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GFP-BRPF1

Plasmid

#65382

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRPF1 (BRPF1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
His6-pAG-RTase

Plasmid

#214932

Purpose

Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

Depositor

Chuan He

Article

Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

Insert

fusion protein of protein A/G and MMLV reverse transcriptase (25-497)

Tags

His6

Expression

Bacterial

Mutation

MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

Promoter

T7 promoter

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pLCKO2

Plasmid

#125518

Purpose

Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BsmBI cloning sites and puromycin selection.

Depositor

Jason Moffat

Article

Mair et al Cell Rep. 2019 Apr 9;27(2):599-615.e12.

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pEN_TRE_GFP

Plasmid

#25767

Purpose

Entry vector with TRE promoter driving GFP expression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Gateway Cloning

Tags

GFP

Available Since

July 14, 2010

Availability

Industry, Academic Institutions, and Nonprofits

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pHsp83-NLS-HA-2xMCP-2xTagRFP-T

Plasmid

#71242

Purpose

Used to label reporter mRNAs. Drosophila expression of the MCP protein fused to TagRFP-T.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

NLS-2xMCP-2xTagRFP-T

Use

P element-mediated germline transformation

Tags

TagRFP-T

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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PERK 3: PERK.dC.9E10.pCDNA

Plasmid

#21816

Depositor

David Ron

Article

Harding et al Nature. 1999 Jan 21. 397(6716):271-4.

Insert

PERK (C-term deleted) (Eif2ak3 Mouse)

Tags

Myc (9E10)

Expression

Mammalian

Mutation

amino-acids 582-1081 deleted

Available Since

July 27, 2010

Availability

Academic Institutions and Nonprofits only

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pcDNA-flag-hKDM4A-polyA

Plasmid

#101051

Purpose

in vitro transcription (T7 promoter)

Depositor

Yi Zhang

Article

Chung et al Cell Stem Cell. 2015 Dec 3;17(6):758-66.

Insert

KDM4A (KDM4A Human)

Tags

Flag

Expression

Mammalian

Promoter

T7

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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GFP-BRD1

Plasmid

#65375

Purpose

GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.

Depositor

Kyle Miller

Article

Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do

Insert

BRD1 (BRD1 Human)

Tags

EmGFP and V5

Expression

Mammalian

Mutation

none

Promoter

CMV

Available Since

May 13, 2015

Availability

Academic Institutions and Nonprofits only

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pECIA14

Plasmid

#47051

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Alkaline Phosphatase (human, placental), Flag, HR…

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mNeonGreen

Plasmid

#99131

Purpose

An AAV genome with tet-inducible expression of the fluorescent protein mNeonGreen

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mNeonGreen

Use

AAV

Expression

Mammalian

Promoter

TRE

Available Since

March 15, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-mTurquoise2

Plasmid

#99122

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mTurquoise2 from the CAG promoter

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mTurquoise2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-SARS2-B.1.617.1

Plasmid

#172319

Purpose

expressing SARS-CoV-2 B.1.617.1 (kappa strain) spike protein for pseudovirus production

Depositor

David Nemazee

Article

Cho et al Sci Transl Med. 2021 Oct 20;13(616):eabj

Insert

Spike of B.1.617.1 strain (S SARS-CoV-2)

Expression

Mammalian

Mutation

G142D, E154K, L452R, E484Q, D614G, P681R, Q1071H,…

Promoter

CMV

Available Since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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TPST2-EGFP

Plasmid

#66618

Purpose

Encodes GFP fusion of TPST2 trans Golgi marker (Tyrosyl protein sulfotransferase 2)

Depositor

David Stephens

Article

Spooner et al Biochem J. 2008 Sep 15;414(3):471-84. do

Insert

TPST2 (TPST2 Human)

Expression

Mammalian

Mutation

Insert contains T370A relative to NM_001008566.1

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
1105-Desmoglein2-FLAG-eGFP

Plasmid

#36989

Depositor

Kathleen Green

Article

Klessner et al Mol Biol Cell. 2009 Jan;20(1):328-37. Ep

Insert

Dsg2 (DSG2 Human)

Tags

FLAG and eGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 5, 2012

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-N

Plasmid

#118371

Purpose

Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB515

Plasmid

#74857

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 3xHA (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pHel3

Plasmid

#102961

Purpose

E.coli/H.Pylori shuttle vector

Depositor

Rainer Haas

Article

Heuermann et al Mol Gen Genet. 1998 Mar;257(5):519-28.

Type

Empty backbone

Use

Shuttle vector

Available Since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCFJ1662

Plasmid

#51482

Purpose

miniMos transposable element with HygroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pJA281

Plasmid

#22513

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

Pmex-5::mCherry (w introns w/o stop)

Expression

Worm

Available Since

March 16, 2010

Availability

Academic Institutions and Nonprofits only

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