Martin Högbom

    Article

    Grave et al Protein Expr Purif. 2022 Oct;198:106132.

    Type

    Empty backbone

    Use

    Tags

    10x His-tag and folding reporter GFP

    Expression

    Bacterial

    Mutation

    Promoter

    T7 lac

    Available since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. mRFP-LAP2β

    Plasmid

    #228029

    Purpose

    lentiviral construct for mRFP-LAP2β expression

    Depositor

    David Pellman

    Article

    Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

    Insert

    LAP2b (Tmpo Rat)
    

    Use

    Lentiviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a(+) 6xHis-3xFlag-IFIT5

    Plasmid

    #53561

    Purpose

    bacterial expression of IFIT5

    Depositor

    Kathleen Collins

    Article

    Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

    Insert

    IFIT5 (IFIT5 Human)
    

    Use

    Tags

    3xFLAG, 6xHIS, and TEV site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 29, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pcDNA3-NPY-ClopHensor

    Plasmid

    #25939

    Depositor

    Daniele Arosio

    Article

    Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

    Insert

    NPY-ClopHensor (NPY Aq. Victoria and Discosoma sp)
    

    Use

    Tags

    NPY and Strep-II

    Expression

    Mammalian

    Mutation

    Neuropeptide Y localization signal

    Promoter

    Available since

    Sept. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVk7-33G1-BioLHis

    Plasmid

    #58949

    Purpose

    backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

    Depositor

    Gavin Wright

    Article

    Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

    Type

    Empty backbone

    Use

    Tags

    6His tag and biotinylation peptide

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSUMO-YHRC

    Plasmid

    #54336

    Purpose

    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.

    Depositor

    Claes Andréasson

    Article

    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d

    Type

    Empty backbone

    Use

    Tags

    6His-SUMO (yeast gene: SMT3)

    Expression

    Bacterial

    Mutation

    Promoter

    T7-LacO

    Available since

    Aug. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-PKI WT

    Plasmid

    #118300

    Purpose

    Expression of PKIa

    Depositor

    Philip Stork

    Article

    Billiard et al J Biol Chem. 2001 Aug 17;276(33):31238-4

    Insert

    PKI wt
    

    Use

    Tags

    Flag and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGG-cTP-TurboID-GFP

    Plasmid

    #209403

    Purpose

    Transiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in planta

    Depositor

    Rosa Lozano-Durán

    Article

    Tan et al J Integr Plant Biol. 2024 Jan 31. doi: 1

    Insert

    PM-3XHA-TurbOID
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    Cauliflower mosaic virus (CaMV) 35S promoter

    Available since

    Jan. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. ScFv-2ERT2-VPH

    Plasmid

    #120556

    Purpose

    Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to 2 tandem ERT2, VP64, P65 and HSF1

    Depositor

    Yu Wang

    Article

    Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

    Insert

    scFvGCN4, GB1, ERT2, VP64, P65 and HSF1
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hPGK

    Available since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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